Ddx6 (NM_181324) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110001P04Rik; E230023J21Rik; HLR2; mRCK/P54; p54; rck
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227932 representing NM_181324 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCACGGCCAGAACAGAGAACCCTGTTATCATGGGTCTGTCCAGTCAGAACGGCCAGCTGAGGGGCC CTGTGAAGGCCAGTGCTGGCCCTGGAGGAGGGGGCACACAGCCACAGCCACAGCTGAACCAGTTGAAGAA CACCAGCACAATCAATAATGGCACTCCACAGCAAGCCCAGAGCATGGCCGCCACTATTAAACCTGGTGAT GACTGGAAAAAGACATTAAAACTCCCTCCAAAGGATCTAAGAATCAAAACTTCGGATGTGACCTCCACAA AAGGAAATGAGTTTGAAGATTACTGTTTGAAACGAGAGTTGCTAATGGGAATTTTTGAAATGGGCTGGGA AAAACCATCTCCTATCCAGGAGGAGAGCATTCCCATTGCTTTATCTGGTAGGGATATCTTAGCTAGAGCA AAAAATGGAACAGGCAAAAGCGGTGCCTACCTCATCCCCTTACTTGAAAGGCTGGACCTGAAGAAGGACA ATATTCAAGCAATGGTGATTGTTCCCACTAGAGAACTTGCTCTACAGGTCAGTCAGATTTGCATCCAGGT CAGCAAACACATGGGAGGGGCCAAAGTGATGGCAACCACCGGAGGAACCAATTTACGAGATGACATAATG AGGCTTGATGATACAGTGCATGTGGTGATCGCTACCCCTGGCAGGATCCTGGATCTCATCAAGAAAGGCG TGGCAAAGGTTGACCATGTCCAGATGATAGTGCTAGATGAGGCAGATAAATTGTTGTCACAGGATTTTGT GCAGATAATGGAGGATATTATTCTCACACTACCTAAAAATAGGCAGATTTTACTATACTCCGCTACTTTC CCTCTTAGTGTACAAAAGTTTATGAATTCCCATCTGCAAAAACCCTACGAGATAAACCTGATGGAGGAAC TAACTCTGAAAGGAGTAACTCAGTACTACGCATATGTAACGGAGCGCCAAAAAGTGCACTGCCTCAATAC ACTTTTCTCCAGGCTTCAGATAAACCAGTCCATCATTTTCTGCAACTCCTCCCAGAGAGTCGAGCTACTC GCCAAGAAGATTTCTCAGCTGGGCTACTCTTGCTTTTATATCCATGCTAAAATGAGGCAGGAACATCGAA ATCGTGTCTTTCATGATTTCCGAAATGGCTTATGCCGCAATCTTGTTTGCACTGATCTGTTTACCCGAGG TATTGATATACAAGCTGTGAATGTGGTAATAAACTTTGACTTCCCAAAGCTGGCAGAGACCTATCTTCAT CGTATTGGAAGATCAGGTCGCTTTGGTCATCTTGGCTTAGCCATCAACTTGATCACTTATGATGATCGCT TCAACCTGAAGAGTATTGAGGAGCAGCTGGGGACAGAGATCAAACCCATCCCAAGCAACATCGACAAGAG CCTGTATGTGGCAGAATACCACAGTGAGCCTGCAGAAGACGAGAAACCGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_181324
Insert Size	1452 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_181324.3, NP_851841.2
RefSeq Size	6033 bp
RefSeq ORF	1452 bp
Locus ID	13209
UniProt ID	P54823
Gene Summary	In the process of mRNA degradation, plays a role in mRNA decapping (By similarity). Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts (PubMed:26098573).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 5. All five variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.

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