Ndufs8 (NM_001271443) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	BC021616; CI-23kD; TYKY
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226326 representing NM_001271443 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATCGCCTGAGCTCATCAATGCTGCCACGGGCCTTGGCCCAGGCCATGCGCACAGGACATCTTAATG GACAAAGCCTTCATAGCAGCGCAGTGGCGGCAACGTACAAGTATGTGAATAAGAAGGAACAGGAGTCTGA GGTGGACATGAAGTCCGCAACTGACAATGCAGCTCGGATTCTGATGTGGACAGAACTCATCCGAGGACTG GGCATGACCCTAAGTTACCTCTTTCGAGAGCCTGCCACCATCAACTACCCCTTTGAGAAGGGCCCACTGA GTCCGCGCTTCCGTGGGGAGCATGCACTGCGCCGCTACCCATCTGGAGAGGAGCGTTGCATTGCCTGCAA ACTCTGTGAGGCCATCTGTCCTGCACAGGCCATCACCATTGAGGCTGAGCCAAGAGCCGATGGCAGCCGC CGAACGACACGCTATGACATCGACATGACCAAGTGTATCTACTGTGGTTTCTGCCAGGAAGCCTGCCCTG TTGATGCCATTGTGGAGGGCCCCAACTTCGAGTTCTCCACCGAGACACACGAGGAGTTGCTGTACAACAA GGAGAAGCTACTCAACAATGGAGACAAGTGGGAGGCCGAGATCGCTGCCAACATCCAGGCTGACTACCTG TACCGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271443
Insert Size	639 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271443.1, NP_001258372.1
RefSeq Size	814 bp
RefSeq ORF	639 bp
Locus ID	225887
UniProt ID	Q8K3J1
Gene Summary	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR, compared to variant 1. Variants 1, 2, and 3 encode the same protein.

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