Setmar (NM_001276356) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5830404F24Rik; Etet2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226721 representing NM_001276356 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAGGAGCATCTCTTTCATCCCTGTAGGACATCCCTGCACAGCTAAAGTGTATACTCCTGATCATG TTGCTGGACCTGGAGCAGACATTGACCCCACACAAATAACCTTCCCTGGATGTGCTTGCATCGAAACTCC ATGTGTCCCTGGCACTTGCTCTTGTCTTCGCCATGAGAATAACTACGATGACAATTTATGCCTTAGGGAT GTGGGATCCGAAGGAAAGTACGCCAAGCCAGTTTTTGAATGCAATGTTCTGTGCCAGTGTGGCATGCGCT GCAGAAATAGGGTGGTCCAGAATGGCCTACACTTCCTTCTCCAGGTGTTCCAGACAGAGAAAAAAGGCTG GGGACTTCGGACTTTGGAATTTATACCCAAGGGAAGATTTGTCTGTGAGTATGCTGGGGAGGTGTTAGGA TTCTCTGAAGTGCAAAGAAGAATTCACCTACAAACATCACATGACTCAAATTACATCATCGCTGTCAGAG AACACATTTACAGTGGACAGATCATGGAAACATTTGTCGACCCTACCTACATAGGAAATATTGGGAGATT CCTCAACCATTCTTGTGAACCAAATCTGTTGATGATTCCTGTCCGAATTGACTCAATGGTACCCAAGCTG GCACTTTTCGCAGCCAAAGATATTTTGCCAGGAGAAGAACTCTCTTACGACTATTCAGGAAGATTCCTTA ACCAAGTAAGTAGTAAAGACAAAGAAAAGATAGACTGTAGCCCACCACGAAAGCCTTGTTACTGTGGGGC CCAGTCATGCACCACTTTCCTGCCCTATGACAGTTCACTATATATGGCCCCTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276356
Insert Size	825 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276356.1, NP_001263285.1
RefSeq Size	1770 bp
RefSeq ORF	825 bp
Locus ID	74729
Gene Summary	This gene encodes a histone-lysine N-methyltransferase that may be involved in the methylation of histone H3. In anthropoid primates this gene is a fusion gene of a SET histone-lysine N-methyltransferase and a mariner (MAR) family transposase. In all other species this gene contains only the SET domain. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (2) uses an alternate internal exon, compared to variant 1. This difference results in a distinct 5' UTR and causes translation initiation at an alternate start codon, compared to variant 1. The encoded protein (isoform 2) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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