Tafa2 (NM_001252387) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6330575M02; Fam19; Fam19a2; FAM19A2 TAFA-2; Sa; Sam2; Taf; Tafa; Tafa-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225803 representing NM_001252387 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATAAGAGATACTTGCAGAAAGCAACACAAGGAAAGCTTCTGATAATTATTTTTATAGTGACCTTGT GGGGGAAAGCCGTTTCCAGCGCCAACCATCACAAAGCTCACCATGTTAGAACTGGGACTTGCGAGGTTGT GGCGCTGCACAGATGCTGTAATAAGAACAAGATAGAAGAACGGTCCCAAACGGTCAAGTGCTCCTGCTTC CCTGGGCAGGTGGCAGGCACTACCCGAGCTGCTCCGTCTTGTGTGGATGCATCCATAGTGGAACAAAAGT GGTGGTGTCATATGCAGCCATGCCTGGAGGGAGAGGAATGTAAAGTCCTTCCAGATCGCAAAGGATGGAG CTGTTCCTCTGGAAACAAAGTAAAAACAACTAGGGTAACCCATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252387
Insert Size	396 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252387.1, NP_001239316.1
RefSeq Size	4152 bp
RefSeq ORF	396 bp
Locus ID	268354
UniProt ID	Q7TPG7
Gene Summary	This gene is a member of the TAFA family which is composed of five highly homologous genes that encode small secreted proteins. These proteins contain conserved cysteine residues at fixed positions, and are distantly related to MIP-1alpha, a member of the CC-chemokine family. The TAFA proteins are predominantly expressed in specific regions of the brain, and are postulated to function as brain-specific chemokines or neurokines that act as regulators of immune and nervous cells. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) lacks an exon and uses a downstream, in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1.

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