Sox17 (NM_001289467) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226835 representing NM_001289467 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGACCACCCCAACTACAAGTACCGGCCGCGGCGGCGCAAGCAGGTGAAGCGCATGAAGCGGGTGG AGGGAGGCTTCCTGCACGCTCTCGTCGAGCCCCAGGCCGGCGCGCTTGGTCCCGAGGGCGGCCGCGTGGC CATGGATGGCCTGGGTCTGCCTTTCCCGGAGCCGGGCTATCCGGCCGGTCCTCCGCTGATGTCTCCGCAC ATGGGCCCCCACTATCGGGACTGCCAGGGACTGGGCGCTCCCGCGCTCGACGGCTACCCTCTGCCCACTC CGGACACATCCCCGCTGGATGGCGTGGAGCAGGACCCGGCTTTCTTTGCAGCCCCGCTGCCAGGGGACTG CCCGGCGGCCGGCACCTACACTTACGCTCCAGTCTCGGACTATGCAGTGTCCGTAGAGCCGCCCGCTGGC CCCATGCGAGTGGGGCCGGACCCCTCGGGCCCTGCGATGCCGGGGATCCTGGCGCCCCCCAGCGCTCTGC ACCTGTACTACGGCGCGATGGGCTCGCCCGCCGCAAGTGCGGGGCGCGGTTTCCACGCGCAACCCCAGCA GCCGCTGCAACCGCAGGCACCGCCGCCGCCACCGCAGCAGCAGCACCCAGCGCACGGCCCCGGGCAACCT TCGCCCCCTCCCGAGGCTCTGCCCTGCCGGGATGGCACGGAATCCAACCAGCCCACTGAGCTCCTAGGGG AGGTGGACCGCACGGAATTCGAACAGTATCTGCCCTTTGTGTATAAGCCCGAGATGGGTCTTCCCTACCA GGGACACGACTGCGGAGTGAACCTCTCAGACAGCCACGGAGCCATTTCCTCCGTGGTGTCCGACGCTAGC TCAGCGGTCTACTATTGCAACTACCCCGACATTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289467
Insert Size	876 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289467.1, NP_001276396.1
RefSeq Size	2897 bp
RefSeq ORF	876 bp
Locus ID	20671
UniProt ID	Q61473
Gene Summary	This gene encodes a member of the Sox (Sry-related high mobility group box) family of transcription factors involved in the regulation of embryonic development. The encoded protein plays a role in the determination of cell fate and in maintaining cell identity. This gene regulates tumor angiogenesis and tumor progression. Mutations in the human gene are associated with vesicoureteral reflux, characterized by the backward flow of urine from the bladder into the ureters or the kidney. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon compared to variant 1. The resulting isoform (c, also known as t-Sox17; PMID 8636240) has a shorter N-terminus compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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