Aoah (NM_001281854) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4930433E13Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225906 representing NM_001281854 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTTTCCCTGGAAAGTCTTCAAGACCACCCTTCTGCTTCTTCTGCTCTCTCACTCATTGGCCTCTG TGCCCTCTGAGGACCAGCCAGGTGACAGCTACTCCCATGGCCAAAGCTGTTTAGGGTGTGTGGTACTGGT ATCTGTGATTGAGCAACTGGCTGAAGTTCACAACTCTTCAGTCCAGGTGGCGATGGAGAGACTCTGCAGC TACCTGCCTGAAAAGCTGTTCTTGAAGACCGCCTGCTATTTCTTGGTTCAGACATTTGGCTCAGACATCA TAAAACTACTCGATGAAGCTATGAAGGCTGATGTGGTGTGCTATGCTCTGGAATTTTGCAAGCGGGGTGC TGTCCAACCACAGTGTCATCTCTACCCACTTCCCCAGGACAACCAACTCATCTCAAGATGTCCAGAACGT TTCAGGGATCAGTACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001281854
Insert Size	438 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001281854.1, NP_001268783.1
RefSeq Size	936 bp
RefSeq ORF	438 bp
Locus ID	27052
UniProt ID	O35298
Gene Summary	This genes encodes an enzyme that catalyzes the hydrolysis of acyloxylacyl-linked fatty acyl chains from bacterial lipopolysaccharides. The encoded protein modulates host inflammatory response to gram-negative bacteria. The proprotein is further cleaved into a large and small chain that interact in a heterodimer. Alternative splicing results in multiple transcript variants for this gene. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (2) lacks multiple 3' coding exons and contains an alternate 3' exon, resulting in a distinct 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1.

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