Esr1 (NM_001302533) Mouse Untagged Clone

CAT#: MC228048

Esr1 (untagged) - Mouse estrogen receptor 1 (alpha) (Esr1), transcript variant 4



  "NM_001302533" in other vectors (1)

CNY 4,750.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms E; ER; ER-; ER-alpha; ERa; ERalpha; ER[; Es; ESR; Estr; Estra; Nr; Nr3a1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228048 representing NM_001302533
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCATGACCCTTCACACCAAAGCCTCGGGAATGGCCTTGCTGCACCAGATCCAAGGGAACGAGCTGG
AGCCCCTCAACCGCCCGCAGCTCAAGATGCCCATGGAGAGGGCCCTGGGCGAGGTATACGTGGACAACAG
CAAGCCCACTGTGTTCAACTACCCCGAGGGCGCCGCCTACGAGTTCAACGCCGCCGCCGCCGCCGCCGCC
GCCGCCTCGGCGCCGGTCTACGGCCAGTCGGGCATCGCCTACGGCCCCGGGTCGGAGGCGGCCGCCTTCA
GTGCCAACAGCCTGGGGGCTTTCCCCCAGCTCAACAGCGTGTCGCCTAGCCCGCTGATGCTGCTGCACCC
GCCGCCGCAGCTGTCTCCTTTCCTGCACCCGCACGGCCAGCAGGTGCCCTACTACCTGGAGAACGAGCCC
AGCGCCTACGCCGTGCGCGACACCGGCCCTCCCGCCTTCTACAGGTCTAATTCTGACAATCGACGCCAGA
ATGGCCGAGAGAGACTGTCCAGCAGTAACGAGAAAGGAAACATGATCATGGAGTCTGCCAAGGAGACTCG
CTACTGTGCCGTGTGCAATGACTATGCCTCTGGCTACCATTATGGGGTCTGGTCCTGCGAAGGCTGCAAG
GCTTTCTTTAAGAGAAGCATTCAAGGACACAATGACTACATGTGTCCAGCTACAAACCAATGCACCATTG
ACAAGAACCGGAGGAAGAGTTGCCAGGCCTGTCGGCTGCGCAAGTGTTACGAAGTGGGCATGATGAAAGG
CGGCATACGGAAAGACCGCCGAGGAGGGAGAATGTTGAAGCACAAGCGTCAGAGAGATGACTTGGAAGGC
CGAAATGAAATGGGTGCTTCAGGAGACATGAGGGCTGCCAACCTTTGGCCAAGCCCTCTTGTGATTAAGC
ACACTAAGAAGAATAGCCCTGCCTTGTCCTTGACAGCTGACCAGATGGTCAGTGCCTTGTTGGATGCTGA
ACCGCCCATGATCTATTCTGAATATGATCCTTCTAGACCCTTCAGTGAAGCCTCAATGATGGGCTTATTG
ACCAACCTAGCAGATAGGGAGCTGGTTCATATGATCAACTGGGCAAAGAGAGTGCCAGGCTTTGGGGACT
TGAATCTCCATGATCAGGTCCACCTTCTCGAGTGTGCCTGGCTGGAGATTCTGATGATTGGTCTCGTCTG
GCGCTCCATGGAACACCCGGGGAAGCTCCTGTTTGCTCCTAACTTGCTCCTGGACAGAGAACATTCTGTC
AAGACTTTGAAAAGAAGCTCTGACAAGGACCTACAAGGACTTCAGACAACTGGGATTCCTTTATGTCAGA
AAGAAGATGGACTACTTTTAAAAAATGCAACCAGGGTACAAGAAAAACCAACCTGTAGTCTGCACTCTCT
AACCAAGGCCAAGAGAGCCTGCCATGTGATACTAGTCCCATCCCCAGAGTCCAGAAATGTAGGTGTCAAA
GATTCCACTCTGCTCGTAGTGAACTCCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001302533
Insert Size 1500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001302533.1, NP_001289462.1
RefSeq Size 2730 bp
RefSeq ORF 1500 bp
Locus ID 13982
Gene Summary This gene encodes an estrogen receptor, a member of the nuclear hormone family of intracellular receptors. The encoded protein, activated by the sex hormone estrogen, is a transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Similar genes in human have been implicated in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
Transcript Variant: This variant (4) differs in the 5' UTR, contains an alternate exon in the 3' coding region and uses an upstream alternate 3' terminal exon, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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