Osbpl1a (NM_001252490) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	G430090F17Rik; Gm753; ORP-1; Osbpl1b
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227699 representing NM_001252490 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGAAGGAAAAGACTGTGGTGGGGGAGATGCGCTCTCCAATGGCATCAAAAAGCACAGAACAAGCC TGCCATCACCTATGTTCTCCAGAAACGATTTCAGCATCTGGAGCATCCTCAGAAAATGCATTGGGATGGA ACTGTCCAAGATCACAATGCCAGTGATATTTAATGAGCCTCTGAGCTTCCTGCAGCGGCTAACTGAATAC ATGGAGCACACGTACCTCATCCACAAAGCCAGTTCACTTTCTGATCCTGTGGAAAGGATGCAGTGTGTGG CTGCATTTGCTGTGTCTGCTGTCGCCTCTCAGTGGGAGCGCACCGGAAAGCCCTTCAACCCGCTTCTGGG AGAGACTTATGAATTAGTTCGAGATGACCTTGGGTTTAGGCTCATCTCAGAACAGGTCAGCCATCATCCC CCAATCAGTGCATTCCATGCAGAAGGGCTCAACAATGACTTCATCTTCCATGGCTCAATTTACCCCAAAC TGAAGTTCTGGGGCAAGAGTGTAGAAGCAGAGCCTAAAGGAACCATCACCTTGGAGCTTTTAGACCACAA CGAAGCATACACATGGACAAACCCCACCTGTTGTGTGCATAACATCATCGTGGGCAAGCTCTGGATTGAA CAGTATGGCAACGTGGAAATCATAAACCACAAGACTGGGGACAAATGTGTGCTGAATTTTAAGCCATGTG GTCTTTTTGGCAAGGAATTACACAAAGTTGAAGGCTACATACAAGATAAAAGCAAAAAGAAGCTCTGTGC TCTCTATGGGAAGTGGACTGAGTGCTTGTACAGCGTAGACCCTGCCACTTTTGATGCTTACAAAAAAAAT GATAAGAAAAACACAGAAGAGAAGAAGAACAGCAAACAGACAAGCTCATCTGAGGAGTCTGATGAAATGC CAGTGCCAGATTCCGAGAGCGTATTCATTATCCCTGGAAGTGTGCTCCTGTGGCGGATAGCCCCGCGGCC TCCCAACTCTGCTCAGATGTATAACTTTACCAGCTTTGCCATGGTTTTGAATGAAGTCGACAAGGAGATG GAGAGTGTGATTCCCAAGACTGACTGCAGGCTACGGCCTGACATCAGAGCCATGGAGAACGGAGAGATAG ATCTAGCTAGTGAAGAAAAGAAACGGCTTGAGGAAAAGCAAAGAGCAGCCCGCAAGAACAGGTCCAAGTC GGAAGAGGACTGGAAGACAAGGTGGTTCCATCAAGGTCCTAACCCCTACAGTGGAGCACAGGACTGGATT TATTCTGGCAGCTACTGGGACAGAAACTACTTCAATTTGCCTGATATTTATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252490
Insert Size	1314 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252490.1, NP_001239419.1
RefSeq Size	2703 bp
RefSeq ORF	1314 bp
Locus ID	64291
UniProt ID	Q91XL9
Gene Summary	Binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate. Stabilizes GTP-bound RAB7A on late endosomes/lysosomes and alters functional properties of late endocytic compartments via its interaction with RAB7A. Binds 25-hydroxycholesterol and cholesterol.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a large portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. Variants 3, 4, 5 and 6 encode the same isoform (3), which has a shorter N-terminus compared to isoform 1.

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