Uimc1 (NM_001293660) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9430016E08Rik; D330018D10Rik; D630032M02Rik; RIP110; Rxrip110
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227747 representing NM_001293660 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCACGAAGGAAGAAAAAAATTAAAGAGGCCTCTGAAAGCCAGAACTTGGAGAAGAAGGATCTGGAAA CAAGCAGTTGTGTCAGTATAAAGAAGAAGCGTAGACTTGAGGACTTACTCATAGTGATATCCGATAGCGA TGGAGAGGAAACAAAAGAGGAGAATGGATTGCAGAAAACGAAGACAAAACAGTCGAACAGATCAAAGTGT TTGGCTAAAAGAAAAGTTGCACACATGTCAGAAGAAGAACAATTTGCTTTGGCTCTCAAGATGAGTGAGC AGGAAGCTAGGGAGGTGAATAACCAGGAGGAGAAAGAAGAGGAGCTCTTGCGGAAAGCCATTGCTGAAAG CCTGAATTCTTCCCAAGGTCTGTTTGTTGAAGAAACCTCTGAAGAAGGTCTGAAGAGTTCTGAAGGAGAC AACTCTGTGCCCACCACGCAAAGCATTGCAGCTTTGACCAGTAAGAGAAGTTTAGTTCTTATGCCGGAAA GTTCTGCAGAAGAAACCACTGTTTGCCCTGAGACACAGTTAAGTTTCCTTGAACCCCTTGACCTCAATAG AGAAGACTCTCCAGATAGCAGAGAGCTCCCCATTGAAGTAAGGATGGCAGTGGGCGATAAGCAGGTTGCT AATAGGGAAGATTGTATGAAGGAAAACCCTCCTCCTGCAGTCTCATCTAGTACCCGGGTATCCTGCCCAC TGTGTAACCAAGACTTTCCTCCCACAAAGATTGAACAGCATGCCATGTACTGCAATGGTCTGATGGAGCA GGAAACAGTGTTGACTCGGAGACGAAGAGAGGCCAAGAACAAGAGTGACGGTCGGACAGCTGCACAGCCG GCTCTGGATGCCAACAGGAAGGAGAAGTGTTATCTATGTAAGTCCCTGGTTCCACTTGGGGAGTATCAGT GCCATGTGGAGGCCTGTCTCCAGCTTGCAAAGGTTGACAGAGAAGATGGGATTGAAGGGACAAGGAGACC AAGGGTGTGTGCACCTGTGGAGGGGAAACAACAGCAGCGGCTGAAGAAGTCAAAGGACAAAGGCCATAGT CAAGGCCGACTCCTCAGTCTCTTGGAGCAGTCTGAGCATAGGACCACAGGTGTAGAGAAAAAACCCAAGT ATTCGGAAGTAAGAACCTTCAGGATGCCCTCACCAGAGGTGGAAGAGGCTAGCTGCAGCAGAGAGATGCA GAGTACCCTCTCACAGCTCAACTTAAATGAGTCTCCCATCAAGTCTTTTGTTCCTGTTTCAGAAGCTACA AATTGCTTAGTGGACTTTAAAGAACAGTTTGCTTTCCGGTCACGAACTAAATCAGGCAGAGGAAGGAGGA GAAAATCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001293660
Insert Size	1341 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001293660.1, NP_001280589.1
RefSeq Size	2093 bp
RefSeq ORF	1341 bp
Locus ID	20184
UniProt ID	Q5U5Q9
Gene Summary	Ubiquitin-binding protein. Specifically recognizes and binds 'Lys-63'-linked ubiquitin (PubMed:19536136). Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1 (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks two in-frame exons in the coding region, compared to variant 1. The encoded protein (isoform 2) is shorter, compared to isoform 1.

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