Eno3 (NM_001276285) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Eno; Eno-3; MSE
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227678 representing NM_001276285 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCATGCAAAAAATCTTCGCCCGGGAAATCCTGGACTCCAGGGGCAACCCCACGGTGGAGGTGGACC TGCACACAGCCAAGGGTCGATTCCGAGCAGCTGTGCCCAGTGGAGCTTCCACGGGTATCTATGAAGCACT GGAACTCCGAGATGGAGACAAAGCACGATACCTGGGGAAAGGAGTGCTGAAGGCTGTGGAACACATCAAC AAGACTCTAGGTCCTGCTCTGCTGGAAAAGAAACTAAGTGTTGTGGATCAAGAAAAAGTTGACAAGTTCA TGATTGAGCTGGACGGGACCGAGAATAAGTCCAAGTTTGGGGCCAACGCCATCCTGGGTGTGTCCCTGGC TGTCTGCAAGGCTGGAGCAGCTGAGAAAGGGGTCCCTCTCTACCGACACATCGCAGATCTTGCAGGCAAT CCCGACCTCGTACTCCCTGTGCCTGCCTTTAATGTGATCAACGGCGGCTCTCATGCTGGAAACAAGCTGG CCATGCAGGAGTTCATGATTCTGCCAGTGGGAGCCAGCTCTTTCAAGGAAGCCATGCGCATCGGCGCTGA GGTCTACCACCACCTCAAGGGGGTCATCAAGGCCAAGTATGGGAAGGACGCCACCAACGTGGGGGATGAG GGTGGCTTTGCACCCAACATCCTGGAGAACAATGAGGCCCTGGAGCTGCTAAAGACAGCCATCCAGGCAG CCGGTTACCCGGACAAGGTGGTGATCGGCATGGATGTAGCTGCGTCTGAATTCTACCGCAACGGCAAGTA TGATCTGGACTTCAAGTCACCCGATGACCCTGCCAGGCACATCAGTGGGGAGAAGCTTGGGGAGCTGTAC AAGAACTTCATCCAGAACTATCCCGTGGTCTCCATTGAGGACCCCTTTGACCAGGATGACTGGGCCACAT GGACCTCATTCCTCTCTGGGGTGGACATCCAGATTGTGGGAGATGACCTCACGGTAACCAACCCCAAGAG GATTGCTCAGGCTGTGGAGAAGAAGGCCTGCAATTGCCTGCTCCTGAAGGTCAACCAGATCGGCTCCGTG ACGGAGTCCATCCAGGCCTGTAAACTTGCACAATCTAATGGCTGGGGAGTGATGGTGAGCCACCGCTCTG GGGAGACCGAAGACACTTTCATCGCTGACCTTGTGGTGGGACTCTGCACAGGACAGATCAAGACTGGTGC TCCCTGCCGTTCAGAGCGTCTGGCAAAATACAACCAGCTTATGAGGATTGAGGAGGCTCTTGGGGACAAA GCTGTCTTTGCTGGAAGAAAGTTCCGTAATCCAAAGGCCAAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276285
Insert Size	1305 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276285.1, NP_001263214.1
RefSeq Size	1506 bp
RefSeq ORF	1305 bp
Locus ID	13808
UniProt ID	P21550
Gene Summary	This gene encodes one of the three enolase isoenzymes found in vertebrates. Enolase is a dimeric enzyme that converts 2-phosphoglycerate to phosphoenolpyruvate as part of the glycolytic pathway. This isozyme is found in skeletal muscle where it is involved in muscle development and regeneration. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 encode the same protein.

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