Vasp (NM_001282022) Mouse Untagged Clone

CAT#: MC227297

Vasp (untagged) - Mouse vasodilator-stimulated phosphoprotein (Vasp), transcript variant 3



  "NM_001282022" in other vectors (1)

CNY 3,520.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC227297 representing NM_001282022
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGAGACGGTCATCTGTTCCAGCCGGGCTACTGTGATGCTTTATGATGACAGCAACAAGCGATGGC
TCCCTGCTGGCACTGGTCCCCAGGCCTTCAGCCGCGTCCAGATCTACCACAACCCCACTGCTAACTCCTT
CCGAGTTGTTGGCCGCAAGATGCAGCCGGATCAGCAGGTGGTTATCAACTGTGCCATCATTCGGGGTGTC
AAGTACAATCAGGCCACTCCCATCTTCCATCAGTGGCGAGATGCCCGCCAGGTCTGGGGCCTCAACTTCG
GCAGCAAGGAGGACGCCATACAGTTTGCAACAGGAGGTGGGCCTCCCCCAGCCCCAGCACCCCCTGCCTG
GTCTGCCCAGAATGGTCCCTCCCCAGAGGAGCTGGAACAACAGAAAAGGCAGCCGGAGCATATGGAGCGC
CGGGTCTCCAATGCAGGAGGCCCACCTGCTCCCCCAGCTGGGGGCCCTCCTCCACCTCCAGGACCTCCCC
CTCCTCCAGGTCCCCCCCCACCCCCAGGTCTGCCCTCCTCAGGGGTATCTGGGGCAGGTCATGGAGCAGG
GGCAGCCCCACCCCCTGCACCCCCACTCCCTACAGCACAGGGCCCCAATAGTGGGGGTTCCGGGGCCCCA
GGCCTGGCTGCTGCCATTGCTGGAGCCAAACTCAGGAAAGTGAGCAAGCAGGAGGAGGCCTCTGGGGGGC
CCCTGGCCCCCAAAGCTGAGAACAGTCGAAGCACTGGTGGGGGGCTTATGGAAGAGATGAACGCCATGCT
GGCCCGGAGAAGAAAAGCCACACAGGTTGGGGAGAAGCCCCCCAAAGACGAGTCAGCCAGTGAGGAGTCA
GAGGCCCGACTCCCTGCCCAGAGTGAACCTGTGAGAAGACCCTGGGAGAAGAACAGCACAACCTTGCCAA
GGATGAAGTCGTCCTCTTCTGTGACTACCTCCGAGGCCCACCCCTCGACGCCCTGCTCCAGTGATGACTC
CGACTTGGAGAGGGTGAAGCAGGAGCTTCTGGAAGAGGTGCGGAAGGAGCTACAGAAAATGAAAGAGGAA
ATCATCGAAGTCTTTGTCCAGGAGCTGAGGAAGCGGGGTTCTCCTTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001282022
Insert Size 1098 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001282022.1, NP_001268951.1
RefSeq Size 2237 bp
RefSeq ORF 1098 bp
Locus ID 22323
UniProt ID P70460
Gene Summary Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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