Rbfox3 (NM_001285436) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Fox-3; Hrnbp3; NeuN; Neuna60
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227269 representing NM_001285436 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGCCCTACCCCCCTGCCCAGTACCCTCCTCCGCCTCAGAATGGAATCCCAGCTGAATATGCCC CACCTCCGCCTCATCCCACCCAGGACTACTCCGGCCAGACCCCTGTTCCCCCCGAGCACGGCATGACCCT CTACACACCCGCACAGACTCATCCTGAGCAGCCAGGCACTGAGGCCAGCACACAGCCCATTGCTGGGACC CAGACGGTGCCGCAGGCAGATGAAGCAGCACAGACAGACAACCAGCAACTCCACCCTTCCGACCCCACGG AGAAGCAGCAGCCCAAACGACTACATGTCTCCAACATCCCCTTCCGGTTCAGGGACCCCGACCTGCGGCA AATGTTCGGGCAATTCGGGAAAATTTTAGACGTGGAGATCATTTTTAACGAGCGGGGCTCCAAGGGTTTT GGGTTTGTAACTTTTGAAACTAGCTCAGATGCTGACCGAGCCCGGGAGAAGCTGAATGGGACGATCGTAG AGGGACGGAAAATTGAGGTCAATAATGCCACAGCCCGGGTCATGACCAATAAGAAGCCTGGGAACCCATA TGCCAATGGCTGGAAGCTAAACCCTGTGGTAGGAACAGTCTATGGGCCTGAATTCTATGCAGTGACCAGT TTCCCCTACCCCACCACGGGCACCGCCGTTGCCTACCGGGGTGCACACCTGCGGGGCCGGGGCCGTGCTG TGTATAATACATTTCGAGCTGCACCACCTCCACCCCCCATTCCAACTTATGGAGCGGCACTGGAGCAAAC GCTTGTTAAAATGCCAGTCCCGTGGGCGGGGCTGGCACCGTGCCCCCTCCCTCCTCAGCAGACACCGGAG CCGGCCTACCCCACCTCTCCAGCGTTCCCACCACTCTCTTGTCCGTTTGCTTCCAGGGTCGTGTATCAGG ATGGATTTTATGGTGCTGAGATTTATGGAGGCTATGCAGCTTACAGATATGCTCAGCCAGCAGCCGCCAC CGCAGCAGCCTACAGTGACAGTTATGGTAGAGTCTATGCCGCTGCTGATCCTTACCATCACACCATCGGC CCCACAGCAACCTACAGCATCGGAACCATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285436
Insert Size	1083 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001285436.1, NP_001272365.1
RefSeq Size	3108 bp
RefSeq ORF	1083 bp
Locus ID	52897
UniProt ID	Q8BIF2
Gene Summary	Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) uses an alternate in-frame splice junction at the 5' end of an exon and has an alternate 3' UTR and coding sequence compared to variant 1. The resulting isoform (4) contains an alternate internal segment and is shorter at the C-terminus compared to isoform 1.

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