Rbm3 (NM_001293658) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2600016C11Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225953 representing NM_001293658 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTCTGAAGAAGGGAAACTCTTCGTAGGAGGGCTCAACTTCAACACCGATGAACAGGCACTTGAAG ACCACTTCAGCAGCTTTGGGCCTATCTCTGAGGTGGTTGTTGTCAAGGACCGGGAGACTCAAAGATCCCG GGGTTTTGGCTTCATCACCTTCACAAACCCAGAGCATGCCTCAGATGCGATGAGAGCTATGAATGGAGAG TCCCTGGATGGGCGCCAAATCCGAGTTGATCATGCAGGAAAGTCTGCCAGGGGATCCAGAGGGGGTGCCT TTGGTGGGCGTGGTCGCAGTTACTCTAGAGGTGGTGGAGACCAGGGATATGGAAGTGGAAGATATGACAG TCGTCCTGGAGGATATGGATATGGGTATGGGCGGTCTAGAGACTACAGTGGCAGAAGCCAGGGTGGCTAT GACCGCTACTCAGGAGGAAATTACAGAGACAATTATGACAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001293658
Insert Size	465 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001293658.1, NP_001280587.1
RefSeq Size	1344 bp
RefSeq ORF	465 bp
Locus ID	19652
Gene Summary	Cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphorylation of translation initiation factors and active polysome formation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) contains an alternate terminal exon in the 3' UTR, compared to variant 1. Variants 1, 2, and 5 encode the same protein (isoform 1).

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