Kcns2 (NM_001271704) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	E130006J24Rik; Kv9.2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227897 representing NM_001271704 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCGCCAGAGCCTGTGGGATGTGTCCGATACCGACGTCGAGGATGGAGAGATCCGCATCAATGTGG GTGGCTTCAAGAGACGGCTGCGTTCCCATACGCTGCTGCGCTTCCCTGAGACACGCCTGGGCCGTCTGCT CCTCTGCCACTCGCGAGAGGCCATTCTGGAACTCTGCGATGACTACGATGACGTTCAGCGTGAGTTCTAC TTCGACCGTAACCCCGAGCTCTTCCCCTATGTGTTGCATTTCTACCACACCGGCAAGCTTCACGTCATGG CTGAGCTGTGCGTCTTCTCCTTCAGCCAGGAGATCGAGTACTGGGGTATCAATGAGTTCTTCATCGACTC TTGCTGCAGCTATAGCTATCACGGCCGCAAAGTGGAACCTGAGCAGGAGAAATGGGACGAGCAGAGTGAC CAGGAAAGCACCACTTCCTCCTTCGATGAGATCTTGGCCTTCTATAATGATGCTTCCAAGTTCGATGGGC AACCCCTGGGCAACTTCCGCAGGCAGCTGTGGCTGGCGTTGGACAACCCAGGCTACTCAGTCCTAAGCAG GGTCTTCAGTGTCCTTTCCATCTTGGTGGTGTTGGGCTCCATCATCACCATGTGCCTCAATAGCCTGCCA GACTTCCAAATCCCTGATAGCCAGGGTAACCCCGGTGAAGACCCCAGGTTCGAAATTGTGGAGCACTTTG GCATTGCTTGGTTCACATTTGAGTTGGTGGCCAGGTTTGCTGTGGCCCCTGACTTTCTTAAGTTCTTCAA GAATGCTCTAAACCTTATTGATCTCATGTCCATTGTCCCATTTTACATAACTCTAGTGGTGAACCTGGTG GTGGAGAGTTCTCCTACCTTGGCTAACTTGGGCAGGGTGGCTCAAGTCCTGAGGCTAATGAGGATCTTCC GAATTCTCAAGCTGGCCAGACACTCCACTGGCCTCCGCTCCTTGGGAGCCACCCTGAAGTACAGCTACAA GGAAGTGGGGTTGCTCTTGCTCTACCTCTCAGTGGGGATTTCCATCTTCTCTGTGGTGGCCTACACCATT GAAAAGGAGGAGAACGAAGGCCTGGCCACCATCCCTGCCTGCTGGTGGTGGGCCACTGTCAGTATGACCA CAGTTGGGTACGGAGATGTGGTCCCAGGGACAACAGCTGGGAAGTTGACTGCCTCTGCCTGCATCTTGGC AGGCATCCTGGTGGTGGTCTTGCCCATCACTTTGATCTTCAATAAGTTCTCCCATTTCTATCGGCGCCAA AAGCAACTTGAGAGTGCTATGCGCAGCTGTGACTTTGGAGATGGAATGAAAGAGGTCCCTTCGGTCAATT TAAGGGACTACTATGCTCATAAAGTTAAGTCCCTCATGGCAAGTCTGACAAACATGAGTAGGAGTTCACC TAGTGAACTGAGTTTAGATGATTCTCTACATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271704
Insert Size	1434 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271704.1, NP_001258633.1
RefSeq Size	5105 bp
RefSeq ORF	1434 bp
Locus ID	16539
UniProt ID	O35174
Gene Summary	Potassium channel subunit that does not form functional channels by itself. Can form functional heterotetrameric channels with KCNB1 and KCNB2; modulates the delayed rectifier voltage-gated potassium channel activation and deactivation rates of KCNB1 and KCNB2 (PubMed:9305895).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.

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