Dlg2 (NM_001243046) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A330103J02Rik; B230218P12Rik; B330007M19Rik; Dlgh2; Gm1197; Gm21505; PSD93
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227926 representing NM_001243046 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTCGCAGAGATGGGAGTTAAAAGGCTGGCTACCATGGGAGCCCCGCAAGGTAGTCCTTCACAAAG GCTCCACTGGCCTGGGCTTCAACATTGTGGGCGGGGAAGATGGAGAAGGTATTTTTGTATCCTTCATTCT GGCCGGTGGACCAGCAGACCTGAGTGGGGAGCTCCAGAGAGGAGACCAGATCTTGTCGGTGAATGGAATC GATCTTCGAGGAGCATCCCATGAGCAGGCAGCTGCAGCACTGAAGGGGGCTGGACAGACTGTGACAATCA TAGCACAATATCAACCTGAAGATTACGCTCGATTTGAGGCCAAAATCCATGACCTACGAGAGCAGATGAT GAACCACAGCATGAGCTCCGGGTCCGGGTCCCTTCGAACCAATCAGAAACGCTCCCTGTATGTCAGAGCC ATGTTTGACTATGACAAGAGCAAGGACAGTGGGCTGCCCAGCCAAGGACTTAGTTTTAAATATGGAGACA TCCTTCATGTCATCAATGCCTCTGATGATGAGTGGTGGCAAGCCAGAAGGGTCACACTAGATGGGGACAG CGAGGAGATGGGCGTCATTCCCAGCAAGCGGAGGGTGGAAAGAAAGGAGCGTGCCCGATTGAAGACAGTG AAGTTCAATGCAAAACCTGGTGTGATTGATTCCAAAGGGTCATTCAATGACAAGCGTAAAAAGAGCTTCA TCTTTTCACGAAAATTCCCATTCTACAAGAACAAGGAGCAGAGTGAGCAGGAAACCAGTGATCCTGAACA ACACGTCTCTTCTAATGCCAGCGATAGCGAAAGTAGCTGCCGAGGACAAGAAGATCTCATTCTTTCCTAT GAACCTGTCACGAGGCAGGAAATAAACTACACGCGGCCAGTGATTATCCTGGGCCCCATGAAGGATCGAA TCAATGATGACTTGATATCTGAATTTCCTGATAAATTTGGCTCCTGTGTGCCTCATACTACGAGGCCAAA GCGTGACTACGAAGTCGACGGCAGAGACTATCACTTTGTCATTTCTAGAGAACAAATGGAGAAAGATATC CAAGAGCACAAATTTATAGAAGCTGGCCAGTACAATGACAATTTATACGGAACCAGTGTGCAATCTGTGA GATTTGTAGCAGAAAGGGGCAAGCACTGTATACTTGATGTATCAGGAAATGCTATTAAGCGGTTACAAGT CGCCCAGCTCTATCCCATTGCCATCTTCATAAAGCCCAAGTCTCTGGAACCTCTGATGGAGATGAATAAG CGTCTGACAGAGGAACAAGCCAAGAAAACTTATGACCGGGCGATTAAACTAGAACAAGAATTTGGAGAAT ATTTTACAGCTATTGTCCAAGGAGATACCTTAGAAGATATTTACAACCAATGCAAGCTTGTTATTGAAGA GCAGTCTGGACCTTTCATCTGGATTCCCTCAAAGGAGAAGTTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001243046
Insert Size	1446 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243046.1, NP_001229975.1
RefSeq Size	6250 bp
RefSeq ORF	1446 bp
Locus ID	23859
Gene Summary	Required for perception of chronic pain through NMDA receptor signaling. Regulates surface expression of NMDA receptors in dorsal horn neurons of the spinal cord. Interacts with the cytoplasmic tail of NMDA receptor subunits as well as inward rectifying potassium channels. Involved in regulation of synaptic stability at cholinergic synapses. Part of the postsynaptic protein scaffold of excitatory synapses.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and has multiple differences in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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