Taf1a (NM_001277957) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	mTAFI48; TAFI48
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227717 representing NM_001277957 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAGTGATTTCGGTGAAGAGTTGACAAAGCTGGCTGTGGCAGAGGATAACCCAGAAACGTCGGTGC TCTCTAAAACGGGAATGCATTTCCCATGGCTTCACAAGCACGTTGAAGCTGTAGTGACTGGAGGGAAGAA GAGGAAGGATTTTGCTCAGACGACAAGTGCTTGTTTAAGCTTTATCCAGGAAGCCCTGCTGAAGCACCAG TGGCAGCAAGCTGCAGAGTATATGCACAGTTACCTGCAGACCCTGGAGGACTCAGACACCGACAAGAGAC AGGCTGCCCCTGAGATCATTTGGAAGCTTGGAAGTGAAATTCTATTTTACCACCCCAAAAGCAATGTGGA GACTTTTAATAGCTTCGCTGACCGGATGAAAAATATTGGTGTCTTGAATTATTTAAAGATCTCCTTACAA CACGCCTTGTATCTTTTACATCATGGAATGCTTGATGATGCGAACAGGAACCTGAGTAAGGCAGAGACGT GGAGGTATGGAGAAAAGTCGTCTTCTCAGGAAGTGTTGATCAACCTTGTTCAGGCTTACAAAGGGCTTCT GCAGTACTACACTTGGACCAGAAAGAAGATGGAGTTGTCAAAGCTTGATGAAGATGATTACGCTTACGCC GCCAAAACCCGAACCATGCTCAGCCAGAGCTGCAAGACGTCTACAAATATCTGTGCACTGGTCAAAACCC CCGGGGTCTGGGATCCTTTTGTGAAGAGTTATGTGGAGATGCTGGAGTTCTATGGTGATCAAGATGGAGC CCGAGAGATGCTCACCAATTATGCATATGATGAGAAGTTCCCGTCGAACCCCAACGCCCACGTCTACTTA TACGAGTTTCTCAAGAGAGAGAAGGCACCGAGGGCGAAGCTCATAAGTGTTCTTAAGATTTTACATGAGA TTGTGCCATCCCATACACTAATGTTAGAATTCCATACATTGCTTAGAAAATCAGACACAGAAGAACACCA GAAACTGGGCTTGTCTGTGTTATTTGAAGTCCTGGACTTCGCTGGATGCAATAAGAACATAACGGCCTGG AAATACTTGGCAAAGTATCTGAAGCAGATATTAGTGGGAAGCCATCACGAGTGGGTTGAAGAAGAGTGGA AGTCTAGGAGGAACTGGTGGCCAGCTTTTCACTTCAGCTTCTTTTGGGCGAAGAGTGACTGGAAGGCAGA CACTGACTTGGCTTGTTGTAAATATTTTCGGTATATTTTAAAACAAGATCATGAAACCTTGAAGAAGAAA ATCAAGCGAATGAAGAAGTCTGTGAAAAAATACACTATTGTAAATCCAGGTGTTCACACCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001277957
Insert Size	1323 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001277957.1, NP_001264886.1
RefSeq Size	2391 bp
RefSeq ORF	1323 bp
Locus ID	21339
UniProt ID	P97357
Gene Summary	Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

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