4833439L19Rik (NM_001252646) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4930558H15Rik; C81457; Kiaa1191; P33monox
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226781 representing NM_001252646 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCTGCCCATCGGGATGTGCCGTCGGGCATTCAGCTATGATGATGCCCTAGAGGACCCTGCGCCCA TGACTCCTCCTCCATCGGACATGGGCAGCATCCCCTGGAAGCCAGTGATTCCAGAGCGCAAGTATCAGCA TCTTGACAAGACAGAGGAGGGCGCGGCCAGTGTCTCTTCCCTTGCTGTGACTCCATCACCAGCCACTGAC AGTTCAGACAAGGCCCCTGTGGTGAAGGCTAAAGCTACCCATGTCATCATGAGTTCCCTGATCACAAAGC AGACCCAGGAGAGCATCCAGCGTTTTGAACAACAGGCAGGACTGAGAGATGCTGGCTACACACCCCATAA GGGCCTTACCACGGAGGAGACCAAGTACCTTCGAGTGGCAGAAGCACTCCATAAACTAAAGCTGCAGAGT GGAGAGACAGCAAAAGAAGAGAAGCACCCTGCATCAGCCCAGTCCACCCCGAGCAGCACCCCTCACGCGT CCCCTAAGCAGAAGTCCAGAGGCTGGTTCCCATCTGGCTCCTCCACAGCCTTACCTGCTCCGAATCCTCA CACCATGGATCCTGGGAGTGGAAATGATAGGAACTCAGCAGACAAATGGAGCCTCTTTGGACCACGACCC CTGCAGAAATCTGATTCTGGTTTTGCTATTCAGGCTTACAAAGGTGCCCCAAGACCCTCTCCTATGGAGG TGATGCGTGCCCAGGCCACACGAGTAGGTGAAGATCCAGCAACTTTCAAACCACCAAAGATGGATGTCCC AATGGTAGAAGGAAAGAAGCAACCACTTCGAACCCATAATCTCAAACCCCGTGACCTGAATGTGCTCACA CCCACTGGCTTCTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001252646
Insert Size	855 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252646.1, NP_001239575.1
RefSeq Size	2494 bp
RefSeq ORF	855 bp
Locus ID	97820
UniProt ID	Q9DBN4
Gene Summary	Potential NADPH-dependent oxidoreductase. May be involved in the regulation of neuronal survival, differentiation and axonal outgrowth (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks two exons in the 5' region, which results in a downstream AUG start codon, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform.

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