Rprd1b (NM_001291134) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610304G08Rik; 2810446G03Rik; Crept
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227049 representing NM_001291134 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTCCTTCTCAGAATCGGCGCTGGAAAAGAAGCTCTCAGAGCTGAGCAACTCTCAGCAGAGCGTGC AGACCCTCTCCCTGTGGCTCATCCACCACCGCAAGCACGCAGGACCCATCGTCTCCGTGTGGCACCGCGA GCTCCGCAAAGCCAAATCAAATAGAAAGCTTACTTTTCTATACTTAGCAAATGATGTCATCCAAAACAGT AAAAGGAAAGGACCTGAGTTCACAAGAGAATTTGAGTCTGTGCTTGTGGATGCTTTTTCTCATGTTGCCA GAGAGGCAGATGAAGGCTGTAAAAAACCTTTAGAAAGATTGCTGAACATCTGGCAAGAACGAAGTGTGTA CGGCGGCGAGTTCATACAGCAGCTGAAGCTGTCTATGGAGGACTCCAAGAGCCCTCCCCCCAAAGCAGCA GAAGAGAAGAAGTCTCTAAAACGAACTTTTCAGCAGATACAAGAGGAGGAAGATGATGACTACCCTGGAA GCTACTCTCCCCAAGACCCTTCTGCAGGCCCTCTCTTGACTGAGGAGTTAATCAAAGCTTTGCAGGATCT GGAAAATGCTGCGTCAGGGGATGCTACTGTCCGACAGAAGATCGCTTCCCTGCCTCAGGAAGTGCAAGAC GTGTCGCTGCTAGAGAAAATTACAGACAAAGAGGCAGCTGAACGTCTTTCAAAAACAGTAGATGAAGCAT GTCTGTTACTAGCGGAATATAACGGGCGCCTGGCAGCGGAACTGGAAGACCGGCGCCAGCTGGCTCGGAT GCTGGTGGAATACACCCAGAACCAGAAAGAGGTTTTGTCAGAAAAAGAGAAAAAACTAGAAGAGTATAAA CAGAAGCTTGCTCGAGTAACCCAGGTCCGCAAGGAACTCAAGTCCCACATTCAGAGCTTGCCAGACCTTT CGCTGTTGCCTAATGTCACAGGGGGCCTGGCACCTCTGCCCTCTGCTGGTGACCTCTTTTCAACTGACTA G ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291134
Insert Size	981 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291134.1, NP_001278063.1
RefSeq Size	4520 bp
RefSeq ORF	981 bp
Locus ID	70470
UniProt ID	Q9CSU0
Gene Summary	Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. Transcriptional regulator which enhances expression of CCND1. Promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. Prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (a).

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