Flot2 (NM_001284227) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI573412; Esa; reggie-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227788 representing NM_001284227 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAACTGCCACACGGTGGGCCCCAACGAGGCGCTGGTGGTCTCAGGAGGCTGTTGTGGTTCTGACT ACAAACAGTATGTGTTTGGAGGCTGGGCTTGGGCCTGGTGGTGTATCTCGGACACTCAGAGCCTGAGACT GTGCTGGCCTGGGTGGGTGGAAATACTGAGAGCACCACACCGTGCGGCAGTATATCAGGCATCAGTGCTT ATCTGGATTTCCCTAGAGATTATGACGTTGCAGCCCCGCTGTGAGGACGTAGAGACGGCCGAGGGGGTAG CTTTAACTGTGACGGGTGTCGCCCAGGTGAAGATCATGACGGAGAAGGAGCTCCTGGCTGTAGCCTGTGA ACAGTTCCTGGGCAAGAACGTACAGGACATTAAGAACGTCGTGCTGCAGACCCTGGAGGGGCATCTACGC TCTATCCTAGGGACTCTGACTGTAGAACAGATTTATCAGGACCGAGACCAGTTTGCCAAGCTGGTGCGGG AAGTGGCGGCCCCCGATGTTGGCCGCATGGGCATCGAGATCCTCAGCTTCACCATCAAGGATGTTTATGA CAAAGTAGACTATCTGAGCTCCCTGGGCAAGACACAGACTGCTGTGGTGCAGAGAGATGCAGACATTGGT GTGGCAGAGGCAGAACGGGACGCAGGCATCCGGGAAGCTGAATGCAAGAAGGAGATGCTAGATGTGAAGT TCATGGCAGACACCAAGATCGCTGACTCTAAGAGAGCCTTTGAGCTTCAAAAGTCAGCCTTCAGCGAGGA AGTCAACATCAAGACAGCTGAGGCCCAGTTGGCCTATGAGCTGCAAGGGGCCCGCGAGCAACAGAAGATC CGGCAGGAAGAGATTGAGATTGAGGTCGTACAGCGCAAGAAGCAGATCGCCGTGGAGGCGCAGGAGATCC TCCGCACCGACAAGGAGCTCATCGCCACCGTGCGCCGCCCTGCAGAGGCTGAGGCCCACCGCATCCAGCA GATCGCTGAGGGCGAAAAGGTGAAACAAGTCCTCTTGGCACAAGCAGAAGCTGAGAAGATTCGCAAAATT GGAGAGGCAGAGGCAGCAGTCATTGAGGCAATGGGCAAGGCCGAGGCTGAGCGGATGAAGCTTAAGGCTG AGGCCTACCAGAAGTATGGGGATGCAGCCAAGATGGCCTTGGTGCTGGAGGCCCTGCCCCAGATTGCTGC CAAGATCTCCGCGCCCCTGACTAAAGTCGATGAGATTGTGGTTCTCAGTGGGGACAACAGCAAGGTGACA TCAGAAGTAAACCGGCTGCTAGCAGAACTGCCAGCCTCTGTGCACGCCCTCACCGGTGTGGACCTTTCAA AGATACCCCTGATCAAGAACGCCACTGGTGCACAGGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001284227
Insert Size	1371 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284227.1, NP_001271156.1
RefSeq Size	2711 bp
RefSeq ORF	1371 bp
Locus ID	14252
UniProt ID	Q60634
Gene Summary	May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) encodes the longest isoform (3).

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