Ppp1r8 (NM_001290725) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6330548N22Rik; AU044684; NIPP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227212 representing NM_001290725 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCAGCCGTGAACTCCGGCTCCAGCCTCCCGCTGTTCGACTGCCCGACTTGGGCAGGTAAACCAC CACCTGGCTTACATCTGGATGTGGTGAAAGGAGACAAGCTAATTGAGAAGCTGATTATTGATGAGAAAAA GTATTACTTATTTGGGAGGAACCCCGATCTGTGTGACTTCACTATCGACCACCAGTCATGCTCTCGAGTC CACGCAGCGCTGGTGTACCACAAACACCTGAAGAGAGTTTTCTTGATAGACCTCAACAGCACACATGGCA CTTTCTTGGGTCACATTCGGCTGGAACCTCACAAGCCTCAACAGATTCCCATCGATTCTACGGTCTCATT TGGCGCGTCCACGAGGGCATACACTCTGCGAGAGAAGCCTCAGACATTGCCATCGGCTGTGAAAGGAGAT GAGAAGATGGGCGGAGAGGATGATGAACTCAAGGGCTTGTTGGGACTTCCCGAAGAGGAGACCGAGCTGG ATAACCTGACAGAGTTCAACACTGCCCACAACAAGCGGATTTCAACCCTCACTATTGAGGAGGGGAACCT GGACATCCAGAGACCAAAGAGGAAGAGGAAGAACTCAAGGGTGACCTTCAGTGAGGATGACGAGATCATC AACCCAGAGGACGTGGATCCGTCTGTTGGTCGGTTCCGGAACATGGTGCAGACAGCAGTGGTTCCAGTCA AGAAGCGGATGGAGGGCTCTGGCTCTCTGGGCCTGGAGGAGTCGGGGAGCAGGCGCATGCAGAACTTTGC CTTCAGTGGAGGACTATATGGGGGCCTGCCCCCCACACACAGTGAAACGGGCTCCCAGCCGCATGGCATT CATGGGACAGCGCTCATTGGTGGCTTGCCCATGCCATACCCGAACCTCGCCCCTGATGTGGACTTGACTC CTGTTGTGCCATCAGCAGTGGCCATAAATCCCACACCAAACCCTGCAGTCTATAACCCTGAGGCTGTGAA TGAACCCAAGAAGAAGAAATACGCGAAGGAGGCTTGGCCGGGCAAGAAGCCCACACCTTCCTTACTGATT TGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290725
Insert Size	1053 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290725.1, NP_001277654.1
RefSeq Size	2057 bp
RefSeq ORF	1053 bp
Locus ID	100336
UniProt ID	Q8R3G1
Gene Summary	Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Essential for cell proliferation and early embryonic development.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. It encodes isoform 2, which is shorter by an amino acid, compared to isoform 1.

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