Nr1h2 (NM_001285517) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI194859; LXR; LXRB; LXRbeta; LXRBSV; NER1; OR-1; RIP15; Unr; Unr2; UR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227744 representing NM_001285517 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTCCCCCACAAGTTCTCTGGACACTCCCGTGCCTGGGAATGGTTCTCCTCAGCCCAGTACCTCCG CCACGTCACCCACTATTAAGGAAGAGGGGCAGGAGACTGATCCTCCTCCAGGCTCTGAAGGGTCCAGCTC TGCCTACATCGTGGTCATCTTAGAGCCAGAGGATGAGCCTGAGCGCAAGCGGAAGAAGGGGCCGGCCCCG AAGATGCTGGGCCATGAGCTGTGCCGCGTGTGCGGAGACAAGGCTTCGGGCTTCCACTACAACGTGCTCA GCTGTGAAGGCTGCAAAGGCTTCTTCCGGCGCAGTGTGGTCCACGGTGGGGCCGGGCGCTATGCCTGTCG GGGCAGCGGAACCTGCCAGATGGATGCCTTCATGCGGCGCAAGTGCCAGCTCTGCCGGCTGCGCAAGTGC AAGGAGGCTGGCATGCGGGAGCAGTGCGTGCTCTCTGAGGAGCAGATTCGGAAGAAAAGGATTCAGAAGC AGCAACAGCAGCAGCCACCACCCCCATCTGAGCCAGCAGCCAGCAGCTCAGGCCGGCCAGCGGCCTCCCC TGGCACTTCGGAAGCAAGCAGCCAGGGCTCCGGGGAAGGAGAGGGCATCCAGCTGACCGCGGCTCAGGAG CTGATGATCCAGCAGTTAGTTGCCGCGCAGCTGCAGTGCAACAAACGATCTTTCTCCGACCAGCCCAAAG TCACGCCCTGGCCCCTGGGTGCAGACCCTCAGTCCCGAGATGCCCGTCAGCAACGCTTTGCCCACTTCAC CGAGCTAGCCATCATCTCGGTCCAGGAGATTGTGGACTTTGCCAAGCAGGTGCCAGGGTTCTTGCAGTTG GGCCGGGAGGACCAGATCGCCCTCCTGAAGGCGTCCACCATTGAGATCATGTTGCTAGAAACAGCCAGAC GCTACAACCACGAGACAGAATGCATCACGTTCCTGAAGGACTTCACCTACAGCAAGGACGACTTCCACCG TGCAGGCTTGCAGGTGGAATTCATCAATCCCATCTTCGAGTTCTCGCGGGCCATGCGGCGGCTGGGCCTG GACGATGCAGAGTATGCCTTGCTTATCGCCATCAACATCTTCTCAGCCGATCGGCCTAATGTGCAGGAGC CCAGCCGTGTGGAGGCCCTGCAGCAGCCCTACGTGGAGGCGCTCCTCTCCTACACGAGGATCAAGCGCCC ACAGGACCAGCTCCGCTTCCCACGCATGCTCATGAAGCTGGTGAGCCTGCGCACCCTCAGCTCCGTGCAC TCGGAGCAGGTCTTTGCATTGCGACTCCAGGACAAGAAGCTGCCGCCCTTGCTGTCCGAGATCTGGGATG TGCACGAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285517
Insert Size	1341 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285517.1, NP_001272446.1
RefSeq Size	2022 bp
RefSeq ORF	1341 bp
Locus ID	22260
UniProt ID	Q60644
Gene Summary	Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity (PubMed:18055760, PubMed:19520913, PubMed:20427281). Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4) (PubMed:18055760, PubMed:19520913, PubMed:20427281). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8; DLDLR and LRP8 (PubMed:18055760, PubMed:19520913, PubMed:20427281). Interplays functionally with RORA for the regulation of genes involved in liver metabolism (PubMed:18055760, PubMed:19520913, PubMed:20427281). Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a).

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