Fgf12 (NM_001276419) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AV114868; B230343J05Rik; FGF-12; FHF-1; Fhf1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226300 representing NM_001276419 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGATTCTTGCGCTTTCTCTGAAGAAAGGAGTGCATATGCTGCAGTGCCTCTGTGGAAGGAGCCTGA GGTCCAGCGACAGCCCAAGCGAACCCCAGCTGAAAGGGATTGTGACAAGGTTATTCAGCCAGCAGGGATA TTTCCTGCAGATGCATCCAGATGGTACCATTGATGGGACCAAGGACGAAAACAGCGACTACACCCTCTTC AATCTAATTCCTGTAGGACTGCGTGTGGTGGCCATCCAAGGGGTTAAAGCCAGCCTTTATGTGGCCATGA ATGGAGAAGGCTATCTCTACAGCTCAGATGTTTTTACCCCAGAATGCAAATTTAAGGAGTCTGTGTTTGA AAACTACTATGTGATCTATTCCTCAACCCTGTATCGCCAGCAGGAATCAGGCCGTGCATGGTTTCTAGGA CTCAATAAAGAAGGTCAAATCATGAAGGGGAACAGAGTGAAGAAAACCAAGCCCTCATCTCATTTTGTAC CAAAACCTATTGAAGTGTGTATGTACAGAGAACCATCACTACACGAAATTGGAGAAAAGCAAGGACGCTC AAGGAAAAGTTCGGGAACACCCACCATGAATGGAGGCAAAGTCGTGAATCAAGATTCTACATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276419
Insert Size	624 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276419.2, NP_001263348.1
RefSeq Size	5544 bp
RefSeq ORF	624 bp
Locus ID	14167
UniProt ID	P61329
Gene Summary	Involved in nervous system development and function. Promote neuronal excitability by elevating the voltage dependence of neuronal sodium channel SCN8A fast inactivation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate 5' exon structure, differs in the 5' UTR and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform c which is shorter and has a distinct N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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