Cpvl (NM_001289714) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4933436L16Rik; HVLP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227680 representing NM_001289714 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTCGTGCCAAGTGGAAGATGGTTGTTTCACTGATCTTGTTTATGGTCAGCCCTGGTGATGGACTGT TTCATGCAGTATACCGAAGTATCCTTGTTTCCCAGTCCTTCAAGGGGGATGCAGGACAGCCTCTTTTTCT TAGCCCATACATCAAAAACGGGAAGATCAAGGAGGGGCAAAGGAAGAGCATGGTCAGTCCGTTCCCTGGA ATGAACGACAAGAGTTATGCCGGCTACATCACGGTGAACCAGACTTACAACAGCAACCTCTTCTTCTGGT TCTTTCCGGCTCGGATGCAGCCTGAGGATGCCCCAGTAGTCCTCTGGCTTCAGGGTGGACCTGGAGGTTC ATCCATGTTTGGACTCTTTGTAGAACACGGGCCTTATATTATCACAAGTAACATGACCGTTGTGGCCAGA GACTTCCCTTGGACCTTTACCCTTTCCATGCTCTACATTGATAATCCGGTGGGTACAGGCTTCAGCTTCA CCGATCATTTCCAGGGATACGCCACCAGCGAGGATGATGTAGCCCAAGACTTGTACAGTGCCCTGATTCA GTTCTTCACGTTGTTTCCCGAGTATGCAAAGAATGATTTTTACGTCACTGGCGAGTCTTATGCAGGGAAA TATGTTCCGGCCCTAGCACACTATATCCACTCCCTCAACCCCGTGCGGAAGTTCAAGATTCGCCTAAAAG GAATTGCCATTGGAGATGCATACACTGATCCTGAATCGATCCTGGATAAGCTGCTAGATGGAGATGTAAC AACTGGTTCATCTTTCTTCCAGAATGTGACAGGATGTACCAATTACTACAACATTTTACAGTGCACGGAA CCCAAGGAGCAAAGTTACTTTGCAAAATTCTTGACGCTCCCCCAAGTGAGACAAGCCATCCACGTGGGAA ACCAGAACTTCAGTGACGGTGCTGAGGTTGAGAAGCACCTGCGAGAGGACACAGTGAAGTCGGTGAAGCC CTGGCTGTCTGAGATCATGAATTACTACAAGGTTCTCATCTACAATGGCCAACTGGACATCATCGTGGCG GCTGCCCTGACAGAGCGCTCCTTGATGGCCATGGACTGGAAGGGATCCCGAGCATACAGAAGGGCACGTC GAAAGGTCTGGAAGATCTTCAAATCGGATAATGAAGTGGCTGGTTATGTGAGGCGTGTGGGCAAATTCCA TCAGGTAATCGTCCGAGGTGGAGGACACATTTTGCCCTATGACCAGCCAATGAGATCTTTTGATATGATC AATCGCTTCATCTATGACAGAGGATGGGAACCTTATAATTCATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289714
Insert Size	1305 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289714.1, NP_001276643.1
RefSeq Size	1490 bp
RefSeq ORF	1305 bp
Locus ID	71287
UniProt ID	Q9D3S9
Gene Summary	This gene encodes a member of the serine carboxypeptidase family of proteases that cleave amino acids from the C-terminus of a protein substrate. The human ortholog of this gene, where it was first characterized, was found to be upregulated during the maturation of monocytes to macrophages. The encoded protein may be involved in antigen processing, digestion of phagocytosed proteins in the lysosome and lamellipodium formation. Disruption of this gene in mice was found to cause embryonic lethality. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015] Transcript Variant: This variant (3) uses an alternate splice site in the 5' UTR and lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

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