Snrpn (NM_013670) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2410045I01Rik; HCERN3; Peg; Peg4; Pwc; sm-D; SMN; snRNP-N
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226510 representing NM_013670 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGTGGGTAAGAGTAGCAAGATGCTGCAGCACATTGACTATAGGATGAGATGTATCCTGCAAGATG GGAGAATCTTCATTGGCACCTTCAAGGCTTTTGACAAGCATATGAATTTGATCCTCTGTGATTGTGATGA GTTCAGGAAGATCAAGCCAAAGAATGCAAAACAGCCAGAACGTGAAGAAAAACGGGTTTTGGGTCTGGTC TTGCTACGTGGGGAGAACTTGGTTTCAATGACTGTGGAGGGCCCACCTCCTAAAGATACTGGCATTGCTC GTGTGCCTCTTGCTGGCGCTGCAGGTGGCCCTGGGGTTGGAAGAGCAGCTGGCAGAGGAGTGCCAGCAGG TGTACCTATTCCCCAGGCTCCTGCTGGATTAGCAGGCCCTGTCAGAGGAGTTGGAGGCCCATCCCAGCAG GTCATGACCCCACAGGGAAGAGGCACTGTTGCAGCTGCTGCTGTTGCTGCTACTGCTAGCATTGCAGGAG CCCCAACCCAGTACCCGCCAGGACGGGGAACTCCACCTCCACCTGTAGGCAGAGCAACCCCACCTCCAGG CATTATGGCTCCTCCACCTGGTATGAGACCACCCATGGGCCCACCCATTGGGCTTCCCCCTGCTCGTGGG ACACCTATAGGCATGCCTCCTCCAGGAATGAGACCCCCTCCACCAGGAATTAGAGGCCCACCTCCCCCAG GAATGCGCCCACCAAGACCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_013670
Insert Size	723 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_013670.3, NP_038698.1
RefSeq Size	1970 bp
RefSeq ORF	723 bp
Locus ID	20646
UniProt ID	P63163
Gene Summary	This locus represents a paternally-expressed imprinted gene that encodes a component of the small nuclear ribonucleoprotein complex, which functions in pre-mRNA processing. Genomic and genetic changes in this region result in growth defects and lethality; the corresponding region in human is the critical region for Prader-Willi Syndrome. Alternative promoter use and alternative splicing result in a multitude of transcript variants encoding the same protein. Transcript variants may be bicistronic and also encode the SNRPN upstream reading frame protein (Snurf) from an upstream open reading frame. In addition, long spliced transcripts for small nucleolar RNA host gene 14 (Snhg14) may originate from the promoters at this locus and incorporate exons shared with this gene. [provided by RefSeq, Mar 2017] Transcript Variant: This variant (1) represents the predominant variant. This splice variant is bicistronic and can also encode the Snurf protein from an upstream open reading frame.

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