Taf9 (NM_001015889) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2310012M09Rik; AA673500; AU045423; Taf2g; TAFII31; TAFII32
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226664 representing NM_001015889 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCTGGCAAGATGGCGTCTCCCAAGAGCATGCCGAAAGATGCACAGATGATGGCACAAATCCTGA AGGATATGGGGATTACAGAATATGAGCCAAGAGTTATAAATCAGATGCTGGAGTTTGCCTTCCGATATGT GACAACAATTCTAGATGATGCTAAAATTTATTCCAGCCATGCTAAGAAAGCTACTGTTGATGCAGATGAC GTACGACTGGCAATCCAGTGCCGTGCTGACCAGTCTTTCACTTCTCCACCCCCGAGAGATTTCTTATTAG ATATTGCACGACAAAGAAATCAAACCCCTTTGCCACTGATCAAGCCATATTCAGGTCCTAGATTGCCACC TGATAGGTATTGCTTAACTGCCCCAAACTATAGGCTTAAGTCTTTACAGAAAAAGGCACCTGCTCCTGCA GGAAGAATAACAGTTCCAAGGTTAAGTGTTGGTTCAGTTTCTAGTAGACCAAGTACTCCCACCCTAGGTA CACCGACTCCACAAACCATGTCTGTTTCAACTAAAGTAGGCACCCCAATGTCCCTCACAGGACAGAGGTT TACAGTACAGATGCCTGCTTCTCAGTCCCCTGCTGTAAAAGCTTCTATACCTGCAACATCAACAGTTCAG AATGTTCTTATTAATCCATCGTTAATTGGGTCCAAAAACATTCTTATTACTACTAATATGGTATCACAGA ATACAGCCGAGTCAGCAAATGCACTGAAACGGAAACGTGAAGACGATGATGATGACGATGATGACGATGA TGATGATGACTATGATAATATGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001015889
Insert Size	795 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001015889.2, NP_001015889.1
RefSeq Size	1248 bp
RefSeq ORF	795 bp
Locus ID	108143
UniProt ID	Q8VI33
Gene Summary	Essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) represents the longer transcript. Variants 1 and 3 encode the same protein.

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