Trim11 (NM_001290988) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227934 representing NM_001290988 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCCCAGACTTGTCCACCAACCTCCAGGAGGAGGCCACCTGCGCCATCTGCCTGGATTACTTCA CCGACCCGGTGATGACCGACTGCGGCCACAACTTCTGCCGCGAGTGCATCCGACGTTGCTGGGGCCAGCC CGAGGGCCCATACGCGTGCCCCGAGTGTCGCGAGCTGTCGGCGCAGAGGAACCTACGGCCCAACCGCCCG CTCGCCAAAATGGCAGAAATGGCACGACGCCTGCACCCGCCTTCTCCGGTCCCGCAGGGCGTCTGTGCGG CGCACCGCGAGCCGCTGACCACCTTCTGCGGCGACGACCTCAGCCTGCTGTGCCCCATCTGCGAGCGTTC GGAGCACTGGACTCACCGGGTGAGACCGCTGCAGGAGGCGGCGGACGACCTCAAGGGGAGGCTGGAGAAG TCACTGGAGCACCTACGGAAACAAATGGAGGACGCAATGCTGTTCCAAGCCCAGGCTGAGGAAACCTGTG CCTTGTGGCAGAAGATGGTGGAGAGCCAGCGGCAGAATGTGCTGGGCGAGTTTGAGAGGCTGCGCCGCCT GTTGGCAGAGGAGGAGCAACAGCTGCTGCAGAAGCTGGAGGAGGAAGAGCTGGAGGTTCTGCCGCGCCTG CGTGAAGGCGCTGCGAGGCTTGGCCAGCAAAGCACGCAGCTGGCAGCCCTCATCTCCGAGCTTGAGAGCC GCTGCCAGCTGCCAGCCCTGGGTTTACTGCAGCTCTGCATTGAGTGCTGCGCCCTGGAGAGAGAAGCGTC TATAGCCAAGGACATTAAGGACGCCCTGTGCAGGGTGCAGGATGTGAAGCTGCAGCCTCCAGCAGTGGTG CCCATGGAGCTGAGGACCGTGTGCCGGGTCCCAGGGCTGGTGGAGACCCTGCGGAGGTTCCGAGGGGACA TAACCCTGGACCCAGACACCGCCAACCCTGAGCTGGTCTTATCTGAGGACCGGAGGAGTGTGCAGCGTGG TGAACAGCGGCAGGCCCTGCCTGACAACCCAGAGCGGTTCGACCCCGGCCCTTGCGTGCTGGGCCAGGAG CGCATTACCTCTGGCCGCCACTACTGGGAGGTGGAAGTCGGGGACCAGACCAGCTGGGCACTCGGCGTGT GTAAAGAAACTGCCAACAGGAAGGAGAAGGGGGAGCTGTCGGCTGGCAATGGGTTCTGGATCCTGGTGTT CCTGGGGAGTTTCTATAATTCCAATGAGCCGGCCTTCTCCCCACTGCGGGACCCTCCCAAGCGTGTGGGG ATTTTTCTGGACTATGAAGCTGGCCATCTCTCATTCTACAGTGCCACGGATGGGTCGCTGCTGTTTATCT TCCCCGAGACCCTGTTCTCAGGGACACTCCGGCCCCTCTTCTCACCTCTGTCAAGCAGCCCGACCCCTAT GACTATCTGCAGGCTGATAGGTGTATCTGGGGACACCCTTGGCCCGCAGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001290988
Insert Size	1452 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290988.1, NP_001277917.1
RefSeq Size	2327 bp
RefSeq ORF	1452 bp
Locus ID	94091
UniProt ID	Q99PQ2
Gene Summary	E3 ubiquitin-protein ligase that promotes the degradation of insoluble ubiquitinated proteins, including insoluble PAX6, poly-Gln repeat expanded HTT and poly-Ala repeat expanded ARX. Mediates PAX6 ubiquitination leading to proteasomal degradation, thereby modulating cortical neurogenesis. May also inhibit PAX6 transcriptional activity, possibly in part by preventing the binding of PAX6 to its consensus sequences. May contribute to the regulation of the intracellular level of HN (humanin) or HN-containing proteins through the proteasomal degradation pathway. Mediates MED15 ubiquitination leading to proteasomal degradation. May contribute to the innate restriction of retroviruses. Upon overexpression, reduces HIV-1 and murine leukemia virus infectivity, by suppressing viral gene expression. Antiviral activity depends on a functional E3 ubiquitin-protein ligase domain. May regulate TRIM5 turnover via the proteasome pathway, thus counteracting the TRIM5-mediated cross-species restriction of retroviral infection at early stages of the retroviral life cycle.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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