Irf8 (NM_001301811) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI893568; IC; Ics; ICSBP; Icsbp1; IRF; IRF-8; My; Myls
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227626 representing NM_001301811 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTGACCGGAACGGCGGGCGGCGGCTGCGGCAGTGGCTGATCGAACAGATCGACAGCAGCATGTACC CGGGGCTGATCTGGGAAAATGATGAGAAGACCATGTTCCGTATCCCCTGGAAGCATGCCGGCAAGCAGGA TTACAATCAGGAGGTGGATGCTTCCATCTTCAAGGCCTGGGCAGTTTTTAAAGGGAAGTTTAAAGAGGGA GACAAAGCTGAACCAGCCACGTGGAAGACGAGGTTACGCTGTGCTCTGAACAAGAGCCCAGATTTTGAAG AAGTGACTGACCGGTCCCAGCTGGACATTTCTGAGCCATATAAAGTTTACCGAATTGTCCCCGAGGAAGA ACAAAAATGCAAGCTGGGCGTGGCACCTGCAGGCTGCATGAGCGAAGTTCCTGAGATGGAGTGTGGCCGC TCAGAGATTGAGGAGCTGATCAAGGAACCTTCTGTGGATGAGTACATGGGTATGACCAAGAGGAGCCCAT CCCCACCAGAGGCCTGCAGGAGCCAGATCCTCCCTGACTGGTGGGTCCAGCAGCCCAGTGCAGGCCTGCC ACTGGTGACCGGATATGCCGCCTATGACACACACCATTCAGCTTTCTCCCAGATGGTCATCAGCTTCTAC TACGGGGGCAAGCTGGTGGGCCAGGCCACCACCACCTGCCTTGAAGGCTGCCGTCTCTCCCTGAGCCAGC CGGGGCTGCCTAAGTTGTATGGGCCGGATGGCCTGGAACCCGTGTGCTTTCCGACGGCCGACACCATCCC CAGTGAGCGGCAGAGGCAGGTGACCCGGAAGCTGTTTGGGCACCTGGAACGTGGCGTGCTACTGCACAGC AACCGCAAGGGCGTGTTCGTGAAGCGGCTGTGCCAGGGCCGCGTGTTCTGCAGCGGCAACGCGGTGGTGT GCAAGGGCAGGCCCAACAAGCTGGAGCGGGACGAGGTGGTGCAGGTCTTTGACACCAACCAGTTCATCCG AGAGCTGCAGCAATTCTACGCCACCCAGAGCCGCCTACCTGACAGCAGGGTGGTCCTGTGCTTCGGGGAG GAGTTTCCGGACACTGTGCCCTTGCGCTCCAAACTCATTCTGGTGCAGGTAGAGCAGCTGTATGCCAGGC AGCTGGTGGAGGAAGCGGGCAAGAGCTGCGGTGCTGGCTCCCTGATGCCAGCCCTGGAGGAGCCCCAGCC GGACCAGGCTTTCCGCATGTTTCCGGATATCTGTACCTCACACCAGAGACCCTTTTTTAGAGAAAATCAA CAGATCACCGTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001301811
Insert Size	1275 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301811.1, NP_001288740.1
RefSeq Size	3391 bp
RefSeq ORF	1275 bp
Locus ID	15900
UniProt ID	P23611
Gene Summary	The protein encoded by this gene is a transcription factor that belongs to the interferon regulatory factor family. Proteins belonging to this family have a DNA binding domain at the amino terminus that contains five well-conserved tryptophan-rich repeats. This domain recognizes DNA sequences similar to the interferon-stimulated response element. The protein encoded by this gene promotes or suppresses lineage-specific genes to regulate the differentation of lymphoid and myeloid lineage cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014] Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.

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