Ldhb (NM_001302765) Mouse Untagged Clone
CAT#: MC226635
Ldhb (untagged) - Mouse lactate dehydrogenase B (Ldhb), transcript variant 2
CNY 2,570.00
货期*
3周
规格
Product images
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经常一起买 (4)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | AI790582; H-Ld; H-Ldh; Ldh-; Ldh-2; LDH-B; LDH-H; Ldh2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC226635 representing NM_001302765
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAACCCTTAAGGAGAAGCTCATTGCGTCCGTTGCAGATGATGAGGCTGCCGTCCCGAACAACAAGA TCACTGTAGTGGGCGTTGGACAAGTGGGTATGGCATGTGCCATCAGCATTCTGGGAAAGTCTCTGGCTGA TGAACTTGCCCTGGTGGATGTGTTGGAAGACAAGCTCAAAGGAGAGATGATGGACCTGCAGCACGGGAGC TTGTTCCTCCAGACTCCGAAAATTGTGGCCGATAAAGATTACTCTGTGACAGCCAACTCTAAGATTGTGG TGGTGACGGCAGGAGTCCGCCAGCAGGAGGGGGAGAGTCGGCTCAACCTGGTGCAGAGAAATGTCAACGT GTTCAAGTTCATCATTCCTCAGATCGTCAAGTACAGCCCTGACTGCACCATCATCGTGGTTTCCAACCCA GTGGATATTCTGACTTACGTCACCTGGAAACTGAGCGGGCTACCTAAGCACCGTGTGATTGGAAGCGGAT GCAATCTGGATTCTGCTCGATTCCGCTACCTCATGGCAGAGAAGCTTGGCATTCATCCCAGCAGCTGCCA CGGATGGATCCTGGGCGAGCATGGAGACTCCAGTGTGGCTGTGTGGAGCGGGGTGAATGTGGCAGGAGTC TCCCTCCAGGAACTGAATCCAGAAATGGGGACAGACAATGACAGTGAGAACTGGAAGGAGGTGCATAAGA TGGTGGTGGACAGGGAATGTACGGCATTGAGAATGAAGTCTTCCTCAGTCTCCCGTGCATCCTCAATGCT CGGGGGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001302765 |
Insert Size | 780 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001302765.1, NP_001289694.1 |
RefSeq Size | 1214 bp |
RefSeq ORF | 780 bp |
Locus ID | 16832 |
UniProt ID | P16125 |
Gene Summary | This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have also been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes 1 and 19. [provided by RefSeq, Feb 2016] Transcript Variant: This variant (2) lacks an exon in the 3' coding region, which results in a frameshift and a different 3' UTR compared to variant 1. The encoded isoform (2) is shorter with a distinct C-terminus compared to isoform Ldhb. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR228846 | Ldhb (myc-DDK-tagged) - Mouse lactate dehydrogenase B (Ldhb), transcript variant 2 |
CNY 2,570.00 |
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