Htr1d (NM_001285482) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5-HT-1D; 5-HT1D; AI853647; Gpcr14; Htr1db
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227354 representing NM_001285482 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCCTCCAAACCAGTCCCTAGAAGGCCTTCCTCAGGAGGCCTCCAACAGATCCCTGAATGTGACAG GGGCTTGGGACCCAGAGGTCCTGCAGGCTCTCAGAATCTCGCTCGTGGTGGTGCTGTCCGTCATCACACT GGCCACTGTCCTCTCCAATGCCTTCGTCCTTACCACCATTCTACTCACCAAGAAGCTCCACACCCCAGCC AATTATCTCATTGGCTCCTTGGCCACCACGGACCTCCTGGTTTCTATCTTGGTCATGCCCATCAGCATAG CCTACACCACCACCCGCACCTGGAACTTTGGCCAGATCCTGTGTGACATCTGGGTGTCTTCTGACATCAC GTGCTGCACGGCCTCCATCTTGCATCTCTGTGTCATTGCTCTGGACAGATACTGGGCCATCACCGATGCC CTGGAGTACAGCAAGCGTCGAACCGCAGGCCACGCAGCAGCCATGATTGCGGCCGTCTGGATCATCTCTA TTTGTATCTCCATCCCTCCACTCTTCTGGCGGCAGGCCACGGCTCACGAGGAGATGTCCGACTGCCTGGT GAACACATCTCAGATTTCTTACACCATCTACTCGACCTGTGGCGCCTTCTATATCCCATCCATCTTGCTC ATTATCCTGTATGGCCGCATATACGTGGCCGCCCGGAGTCGAATCCTGAACCCACCCTCCCTCTACGGGA AGCGCTTCACCACGGCACAGCTTATCACAGGCTCTGCTGGCTCTTCGCTCTGCTCGCTCAACCCCAGCCT CCATGAGAGCCACACACACACAGTTGGCTCCCCTCTCTTTTTCAACCAGGTGAAAATCAAGCTTGCTGAT AGCATCCTAGAACGCAAGAGGATCTCTGCAGCCCGAGAAAGGAAAGCCACTAAGACCCTGGGCATCATTC TGGGGGCCTTTATCATCTGCTGGTTGCCTTTCTTTGTAGTATCATTGGTCCTCCCCATCTGCAGGGACTC TTGTTGGATCCACCCGGCCCTCTTTGACTTCTTCACGTGGCTAGGTTATTTAAACTCTCTCATTAACCCC GTCATCTACACTGTGTTCAACGAAGACTTTCGACAAGCGTTTCAGAAAGTCGTCCATTTCCGGAAGATCT CATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285482
Insert Size	1125 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001285482.1, NP_001272411.1
RefSeq Size	3079 bp
RefSeq ORF	1125 bp
Locus ID	15552
UniProt ID	Q61224
Gene Summary	G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Regulates the release of 5-hydroxytryptamine in the brain, and thereby affects neural activity. May also play a role in regulating the release of other neurotransmitters. May play a role in vasoconstriction.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, 3 and 4 all encode the same protein.

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