Gid8 (NM_001289651) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2310003C23Rik; 4833420G11Rik; AI451474; Twa1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226420 representing NM_001289651 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTATGCAGAAAAGCCGGATGAAATAACCAAAGATGAATGGATGGAAAAGCTCAATAACTTGCATG TTCAACGAGCAGACATGAACCGACTCATCATGAACTACCTAGTCACAGAGGGCTTTAAGGAAGCAGCAGA GAAATTTCGGATGGAGTCTGGGATCGAACCAAGTGTGGACCTAGAAACACTTGATGAGCGAATCAAGATT CGGGAGATGATTCTGAAGGGTCAGATCCAGGAGGCCATTGCCTTGATCAACAGCCTCCACCCAGAGCTCC TAGACACAAACCGGTATCTTTACTTCCACCTTCAGCAACAACACTTGATTGAGCTTATCCGTCAGCGTGA GACAGAAGCAGCATTGGAGTTTGCCCAGACACAACTGGCAGAGCAGGGTGAGGAAAGCCGAGAATGCCTC ACAGAGATGGAGCGCACACTGGCCTTGCTGGCCTTCGATAGCCCTGAGGAGTCGCCTTTTGGAGACCTCC TTCACATGATGCAGAGGCAAAAGGTATGGAGTGAAGTTAACCAGGCTGTTCTGGATTATGAGAATCGAGA GTCCACACCCAAGCTGGCAAAATTACTGAAACTGCTACTTTGGGCTCAGAATGAGCTAGACCAGAAGAAA GTAAAATATCCCAAAATGACAGACCTCAGCAAAGGTGTGATTGAGGAGCCCAAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289651
Insert Size	687 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289651.1, NP_001276580.1
RefSeq Size	4446 bp
RefSeq ORF	687 bp
Locus ID	76425
UniProt ID	Q9D7M1
Gene Summary	Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. Acts as a positive regulator of Wnt signaling pathway by promoting beta-catenin (CTNNB1) nuclear accumulation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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