Cant1 (NM_001267592) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5830420C20Rik; Apy; Apy1h; D11Bwg0554e; Entpd8; SCA; SCAN-1; Sh; Shapy
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227715 representing NM_001267592 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCATCCAGCCCTTTGACCAGCGGGAATGGAATGAGCCTATGCACTCCCTCCGGATCAGTGTAGGGG GCCTTCCTGTGCTGGCATCCATGACCAAGGCCACGGACCCTCGCTTCCGCCCCCGCTGGCGGGTGATCCT CACGTCCTTTGTGGGTGCTGCCCTCCTCTGGCTGCTGTACTCCCACCATCAGGGCCCAGTGCCAGGCAGG CCCCCCACCCACAATGCGCACAATTGGAGGCTCAGCCAGCAGCGCATCTCTCATTACAACGACACCTACC CCCTCTCCCCCCCACAGAGGACTCCAGGTGGGATTCGGTACCGAATCGCAGTCATTGCTGACTTGGACAC GGGGTCCAGGGCCCAGGAAGAAAACACTTGGTTTAGCTACCTTAAGAAAGGCTACCTGACCCTGTCGGAC AGCGGGGACAGGGTCAGCGTGGAGTGGGATAAAGACCACGGGGTCCTAGAGTCCCACCTGGCAGAAAAGG GGCGGGGCATGGAGCTCTCAGACCTGATTGTCTTCAATGGGAAACTCTACTCCGTGGATGACCGCACAGG GGTCATCTACCAGATCGAAGGCACCAAAGCAGTGCCCTGGGTGATCCTTTCTGATGGCGATGGAACTGTG GAGAAGGGTCAACTGCTGCTACTCAGCCTTGCTCTGAGCCCACCCAGTACAAGGGTCTGTATGCTGAGCA GCAATGACTCCTGGGAACAGAATCTGATATTAGCTTCATGTGTAGCAGGTTTCAAAGCTGAATGGCTTGC TGTGAAGGACGAACACCTGTATGTGGGCGGCCTGGGCAAGGAGTGGACCACCACGACAGGGGAGGTGATG AACGAGAACCCTGAGTGGGTGAAGGTGGTAGGCCACAGGGGCAGTGTGGACCACGAGAACTGGGTGTCCA GCTACAACGCCCTGAGAGCAGCCGCCGGGATCCGACCACCAGGCTACCTCATTCACGAGTCCGCCTGCTG GAGCGACACACTGCAGCGCTGGTTCTTCCTGCCTCGTCGGGCTAGCCACGAACGCTACAGCGAGAAGGAC GATGAACGCAAGGGCAGCAACCTCCTGCTGAGCGCAGCCCAGGACTTCAGGGACATCTCTGTGAGGCAAG TGGGGACTCTGATCCCCACCCACGGCTTCTCATCCTTCAAATTCATCCCCAACACGGATGACCAGATCAT CGTGGCTCTCAAGTCGGAAGAGGACAATGGCAGAATCGCCACCTATGTCATGGCCTTCACGCTGGACGGC CGCTTCCTGCTTCCAGAGACCAAGATCGGCACTGTGAAGTACGAAGGAATAGAGTTCATCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001267592
Insert Size	1323 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001267592.1, NP_001254521.1
RefSeq Size	3068 bp
RefSeq ORF	1323 bp
Locus ID	76025
UniProt ID	Q8VCF1
Gene Summary	The protein encoded by this gene is a calcium-dependent nucleotidase that preferentially hydrolyzes UDP, GDP, and IDP. The encoded protein has low activity with ADP and ATP and shows no activity with AMP and GMP. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2012] Transcript Variant: This variant (5) differs in the 5' UTR and contains an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is longer compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.