Dennd6a (NM_001285466) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Fam116a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227405 representing NM_001285466 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACTAGTAATGAAGGTTCGGATTCCCACGTGTCATGACAAGCCTGGGACCACGCAGATGGTGCAGT TAACTCAGCAGGCAGATACACACACATCTATTATTTTGCCTACTGTTCACGAGGTGGATCTTTTCAGGTG TTTCTGCCCAGTTTTTCTTCACAGTCAGATGCTCTGGGAGTTGGTGCTCTTGGGAGAGCCCCTGGTGGTC ATGGCGCCATCGCCGTCAGAATCTTCAGAAACTGTACTGGCTCTTGTTAACTGTATCTCTCCATTAAAGT ACTTTAGTGATTTTCGGCCTTACTTCACGATTCATGATAGTGAATTCAAAGAATATACTACCCGTACTCA AGCTCCGCCCTCAGTCATCTTAGGAGTAACCAACCCCTTTTTTGCTAAAACACTACAGCACTGGCCACAC ATTATTCGAATAGGAGATCTTAAACCTGCAGGTGAAATTCCTAAGCAAGTTAAAGTGAAAAAGCTGAAGA ACCTAAAAACTCTGGATTCTAAACCTGGAGTTTATACTTCTTACAAGCCATATCTAAACAGAGATGAGGA GATCATAAAACAACTTCAGAAGGGTATACAGCAGAAGCGTCCTTCTGAGGCCCAAAGTGTTATTCTCCGG CGCTATTTTTTGGAACTAACACAAAGTTTCATCATTCCATTAGAAAGATATGTGGCAAGCTTGATGCCTT TGCAGAAAAGTATTTCTCCTTGGAAGAGTCCACCCCAGTTACGGCAGTTCCTTCCAGAAGAATTTATGAA AACACTTGAAAAAACAGGGCCTCAGCTCACCTCTGGAATAAAGGGCGACTGGATTGGACTTTACCGGCAG TTTCTAAAGTCTCCAAATTTTGATGGCTGGTTCAAGACCCGGCGGAAAGAAATGACTCAAAAATTGGAGG CACTTCATCTAGAAGCTCTTTGTGAAGAGGACCTCCTTCTCTGGATCCAGAAACACACAGAAGTAGAAAC AGTGGACCTTGTGTTGAAGCTGAAAAATAAGTTGTTGCAGGCTGGCCGAGAGAGCTTACCTGTGAAGCCT GACACTGTGGAGAAGTTACGGACACATATAGATGCAATTATCCTGGCCTTACCAGACGACCTGCAAGGCA TACTGCTCAAGACCGGCATGACATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285466
Insert Size	1146 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285466.1, NP_001272395.1
RefSeq Size	6512 bp
RefSeq ORF	1146 bp
Locus ID	211922
UniProt ID	Q8BH65
Gene Summary	Guanine nucleotide exchange factor (GEF) for RAB14. Component of an endocytic recycling pathway that is required for the control of ADAM10 transport, shedding of N-cadherin/CDH2 by ADAM9 or ADAM10 and regulation of cell-cell junctions. Required for RAB14 recruitment to recycling endosomes (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks several exons, uses an alternate 5'-terminal exon, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (c) has a shorter N-terminus, compared to isoform a. Both variants 3 and 4 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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