Mgme1 (NM_001289631) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	8430406I07Rik; AI426476
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227123 representing NM_001289631 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAACTGCCTCTGACCTTCTGCAGGCTGCTTAGCAGGTTAAACAGATTTTCTGTAAAAGCAAGCCCTC CTGTGAGTTTCTCCACTTTCTCTTATTTGTGTAGCCAGAAGAAGAAAAACTCTTACGAAGCAGTAGACCA AGCGAAGTACTCTCGTTTAGTACGCTCTGTCTTGTCCAGAGGCCCGGCCCAGACTCCAGAGTCGTTGTTC AAGGAAGATGATGTACTGTATGGACCAGTGAGTAAGCATAAGGCTGCAGAGCCGGAGCCACAGGCCAGAG TCCCACAACACTGCTTTCCTATCTTCAATGAAGAGAGAACCGGGAAACCACACACAGATGCTTCTTCAAG CCCTTTGAAGATCCCTTTGCAAAGGAACTCGATACCCAGTGTGACCCGCATCCTTCAGCAGACCATGCCA CCTGAACAGAGCTTCTTTTTGGAGAGGTGGAAAGAGCGGATGGTTCTGGAGCTGGGAGAAGACGGGTTTG CAGAATATACTTCAAATGTGTTTTTACAAGGCAAACAGTTCCATAAAGCCTTGGAAAGCATACTGTCACC CCAGGAGAACCTAACAGGGGGAGAAGAGCACCCCCAGTGTGGCTACATCGAAAGCATCCAGCATATTCTG ACAGAAATCAGTGGTGTGCAAGCTCTGGAGAGTGCCGTCCAGCATGAGGCCTTGAAGTATGTAGGGCTGC TGGACTGTGTGGCTGAGTACCGGGGCAAGCTGTGTGTGATTGATTGGAAGACATCAGAAAAACCAAAACC TTTAATTCGAAATACATATGACAACCCGCTGCAAGTTGTGGCGTACATGGGTGCCGTAAACCATGATGCC CACTACAGTTTTCAGGTTCAGTGTGGATTAATTGTGGTGGCCTATAAGGATGGGTCCCCTGCGCACCCTC ACTTCATGGATGAAGAGCTCTGTTCCAAGTATTGGGCCAAGTGGCTTCTCCGACTAGAAGAATATACAGA AAAGCAAAAGAACCTGAGCGCTCCAGAGCCAGCGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289631
Insert Size	1017 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289631.1, NP_001276560.1
RefSeq Size	2778 bp
RefSeq ORF	1017 bp
Locus ID	74528
UniProt ID	Q9CXC3
Gene Summary	Metal-dependent single-stranded DNA (ssDNA) exonuclease involved in mitochondrial genome maintenance. Has preference for 5'-3' exonuclease activity but is also capable of endoduclease activity on linear substrates. Necessary for maintenance of proper 7S DNA levels. Probably involved in mitochondrial DNA (mtDNA) repair, possibly via the processing of displaced DNA containing Okazaki fragments during RNA-primed DNA synthesis on the lagging strand or via processing of DNA flaps during long-patch base excision repair (By similarity). Specifically binds 5-hydroxymethylcytosine (5hmC)-containing DNA in stem cells.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. All four variants encode the same protein.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.