Pi4kb (NM_001293716) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	ESTM41; PI4K-beta; PI4Kbeta; Pik4cb
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227938 representing NM_001293716 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAATCGGCAAACGGCTGGCCACGCTCCCCACTAAAGAGCAAAAAACACAAAGGCTGATCTCAGAGC TTTCCCTGCTCAACCATAAGCTCCCTGCCCGAGTCTGGCTGCCCACGGCTGGCTTTGACCACCACGTGGT CCGTGTGCCCCACACACAAGCTGTTGTTCTCAATTCCAAGGATAAGGCTCCCTACCTGATCTACGTGGAA GTTCTCGAATGTGAAAACTTTGACACAACTAGTGTTCCTGCCCGGATTCCTGAAAACCGAATTCGGAGTA CACGGTCTGTAGAGAACCTGCCTGAATGTGGTATCACTCATGAGCAGCGAGCTGGCAGCTTCAGCACTGT GCCCAATTATGACAATGATGATGAAGCCTGGTCAGTGGATGATATAGGCGAGCTGCAAGTGGAGCTCCCT GAAGTGCACACCAACAGCTGTGACAACATCTCCCAGTTCTCGGTGGACAGCATCACCAGCCAGGAGAGCA AGGAGCCTGTGTTCATTGCAGCAGGGGACATCCGACGGCGCCTTTCAGAACAGCTGGCTCATACTCCTAC AGCCTTCAAACGAGACCCTGAAGACCCTTCTGCAGTTGCCCTCAAAGAGCCCTGGCAGGAGAAAGTGCGG AGGATCAGAGAAGGTTCCCCGTATGGCCATCTTCCCAATTGGCGACTCCTTTCAGTCATTGTCAAGTGTG GAGATGACCTTCGCCAGGAGCTGCTGGCTTTCCAGGTGTTGAAACAACTGCAGTCCATTTGGGAACAGGA GCGAGTGCCTCTTTGGATCAAGCCATATAAGATTCTTGTGATTTCAGCTGACAGTGGCATGATTGAACCA GTAGTCAACGCTGTGTCCATCCACCAGGTGAAGAAACAGTCACAGCTCTCCTTGCTCGATTACTTCCTAC AGGAACATGGCAGTTATACCACTGAGGCATTCCTCAGTGCCCAGCGCAATTTTGTGCAAAGCTGTGCTGG CTACTGCTTGGTCTGCTACCTATTGCAAGTCAAGGACAGGCACAACGGGAACATCCTTCTGGACGCAGAA GGTCACATCATCCACATCGACTTTGGCTTCATCCTTTCCAGCTCACCCCGAAACCTGGGCTTCGAGACAT CAGCCTTTAAGCTGACCACAGAATTTGTGGATGTAATGGGTGGCCTGAACGGTGATATGTTCAACTACTA CAAGATGCTCATGCTGCAAGGGCTGATTGCTGCTCGGAAGCACATGGACAAGGTGGTACAGATTGTGGAG ATCATGCAGCAAGGTTCTCAGCTTCCTTGCTTCCATGGCTCCAGCACCATTCGCAACCTCAAAGAGAGGT TCCACATGAGCATGACTGAGGAGCAGCTGCAGCTGCTGGTGGAGCAGATGGTGGACGGCAGCATGAGGTC CATCACCACCAAACTCTACGATGGCTTCCAGTACCTCACCAATGGCATCATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001293716
Insert Size	1455 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001293716.1, NP_001280645.1
RefSeq Size	2849 bp
RefSeq ORF	1455 bp
Locus ID	107650
UniProt ID	Q8BKC8
Gene Summary	Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation (By similarity). Involved in Golgi-to-plasma membrane trafficking (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation from a downstream start codon compared to variant 1. The encoded isoform (3) has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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