Cpa6 (NM_001289497) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9030616D13Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226649 representing NM_001289497 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCTGGGCAGAAAATCACGAGCCTACAAAAGAGCTGTCTGGATAGACTGCGGCATTCACGCAAGAG AATGGATCGGTCCTGCCTTTTGTCAGTGGTTTGTAAGAGAAGCCATTCTGACCTACAAGACTGACCCAGC CATGAAAAAAATGTTGAATCATCTGTATTTCTATATCATGCCTGTATTCAATGTCGATGGATACCATTTC AGCTGGACACATGATAGATTCTGGAGAAAGACCCGGTCAAGAGACTCTAAGTTTCGCTGTCGTGGAGTTG ATGCCAATCGCAACTGGAAAGTGAAATGGTGCGATGAAGGAGCCTCCGCACACCCTTGTGATGATACATA CTGTGGTCCTTTCCCAGAATCTGAACCAGAAGTGAAGGCTGTAGCTAACTTCCTTCGAAAACACAGAAAG CGCATCAGGGCGTACCTGTCTTTTCATGCCTATGCTCAGATGTTGCTATATCCTTATTCTTACAAATATG CAACAATCCCCAATTTTAGCTGTGTGGAATTTGCAGCTCACAAAGCTGTGAAGGCACTTCGGTCGGTACA TGGGATCCGATACAGACATGGCCCCGCCTCCCAAACACTGTATGTAAGCTCTGGTAACTCAATGGATTGG GCTTATAAAAATGGAATACCCTATGCATTTGCTTTTGAACTGCGAGACACTGGGCATTTTGGATTTCTAC TGCCAGAGATGCTCATTAAACCCACCTGCACAGAGACCATGCTGGCTGTGAAGAATATCACAATGCACCT GCTAAAGAAATGTCCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289497
Insert Size	789 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289497.1, NP_001276426.1
RefSeq Size	1815 bp
RefSeq ORF	789 bp
Locus ID	329093
UniProt ID	Q5U901
Gene Summary	This gene encodes a protein that belongs to the metallocarboxypeptidase family of proteins that catalyze the release of a C-terminal amino acid from the target protein. The encoded preproprotein undergoes proteolytic cleavage to yield the mature form which is thought to play a role in cell migration. In humans, this protein regulates neuropeptides in the brain and mutations in this gene are associated with a recessive familial form of febrile seizures and with temporal lobe epilepsy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (2) differs in the 5' UTR, lacks an in-frame portion of the 5' coding region and initiates translation at a downstream start codon compared to variant 1. The resulting isoform (2) has a shorter N-terminus compared to isoform 1.

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