Gck (NM_001287386) Mouse Untagged Clone
CAT#: MC227837
Gck (untagged) - Mouse glucokinase (Gck), transcript variant 2
CNY 4,470.00
货期*
3周
规格
Product images
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经常一起买 (3)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP; MODY2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC227837 representing NM_001287386
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTGGATACTACAAGGAGGGGAGCCCAGTCGTTGACTCTGGTAGAGCAGATCCTGGCAGAGTTCC AGCTGCAGGAGGAAGACCTGAAGAAGGTGATGAGCCGGATGCAGAAGGAGATGGACCGTGGCCTGAAGCT GGAGACCCATCAGGAGGCCAGTGTAAAGATGTTGCCCACCTACGTGCGTTCCACCCCAGAAGGCTCAGAA GTTGGAGACTTTCTCTCCTTAGACCTGGGAGGAACCAACTTCAGGGTGATGCTGGTGAAAGTGGGGGAGG GGGAGGCAGGACAGTGGAGCGTGAAGACGAAACACCAGATGTATTCCATCCCCGAGGACGCCATGACGGG CACTGCGGAGATGCTCTTTGACTACATCTCTGAGTGCATCTCTGACTTCCTGGACAAGCATCAGATGAAA CACAAGAAACTACCCCTGGGCTTCACCTTCTCCTTCCCTGTAAGGCACGAAGACATAGACAAGGGCATCC TGCTCAACTGGACCAAGGGCTTCAAGGCCTCCGGAGCAGAAGGGAACAACATCGTGGGACTTCTCCGAGA TGCTATCAAGAGGAGAGGGGACTTTGAGATGGATGTGGTGGCAATGGTGAATGACACGGTGGCCACAATG ATCTCCTGCTACTATGAAGACCGCCAATGTGAGGTCGGCATGATTGTGGGCACCGGCTGCAACGCCTGCT ACATGGAGGAGATGCAGAATGTGGAGCTGGTGGAAGGCGATGAGGGGCGCATGTGTGTCAACACAGAGTG GGGCGCCTTCGGGAACTCCGGTGAGCTGGACGAGTTCCTCCTGGAGTACGACCGGATGGTGGATGAGAGC TCAGTGAACCCCGGTCAGCAGCTGTACGAAAAGATCATTGGCGGAAAGTACATGGGCGAGCTGGTACGAC TTGTGCTGCTCAAGCTGGTAGAGGAGAATCTTCTGTTCCACGGAGAGGCCTCAGAGCAGCTGCGCACACG TGGTGCTTTTGAGACCCGTTTTGTGTCGCAGGTGGAGAGCGACTCTGGGGACCGAAGGCAGATCCTTAAC ATCCTGAGCACTCTGGGCCTTCGACCCTCTGTCGCCGACTGCGACATTGTGCGCCGTGCCTGTGAAAGCG TGTCCACTCGCGCCGCCCACATGTGCTCAGCAGGACTAGCGGGGGTCATAAATCGCATGCGCGAAAGCCG CAGTGAGGACGTGATGCGCATCACGGTGGGCGTGGATGGCTCCGTGTACAAGCTGCACCCGAGCTTCAAG GAGCGGTTTCACGCCAGTGTGCGCAGGCTGACACCCAACTGCGAAATCACCTTCATTGAATCAGAGGAGG GCAGCGGCAGGGGAGCCGCACTGGTCTCTGCGGTGGCCTGCAAGAAGGCTTGCATGCTGGGCCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001287386 |
Insert Size | 1398 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001287386.1, NP_001274315.1 |
RefSeq Size | 2372 bp |
RefSeq ORF | 1398 bp |
Locus ID | 103988 |
UniProt ID | P52792 |
Gene Summary | Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains an alternate first exon and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) is expressed in liver and has a distinct N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR230048 | Gck (myc-DDK-tagged) - Mouse glucokinase (Gck), transcript variant 2 |
CNY 4,090.00 |
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