Cast (NM_001301158) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227739 representing NM_001301158 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCAGTTTCTGTCTTCTACTTTCTGGGAGGGCTCACCTGCTGCAGTGTGGCAAGAAAAGCTTCGTG AGGGTGAACGAAAGGGAGCTGGAGAAACCATCCCTATCCTCCAGGACCATGTAATATGCTCAGAAGAAAG GGAACATGGGTCCAAACACCATGAGGCAAAAGCAAAAGAAGAAAGGCAGGAGAAGTGTGGTGAAGATGAG GACACAGTCCCAGCTGAGTACAGGTTAAAACCAGCAAAGGATAAAGATGGAAAACCACTATTGCCAGAGC CTGAAGAAACATCTAAGAGCCTGAGTGAGTCGGAGCTGATTGGGGAGCTTTCAGCAGATTTTGACCGATC TACATATCAAGACAAACCATCTACGCCAGCTGAAAAGAAATCCAATGACACATCCCAAACTCCTCCGGGG GAGACTGTGCCTCGGGCCTCCATGTGCAGTATACGGTCAGCGCCACCCAAACTAGCATCCTTGAAGGGCG TGGTACCAGAAGATGCTGTTGAAACCTTGGCTGGAAGCCTGGGGACAAGGGAAGCAGATCCAGAACATGA AAAAACTGTGGAGGATAAAGTCAAGGAGAAAGCTAAAGAAGAAGAGCATGAAAAACTTGGTGAAAAAGAA GAAACAGTGCCTCCTGATTATCGACTAGAAGAAGTCAAGGATAAGGATGGAAAACCACTCCTGCCCAAAG AATCCCAGGAACAACTTGCACCCTTAAGCGATGACTTCCTTCTTGATGCCTTGTCTCAGGACTTCTCCAG TCCGGCAAACATATCGTCTCTTGAATTTGAAGATGCCAAACTTTCTGCTGCCATTTCTGAAGTAGTTTCT CAGACACCTGCTCCAAGCACCCATGCAGCAGCTCCACTGCCTGGCACTGAGAAAGACAAAGAACTTGATG ATGCCTTGGATGAACTTTCTGACAGTCTTGGACAAAGGCCGCCTGATCCAGATGAGAACAAACCACTGGA TGACAAAGTGAAGGAGAAAATCAAACCAGAGCATAGCGAGAAACTGGGAGAAAGAGACGACACCATCCCC CCTGAATACAGGCATCTCTTGGATAATGATGGGAAGGACAAACCAGAGAAGCCACCGACTAAGAAAACAG AGAAACCTGATCAGGACCGGGACCCCATTGATGCCCTCTCAGAAGATTTGGATAGCTGCCCCTCAACTAC AGAGACCTCAAAGAATACAGCAAAGGGGAAGAGCAAGAAGACTTCAAGTTCCAAAGCATCCAAGGACGGA GAGAAAACAAAGGACTCTTCCAAGAAGACAGAGGAAGTGTCCAAGCCAAAGGCTAAAGAAGATGCAAGAC ACAGTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001301158
Insert Size	1338 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301158.1, NP_001288087.1
RefSeq Size	2970 bp
RefSeq ORF	1338 bp
Locus ID	12380
Gene Summary	This gene encodes an inhibitor of the calcium-dependent cysteine protease, calpain. This protein plays roles in multiple processes, including apoptosis, cell cycle regulation, and membrane fusion. Multiple protein isoforms exist which contain unique N-terminal domains, and multiple inhibitory domains that share homology with each other. Some isoforms may be tissue-specific. Two different pseudogenes of this gene are found on chromosome 19. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (6) represents use of an alternate promoter and therefore differs in the 5' UTR and 5' coding region, compared to variant 1. These differences cause translation initiation at an alternate start codon and result in an isoform (6) with a shorter and distinct N-terminus. This variant (6) also uses an alternate in-frame splice site in the coding region, compared to variant 1. The resulting protein (isoform 6) has a distinct N-terminus and is shorter than isoform 1.

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