Etv6 (NM_001303102) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AW123102; AW557856; Te; Tel
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227725 representing NM_001303102 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAAGACTCGATCCACCTGCCAACACACCTGCGTTTGCAGCCGATTTACTGGAGCAGAGATGACG TAGCCCAGTGGCTCAAGTGGGCAGAAAATGAGTTTTCTTTAAGGCCCATTGAGAGCAACAAGTTCGAAAT GAATGGCAAGGCCCTCCTGCTGCTGACCAAAGAGGATTTCCGCTACCGATCTCCTCATTCAGGCGACGTG CTCTATGAACTCCTTCAGCATATCCTGAAGCAGAGGAAATCTCGAATGCTCTTCTCACCATTCTTCCCCC CTGGGGACTCTATCCACACCAAGCCAGAGGTCCTCCTGCATCAGAACCATGACGAAGATAACTGTGTCCA GAGGACACCCAGGACGCCCGCGGAGAGCGTGCACCACAACCCTCCCACCATCGAACTCTTACATCGCCCT AGGTCACCCATCACCACAAACCACAGGCCTTCTCCTGACCCCGAACAGCAGCGGCCCCAGCGGTCCCCCC TAGACAACATGAGCCGCCGCCTCTCGCCAGTGGAGAAAGCCCAGGGGCCCAGGCTACAGCAGGAGAACAA CCACCAGGAAACGTACCCCCTGTCAGTGTCTCCTGTCGAGAATAATCACTGCCTGCCCTCAAGCCCCTGG CAGGAGAGCACTCGAGTGATCCAGCTGATGCCCAGCCCCATCATGCACCCTTTGATCCTGAACCCCCGGC ACTCGCACTCGGTGGACTTCAAACAGTCCCGGCACTCCGAGGATGGGATGAATCGGGAAGGGAAGCCCAT CAACCTGTCTCATCGGGAGGACCTGGCTTACTTGAACCACATCATGGTCTCTATGTCCCCACCGGAAGAG CACGCCATGCCCATTGGGAGAATAGCAGACTGTAGACTGCTTTGGGATTATGTCTATCAGTTGCTGTCTG ACAGCCGGTACGAAAACTTCATCCGATGGGAGGACAAAGAATCCAAAATATTCCGGATAGTGGATCCCAA CGGACTGGCTCGACTCTGGGGAAACCATAAGAACAGAACAAACATGACCTATGAGAAAATGTCCAGAGCC CTGCGCCACTACTACAAACTAAACATTATCAGGAAGGAGCCCGGACAAAGGCTTTTGTTCAGGTTCATGA AAACCCCAGATGAGATCATGAGTGGCCGGACAGACCGTCTAGAACACCTCGAGTCTCAAGTGCTGGATGA ACAAACGTACCAAGAGGATGAACCTACCATAGCCTCACCGGTGGGCTGGCCAAGAGGAAACCTGCCCACG GGGACCGCAGGAGGCGTGATGGAAGCAGGCGAGCTAGGGGTGGCTGTAAAGGAAGAGACCCGGGAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001303102
Insert Size	1329 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001303102.1, NP_001290031.1
RefSeq Size	5707 bp
RefSeq ORF	1329 bp
Locus ID	14011
Gene Summary	This gene encodes a transcriptional repressor belonging to the ETS family of proteins. Knockout of this gene in mice results in embryonic lethality due to defective angiogenesis. In humans, this gene is often involved in chromosome rearrangements associated with specific cancers. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2014] Transcript Variant: This variant (2) contains an alternate exon and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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