Upp2 (NM_001289660) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1700124F02Rik; AI266885; UDRPASE2; UP2; UPASE2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227040 representing NM_001289660 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCGCCCGCCGCCCCCGACCCCCGACCCTTCGGGCCGCCCGGGCTGTGGGCTGCTGCGAGCCGGGCT GGCGGGACGCGGGGGAGGCGCGCGGCACGGGGAGCGGCCAGAGTATGGCTTCCATTTTGCCTGCTTCTAA TAGATCCATGAGACCTGACAAGAATACATACGAGAGAAAACGGTCTGTGTATGTTAAGAATCCATACCTG GAAGGCATGGACGAAGACATTCTCTATCACTTGGATTTAGGAACCAAAACACACAACCTACCAGCAATGT TTGGGGATGTAAAGTTTGTCTGCGTTGGTGGGAGCCCGAACAGAATGAAGGCATTTGCACAGTTTATGCA CAAGGAACTCCGGTTGGAGGGAGATGGAGAAGACATTGAAGACATCTGTGCGGGGACAGACAGATACTGC ATGTTCAAAACAGGCCCCGTGCTCTCCGTCAGTCACGGGATGGGCATCCCCTCCATTTCCATTATGCTCC ACGAACTCATCAAATTACTCCACCATGCACACTGCTGTGATGTTACCATCATCAGAATTGGAACGTCAGG GGGAATTGGGATTGCACCAGGCTCCGTTGTAATAACAGATACAGCTGTAGACTCCTTCTTCAAGCCTCGA TTTGAGCAGGTCATCTTGGATAACGTGGTCACCCGAAGCACTGAGCTTGACAAAGAACTGGCTAATGATC TGTTCAACTGTAGCAGAGAAATCCCCAATGTTCCAACCCTCATCGGACACACGATGTGTACCTATGACTT TTATGAAGGCCAAGGTCGCCTAGATGGGGCGTTATGCTCCTTTTCGAGAGAGAAAAAGCTAGACTACTTG AAGAGAGCATACAGAGCTGGTGTCAGGAACATTGAAATGGAATCGACTGTGTTCGCAGCCATGTGTGGCC TGTGTGGCCTGAGAGTAGTTCCTATGTCAGCAAATAGTGTCCGGCTGAAAACCTGCAAACTACTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289660
Insert Size	978 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289660.1, NP_001276589.1
RefSeq Size	2219 bp
RefSeq ORF	978 bp
Locus ID	76654
Gene Summary	Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains alternate 5' and 3' terminal exons, initiates translation at an alternate start codon and lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. It encodes variant 2, which is shorter and has distinct N- and C-termini, compared to isoform 1.

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