Map2k7 (NM_001291783) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5930412N11Rik; JNKK 2; Jnkk2; MAPKK 7; Mapkk7; MEK 7; Mek7; Mkk7; Prkmk7; sek2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227180 representing NM_001291783 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGGGCTCCCATCAACCTTGTTCACACCGCGCAGTATGGAGAGCATCGAGATTGACCAGAAGCTGC AGGAGATCATGAAGCAGACAGGGTACCTGACTATCGGGGGCCAGCGTTATCAGGCAGAAATCAATGACTT GGAGAACTTGGGTGAGATGGGCAGTGGTACCTGTGGTCAGGTGTGGAAGATGCGGTTCCGGAAGACAGGC CACATCATTGCTGTTAAGCAAATGCGGCGCTCTGGGAACAAGGAAGAGAATAAGCGCATTTTGATGGACC TGGATGTAGTACTCAAGAGCCATGACTGCCCTTACATCGTTCAGTGCTTTGGCACCTTCATCACCAACAC AGACGTCTTTATTGCCATGGAGCTCATGGGCACATGTGCAGAGAAGCTGAAGAAACGAATGCAGGGCCCC ATTCCAGAGCGAATCCTGGGCAAGATGACTGTGGCGATTGTGAAAGCACTGTACTATCTGAAGGAGAAGC ATGGCGTCATCCATCGCGATGTCAAACCCTCCAACATCCTGCTAGATGAGCGGGGCCAGATCAAGCTCTG TGACTTTGGCATCAGTGGCCGCCTTGTTGACTCCAAAGCCAAAACACGGAGTGCTGGCTGTGCTGCCTAT ATGGCTCCCGAGCGCATCGACCCTCCAGATCCCACCAAGCCTGACTATGACATCCGAGCTGATGTGTGGA GCCTGGGCATCTCACTGGTGGAGCTGGCAACAGGACAGTTCCCCTATAAGAACTGCAAGACGGACTTTGA GGTCCTCACCAAAGTCCTACAGGAAGAGCCCCCACTCCTGCCTGGTCACATGGGCTTCTCAGGGGACTTC CAGTCATTTGTCAAAGACTGCCTTACTAAAGATCACAGGAAGAGACCAAAGTATAATAAGCTACTTGAAC ACAGCTTCATCAAGCACTATGAGATACTCGAGGTGGATGTCGCGTCCTGGTTTAAGGATGTCATGGCGAA GACCGAGTCCCCAAGGACTAGTGGAGTCCTGAGTCAGCACCATCTGCCCTTCTTCAGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291783
Insert Size	1041 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291783.1, NP_001278712.1
RefSeq Size	3394 bp
RefSeq ORF	1041 bp
Locus ID	26400
UniProt ID	Q8CE90
Gene Summary	Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Part of a non-canonical MAPK signaling pathway, composed of the upstream MAP3K12 kinase and downstream MAP kinases MAPK1/ERK2 and MAPK3/ERK1, that enhances the AP-1-mediated transcription of APP in response to APOE (PubMed:28111074).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (6) differs in the 5' UTR and coding sequence and retains the last intron compared to variant 1. The resulting isoform (6) is shorter at the N-terminus and has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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