Acy3 (NM_001302482) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	0610006H10Rik; A; AA3; AAIII; Acy-3; AW107362; HCBP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226171 representing NM_001302482 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTCCCTACCTGGGTCCCGGGAGCCCCTGCTCCGTGTGGCTGTGACTGGAGGCACCCACGGGAATG AGATGTGTGGTGTCTACCTGGCCCGGTACTGGCTACAGAACCCAGGGGAGCTGCAGAGACCCAGCTTCTC AGCCATGCCGGTTCTGGCCAACCCAGCAGCCACAGCTGCCTGTTGCCGTTACCTGGACCGTGATCTCAAC CGCTCCTGCACCCTCACCTTCCTTGGCGGCAGAACCCGGGGATGCCCTGCCGCCTCTTTCTGTATGAGCC AGCTGGGACGGAGACCTTCAGCGTGGAATCTATATCCAAGAATGGAATCTGTCTGGAGATGGGCCCACAG CCTCAGGGCGTGCTGCGGGCCGACCTGTTCTCCCGGATGCGAGCTCTGGTGGCATCCATTCTGGACTTCA TCGAGCTCTTCAACCAAGGCATGGACTTACCCGCCTTTGAGATGGATATCTACAGGAACTTGGGCAGTGT GGACTTCCCACGCACTGCAGATGGTGACCTGGCTGGCACTGTGCACCCTCAACTGCAGGACCATGACTTT GAGCCACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302482
Insert Size	570 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302482.1, NP_001289411.1
RefSeq Size	994 bp
RefSeq ORF	570 bp
Locus ID	71670
UniProt ID	Q91XE4
Gene Summary	This gene encodes a member of the aminoacylase family of enzymes. This enzyme specifically deacetylates N-acetyl aromatic amino acids and mercapturic acids. Action of this enzyme on metabolites of the environmental contaminant trichloroethylene leads to the generation of toxic products that may lead to kidney failure. This protein has been found to bind to the hepatitis C virus core protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] Transcript Variant: This variant (4) differs in both UTRs and lacks an alternate exon in the 5' coding region compared to variant 1, resulting in a frameshift. The resulting protein (isoform 2) has a distinct C-terminus and is shorter than isoform 1.

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