Hikeshi (NM_001291287) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	0610007P06Rik; 1110002N09Rik; l(7)6Rn; l7Rn6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225976 representing NM_001291287 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGGAACAATCCCATTTCCTGAGGGCATGGGAGGATCTGTCTACTTTTCCTATCCTGATTCAAATG GGGTGCCAGTGTGGCAGCTCCTAGGATTTGTCACGAATGGAAAGCCAAGTGCCATCTTCAAAATATCAGG TCTTAAATCTGGGGAAGGAAGCCAGCACCCATTTGGAGCCATGAATATTGTGCGAACCCCATCTGTTGCC CAGATTGGCATCTCGGTGGAATTGTTGGACAGTCTGGCTCAGCAGACTCCTGTAGGCAGTGCTGCCGTGT CCTCCGTTGACTCCTTTACGCAGTTCACACAGAAGATGTTGGACAACTTCTACAATTTTGCTTCATCATT TGCTCTCTCTCAGGCCCAGATGACACCAAATCCATCAGAAATGTTCATCCCAGCAAATGTGGTTCTGAAA TGGTATGAAAACTTTCAAAGACGACTAGCACAGAACCCCCTCTTTTGGAAAACATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291287
Insert Size	477 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291287.1, NP_001278216.1
RefSeq Size	1149 bp
RefSeq ORF	477 bp
Locus ID	67669
UniProt ID	Q9DD02
Gene Summary	Acts as a specific nuclear import carrier for HSP70 proteins following heat-shock stress: acts by mediating the nucleoporin-dependent translocation of ATP-bound HSP70 proteins into the nucleus. HSP70 proteins import is required to protect cells from heat shock damages. Does not translocate ADP-bound HSP70 proteins into the nucleus (By similarity). May also be indirectly required for organization and/or function of the secretory apparatus in Clara cells in lung.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) contains an alternate exon and initiates translation at an alternate start codon compared to variant 1. The resulting isoform (c) is shorter at the N-terminus compared to isoform a.

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