Crhr2 (NM_001288619) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CRF-R2; Crfr2; CRFR2alpha; CRFR2beta
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227557 representing NM_001288619 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGCGGCGCTGCTCCTCAGCCTGCTGGAGGCCAACTGCAGCCTGGCGCTGGCCGAAGAGCTGCTCC TGGACGGCTGGGGAGTGCCCCCGGACCCCGAAGGTCCCTACACCTACTGCAACACGACCTTGGACCAGAT CGGGACCTGCTGGCCACAGAGCGCACCCGGAGCCCTAGTAGAGAGACCGTGCCCCGAGTACTTCAATGGC ATCAAGTACAACACGACCCGGAATGCCTACAGAGAGTGCCTGGAGAACGGGACCTGGGCCTCAAGGGTCA ACTACTCACACTGCGAACCCATTTTGGATGACAAGCAGAGAAAGTATGACCTGCATTACCGAATCGCCCT CATTGTCAACTACCTGGGTCACTGTGTTTCCGTGGTGGCCCTGGTGGCCGCTTTCCTGCTTTTCCTAGTG CTGCGGAGTATCCGCTGCCTGAGGAATGTGATCCACTGGAACCTCATCACCACCTTCATTCTGAGAAACA TCGCGTGGTTCCTGCTGCAACTCATCGACCACGAAGTGCACGAGGGCAATGAGGTCTGGTGCCGCTGCAT CACCACCATCTTCAACTATTTTGTGGTCACCAACTTCTTCTGGATGTTTGTGGAGGGCTGCTACCTGCAC ACGGCCATTGTCATGACGTACTCCACAGAGCACCTGCGCAAGTGGCTTTTCCTCTTCATTGGATGGTGCA TTCCCTGCCCTATCATCATCGCCTGGGCAGTTGGCAAACTCTACTATGAGAATGAGCAGTGCTGGTTTGG CAAGGAAGCTGGTGATTTGGTGGACTACATCTACCAGGGCCCCGTCATGCTTGTGCTGTTGATCAATTTT GTATTTCTGTTTAACATCGTCAGGATCCTGATGACGAAGTTACGAGCATCCACCACGTCCGAGACAATCC AATACAGGAAGGCAGTGAAGGCCACGCTGGTCCTCCTCCCCCTGTTGGGCATCACCTACATGCTCTTCTT TGTCAATCCTGGCGAGGACGACCTGTCCCAGATTGTGTTCATCTACTTCAACTCTTTCCTGCAGTCCTTC CAGGGTTTCTTTGTGTCCGTTTTCTACTGCTTCTTCAATGGAGAGGTGCGCGCGGCCCTGAGAAAGCGGT GGCACCGCTGGCAGGACCACCACGCCCTCCGGGTGCCTGTGGCCCGGGCCATGTCCATCCCTACGTCGCC CACCAGGATCAGCTTCCACAGCATCAAGCAGACAGCTGCTGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI
ACCN	NM_001288619
Insert Size	1236 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001288619.1, NP_001275548.1
RefSeq Size	2607 bp
RefSeq ORF	1236 bp
Locus ID	12922
UniProt ID	Q60748
Gene Summary	G-protein coupled receptor for CRH (corticotropin-releasing factor), UCN (urocortin), UCN2 and UCN3. Has high affinity for UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.