Trnt1 (NM_001242358) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2410043H24Rik; 2610044E04Rik; 9830143O18Rik; AA408384; C76540; CGI-47; mt-Trat
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227677 representing NM_001242358 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAGCGTCCTGTATCCTTGGCACAGGCAGGTCCTGAGGTGTAGTTGGAGTAGATTGTGCCTTCTGA AGCGATACCTGTTTACAATGAAGTTGCAGTCCCCAGAATTCCAGTCCCTTTTCACCGAAGGATTGAAGAG CCTGACAGAATTATTTGCCAAAGAGAATCATGAATTAAGAATAGCAGGAGGAGCAGTGAGGGATTTACTG AATGGGGTGAAGCCTCAAGATGTGGATTTCGCCACCACTGCCACCCCTACTCAGATGAAGGAGATGTTCC AGTCAGCTGGCATTCGCATGATCAACAACAAAGGAGAGAAACATGGGACTATCACTGCCAGGCTTCATGA AGAAAATTTTGAAGTTACTACACTCCGAATTGATGTTACCACTGATGGAAGACATGCAGAGGTAGAATTC ACAACTGACTGGCAGAAAGACGCTGAACGAAGAGATCTCACTATAAATTCTATGTTTCTAGGTTTTGATG GTACACTGTTTGATTACTTCAATGGTTATGCAGATTTAAAAAATAAAAAGGTTCGATTTGTTGGACATGC AAAACAGAGAATTCAGGAAGATTATCTTCGAATTTTAAGGTATTTCAGGTTTTATGGCAGAATTGTCGAC AGACCTGGTGACCATGACCATGAGACTCTAGAAGCAATTGCAGAAAATGCCAAAGGTTTGGCTGGAATAT CAGGAGAGAGGATTTGGGTAGAACTGAAGAAAATTCTTACTGGTGACCATGTAAACCATTTGATCCACCT GATCTATGATCTTGGTGTGGCTCCTCACATAGGTTTACCTGCTAATGCAAATTTAGAAGAATTTAACAAA GTCAGTAAAAATGTTGAAGGCTTTTCACCAAAACCAATGACTCTTTTGGCTTCTTTATTCAAAGTACAAG ATGATGTCACAAAATTGGACTTGAGATTGAAAATTTCAAAAGAGGAGAAAAACCTGGGTTTATTTATAGT TAAAAACAGGAAAGACTTGATTAAAGCAACAGATAGTTCAGAACCATTGAAACCATACCAAGACTTTGTG ATAGACTCTAGGGAACCAGATGCAACTGCCCGTGTTTGTGAGCTGCTGAAGTACCAAGGAGAGCATGGTC TTCTCAAAGAGATGCAGCAGTGGTCTGTCCCGCCGTTCCCTGTGAGTGGACATGACATCAGGAAAGTGGG TATTTCTTCAGGGAAGGAAATTGGGGCCCTGCTACAGCAGTTGCGTGAACAGTGGAAGAAAAGTGGTTAC CGGATGGAAAAAGATGAACTACTAAGTTACATAAAGAAGACCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001242358
Insert Size	1305 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001242358.1, NP_001229287.1
RefSeq Size	2327 bp
RefSeq ORF	1305 bp
Locus ID	70047
UniProt ID	Q8K1J6
Gene Summary	Adds and repairs the conserved 3'-CCA sequence necessary for the attachment of amino acids to the 3' terminus of tRNA molecules, using CTP and ATP as substrates.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1 and 2 both encode the same isoform (a).

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