Becn2 (NM_001290692) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Beclin-2; Gm104
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227750 representing NM_001290692 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCCTGCCCTCTTCCTGTGCCAGCGCTGCAAAGAACCCCTGAAACTCTTACAGCAGCAGGGGGGGC CCTTGGAAGTCCAGCACCACGCCAATACCCCCACAGAAATTCCAGTTTCTGCAGAGTCCCAAGTCAGGAC CTCCGGCAGACCCCACTCTGACGGTGGCAGGGTGTCCCAGGGAAGTGCCCTCTGCACTTTTACTCTGCTT ACCTCTGGCGGACCGGACTCTGAAGGTGGCACGACGTCCCAGGGAAATGCCTGCTGTACCTTTACTCTGC TTGGTGAATCTGCCTCCATGAGAACTATGAACACTATCCAAAATACTGTCTTAGAGACCTTCGAAATCCT CTCCGACCAAAAGGTGGTGGACCACCCGCTGTGTGTGGACTGTACCGACCATCTTCTGATGCAACTAGAC GACCAGCTCGCTCTCCTGGCATCTGACAACCAGAAGTACAAAAGTTTCCAGGACAGAGAGTTGCTGGTGA GTGAGGAAGAGAGGGAGGCGCTACACGCGGAGCTTTGTGCCGAACTGTCCAGTCTGGAGCAGGAGGAAGC AAGGCTGACCCAGGAGCTAGAGGATTTAGACGGCCATCATGCAAGGGTAGCCGCTGAACTCAGAGCAGCC CAGGCCGAGAGCAAGGAGCTGTATAAACAGCATGAGCAGCACAGGGTCGAGTACTCTGTTTTCAAGATGG AACAGCTGGAGCTGATGGACCAGCTGAGCAGTGTGGAGAACCAGCTGACGTATGCCCTGAGTCAGCAGTA CCGCTTAAGACAGACCAATATCTTCAACGCCACATTTACCATTTCAGATGAGGGTCCCTTGGGCGTTATC AACAATTTTAGGCTGGGCTGTCTCCCCGGGGTCCGGGTGGGCTGGACTGAGATCAGTTCTGCCTGGGGAC AGACAGTCTTACTGCTCTTCAGCTTGTCCAAGATAGCTGGACTGCAATTCCAGAGATATCAACTGGTTCC CTTTGGGGACCATTCCTATCTCAAGTCCTTGACTGGTGATGGCGTGCTGCCCTTATTCTCTGATGGGAGC CACAGCGTCTTCTTGAATAACAAGTTTGACTGTGGGATGAAGGCCTTCCTGGACTGTCTTCAGCAGTTTG TGGAGGAGATTGAGAGGGATGAGAGATGCCCCTGCCTGCCCTACAGGATCCATGTGAAGGAAGGGCTGAT GGAGGATGTCTGGGACAGTGGGGAGTGCTGCTCCATCAGGACCCATCTGAACACGGAGGAGGAGTGGTCC AGGGCTCTGAAATTCATGCTTTCGGACTTGAAGTTGATCCTTGCTTGGGCTTCCTTAAGGTTTTCCAGAG TCCAGAGGCCTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290692
Insert Size	1344 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290692.1, NP_001277621.1
RefSeq Size	1789 bp
RefSeq ORF	1344 bp
Locus ID	226720
UniProt ID	P0DM65
Gene Summary	Involved in 2 distinct lysosomal degradation pathways: acts as a regulator of autophagy and as a regulator of G-protein coupled receptors turnover. Regulates degradation in lysosomes of a variety of G-protein coupled receptors via its interaction with GPRASP1/GASP1.[UniProtKB/Swiss-Prot Function]

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