Deaf1 (NM_001282076) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AU042387; C230009B13Rik; NUDR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226953 representing NM_001282076 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGAGGCTGTGTGCTTGCCTGGCAGCAGTGCTCTTCATGGATTTCTTCAGAAAATGTCTTCTCTACC ATTCTGAGGGCTCCTGTGGACGTCTGCTCTCTGCAGTAAGTGGCCCTGTCAGGCTCTTCGTCCCTTATAA AAGGCGCAAGAAAGAGAATGAGCTGCCCACAACTCCAGTGAAGAAGGATTCCCCCAAGAACATCACCCTG CTTCCTGCCACGGCGGCCACCACCTTCACTGTGACACCCTCAGGACAGATCACTACCTCTGGAGCACTGA CCTTTGACAGAGCATCCACTGTAGAGGCCACTGCTGTCATCTCTGAGAGCCCAGCCCAAGGTGATGTCTT TGCAGGAGCCACAGTGCAAGAGGCAGGTGTGCAGCCTCCCTGCAGGGTTGGCCACCCTGAACCCCACTAC CCTGGCTATCAGGACAGCTGCCAGATTGCCCCGTTTCCAGAAGCTGCATTGCCAACATCACACCCCAAAA TTGTCCTGACATCGCTGCCCGCATTGGCCGTGCCACCGTCCACCCCCACCAAAGCTGTCTCTCCCACCGT GGTCAGTGGGCTGGAGATGTCAGAACATCGGAGCTGGCTGTACCTGGAAGAGATGGTCAACTCCCTACTC AACACAGCTCAGCAGCTGAAGACGCTGTTTGAACAAGCCAAGCAGGCGAGCTCTTGCAGGGAAGCTGCTG TGACCCAGGCGAGAATGCAGGTTGATACAGAGAGGAAAGAGTCATGTGTCAACTGCGGCCGGGAGGCCAT GAGTGAGTGTACCGGCTGCCACAAGGTTAACTACTGCTCTACGTTCTGCCAGCGCAAGGACTGGAAAGAC CATCAGCATGTGTGTGGCCAGTCAGCGTCTGTCACTGTCCAGGCTGATGACGTCCATGTTGAAGAAAGTG TGATAGAAAAAGTTGCTGTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001282076
Insert Size	933 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282076.1, NP_001269005.1
RefSeq Size	2316 bp
RefSeq ORF	933 bp
Locus ID	54006
Gene Summary	Transcription factor that binds to sequence with multiple copies of 5'-TTC[CG]G-3' present in its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE) 5'-AGGGTTCACCGAAAGTTCA-3'. Activates the proenkephalin gene independently of promoter binding, probably through protein-protein interaction (By similarity). Regulates epithelial cell proliferation and side-branching in the mammary gland. Required for neural tube closure and skeletal patterning. Controls the expression of peripheral tissue antigens in pancreatic lymph nodes. Isoform 1 displays greater transcriptional activity than isoform 2. Isoform 2 may inhibit transcriptional activity of isoform 1 by interacting with it and retaining it in the cytoplasm. Transcriptional activator of EIF4G3 (By similarity). May also involved in behavior (PubMed:24726472).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR and has multiple differences in the coding region, compared to variant 1. The resulting protein (isoform 4) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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