Lama3 (NM_010680) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Lama3B; [a]3B
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225478 representing NM_010680 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTGGCTCTGGGCAGGGCACCGCGATCCTTACCGCTGCTGCTCACTCTATTGCTGCTGCTGTTGC TGAGGATGTCGCCCAGCTGGAGCGTGGTGGGTCAGGACCATCCCATGTCCTCGCGCAGCCTGCACCCGCC CTACTTCAACCTGGCCCAGGCGGCGAGAATCTGGGCCACCGCCACCTGCGGGGAGCGGGACCCAGAGGTC TCAAGGCCCCGGCCTGAGCTTTTCTGCAAATTGGTGGGCGGCCCCGCTGCACAGGGCAGCGGCCACACCA TCCAGGGCCAGTTCTGTGACTACTGCAATTCTGAGGATTCCAGGAAGGCACATCCAGCCAGCCATGCAAT TGATGGATCGGAACGGTGGTGGCAAAGCCCACCTCTCTCCTCCGGCACACAGTACAATCAAGTCAACCTC ACCTTGGATCTGGGTCAGCTCTTCCATGTGGCTTATATCTTAATCAAATTTGCAAACTCTCCCCGCCCTG ATCTTTGGATCCTGGAAAGATCTGTAGACTTTGGAAGCACCTACTCACCATGGCAGTATTTTGCTCATTC TAGAAGAGATTGTGTAGAACAGTTTGGGCAAGAAGCAAACATGGCAATTACCCAGGACGACCAGATGCTC TGTGTCACGGAGTATTCCCGTATCGTGCCTCTGGAAAATGGCGAGATTGTTGTATCCTTGATAAATGGTC GTCCAGGTGCAAAAAAGTTTGCTTTCTCTGACACTCTGAGGGAGTTTACTAAGGCAACAAACATCCGCTT GCGGTTTCTGCGAACCAACACCCTCCTCGGGCATCTTATTTCCAAGGCAGAGCGAGACCCCACTGTCACG CGCCGGTATTATTACAGCATAAAGGATATCAGTGTTGGTGGGCGGTGTGTTTGCAACGGCCATGCGGAGG CGTGCAGTGCTGACAACCCTGAAAAGCAGTTCCGATGCGAATGCCAGCACCATACCTGTGGAGACACGTG TAACCGCTGCTGTGCAGGTTACAATCAGAGGCGCTGGCAGCCTGCTGGTCAGGAGCAGCACAATGAGTGT GAAGCCTGCAACTGCCATGGGCATGCTGTGGACTGCTACTATGACCCAGACGTGGAGCACCAGCAGGCGA GCTTGAACAGCAAAGGCGTCTACGCAGGTGGAGGGGTCTGCATCAACTGTCAGCACAACACTGCAGGCGT GAACTGTGAAAAGTGTGCGAAGGGTTACTTCCGGCCCCATGGAGTTCCGGTGGATGCACTGCATGGATGC ATCCCTTGCAGCTGTGACCCAGAACGCGCAGATGACTGTGACCAGGGCTCAGGCCACTGCCATTGTAAGC CAAATTTCTCCGGAGACTACTGTGAGACGTGTGCAGATGGGTACTATAATTTTCCATTTTGCTTGAGAAT TCCAGTCTTTCCCAACTACACTCCAAGTCCAGAAGATCCAGTGGCTGGCAATATAAAAGGGTGTGACTGT AACTTGGAAGGTGTTCTCCCAGAGATATGTGACGATCGTGGCAGGTGCCTGTGCCGCCCTGGGGTTGAGG GTCCCCAGTGTGACTCCTGCCGCTCGGGCTCCTATTCATTTCCCATATGCCAAGCTTGCCAGTGTTCGAC GATTGGATCCTATCCAGTGCCCTGTGACCCGGGGAATGGCCAGTGTGACTGCCTGCCTGGAATTACCGGG AGGCAGTGTGACAGGTGTCTCTCGGGAGCCTATGACTTTCCATACTGCCAAGGGTCCGGCAGTGTTTGCC ATCCAGCTGGGACCCTTGACTCCAGCCTGGGTTATTGTCAGTGCAAGCAGCATGTTGCAAGTCCTACATG TAGTGTCTGCAAACCATTATATTGGAATCTGGCCAAAGAAAACCCCCGTGGATGCTCAGAGTGCCAGTGC CATGAAGCAGGGACATTGAGTGGAATTGGAGAGTGTGGGCAGGAGGACGGTGACTGTAGCTGCAAAGCCC ATGTAACTGGTGATGCCTGCGACACCTGTGAAGATGGGTTTTTCTCTTTGGAGAAGAGCAATTACTTTGG CTGTCAAGGGTGTCAGTGTGACATTGGTGGAGCACTCACCACCATGTGTAGTGGGCCCTCGGGAGTATGC CAGTGCAGAGAGCACGTGGAGGGGAAACAGTGCCAGAGGCCTGAAAATAACTACTACTTCCCGGATTTGC ACCACATGAAGTATGAGGTCGAAGATGGCACTGGACCTAATGGAAGAAACCTGCGGTTTGGATTTGATCC CCTGGTATTCCCTGAGTTTAGCTGGAGAGGATATGCTCCAATGACCTCAGTCCAGAATGAAGTAAGGGTG AGATTGTCTGTGAGGCAGTCCAGCCTCTCCTTGTTCCGCATCGTTCTGAGATACATCAGTCCTGGAACGG AAGCCATATCCGGCCGAATCACTCTTTACTCATCGCAGGGAGATTCGGATGCTTTGCAAAGCAGAAAAAT CACCTTTCCCCCGAGTAAAGAGCCAGCCTTTGTCACAGTCCCTGGGAATGGCTTTGCAGGCCCATTCTCC ATCACACCTGGGACGTGGATTGCTTGCATCCAGGTGGAAGGAGTCCTTCTGGACTACCTGGTGCTGCTTC CCAGGGACTACTATGAAGCATTCACCCTGCAAGTGCCAGTCACAGAGCCATGTGCCCACACAGGATCTCC CCAGGACAACTGTTTGCTTTACCAGCATTTACCACTGACTGCATTCTCCTGTACCCTGGCTTGTGAGGCC AGACACTTCCTGCTGGATGGAGAGCTGAGACCCTTGGCAATGAGGCAGCCCACACCCACACACCCAGCCA TGGTGGACCTCAGCGGGAGAGAGGTAGAACTGCAGCTTCGTCTGCGGGTCCCACAGGTTGGCCACTACGT GGTCCTGCTGGAGTATGCCACGGAGGTGGAGCAGCTTTTTGTGGTGGACGTGAATCTGAAGAGCTCAGGG TCTGCCTTGGCAGGCCAGGTGAACATATACAGCTGCAAGTACAGCATCCCGTGCAGGAGTGTGGTGATTG ACAGCCTGAGTCGCACGGCTGTACATGAGCTGTTGGCAGATGCAGACATTCAGCTCAAGGCGCACATGGC CCATTTCCTTTTGTATCACATTTGTATTATACCAGCTGAAGAATTCTCAACTGAATATTTGAGACCTCAA GTCCACTGCATTGCCAGCTACAGGCAGCATGCTAATCCAAGTGCTTCCTGTGTCTCCCTGGCCCATGAAA CTCCTCCAACAGCCTCAATTTTGGATGCTACAAGTAGGGGCCTTTTCTCTGCCCTACCTCATGAGCCTTC CTCTCCTGCAGATGGAGTTACTCTGAAGGCACCACAGAGTCAAGTGACCCTGAAAGGACTCATACCACAC CTGGGCCGACACGTCTTTGTCATCCATTTTTATCAAGCAGAGCACCCAGGGTTTCCCACTGAGGTGATTG TGAATGGAGGAAGACAGTGGTCAGGTTCCTTCCTTGCCTCCTTCTGTCCCCACTTACTTGGCTGCCGGGA CCAGGTGATCTCTGATGGCCAAGTGGAGTTTGACATCTCTGAAGCAGAGGTAGCTGTGACAGTGAAGATT CCAGATGGAAAGTCCTTAACATTGGTCCGGGTTCTAGTGGTACCTGCAGAGAATTACGACTACCAAATTC TTCACAAAACAACAGTGGACAAGTCCTCCGAGTTCATCAGCAGTTGTGGAGGAGACAGCTTTTATATTGA TCCCCAGGCAGCCTCTGGATTCTGTAAGAATTCTGCAAGGTCCCTGGTAGCCTTTTACCATAACGGTGCC ATACCCTGTGAGTGCGACCCTGCTGGGACTGCCGGCCACCACTGTAGTCCTGAGGGTGGGCAGTGCCCTT GCCGGCCCAATGTCATCGGGAGGCAGTGCAGCCGCTGTGCGACAGGCTACTATGGATTCCCATACTGCAA GCCTTGTAATTGTGGCAGACGCCTTTGTGAAGAGGTGACAGGGAAGTGTCTCTGCCCACCCCACACAGTC AGGCCTCAGTGTGAGGTCTGTGAGATGAATTCCTTCAACTTTCACCCTGTGGCTGGCTGTGACGTCTGCA ACTGCTCCAGGAAGGGCACCATTGAGGCGGCCGTCTCTGAGTGTGACAGGGACAGCGGGCAGTGCAGGTG CAAGCCTAGAGTCACAGGGCAGCAGTGTGACAAGTGTGCTCCTGGCTTCTACCAGTTCCCTGAGTGTGTC CCCTGCAGCTGTAACAGAGATGGGACTGAGCCCAGCGTATGTGACCCAGAGACTGGGGCTTGCATGTGCA AGGAAAATGTAGAGGGCCCCCAATGTCAACTGTGTCGAGAAGGATCATTCTACCTGGACCCAACAAACCC AAAGGGTTGTACCAAGTGCTTCTGTTTTGGAGTGAATACTGACTGTCAGAGTTCGCATAAGCAACGAGCT AAGTTTGTAGACATGATGGGCTGGCGTCTGGAGACAGCAGATGGAGTTGATGTCCCTGTGTCCTTCAACC CTGGCAGCAACAGCATGGTGGCAGATCTGCAGGAGCTGCCACCCTCAGTTCACAGTGCATCCTGGGTGGC ACCTCCATCCTACCTAGGTGATAAGGTATCATCGTACGGCGGCTACCTCACCTACCACGCCAAGTCCTTT GGCTTACCTGGAGATATGGTTCTTCTGGGAAAGCAGCCAGATGTGCAGCTCACTGGTCAACACATGTCCC TCATCCATAAGGAACCCAGCGACCCACGGCCAGACAGGCTGCATCACGGAAGAGTGCAAGTGATTGAGGG AAACTTCAGACACGAAGGCAGCAGTGCCCCAGTGTCCCGGGAGGAGCTGATGACTGTGCTGTCCAGACTG GAAAGACTCCACATCCGGGGCCTCCATTTCACCGAGACACAGCGGCTCACCTTGGGTGAGGTAGGGCTGG AGGAGGCCTCTGACACGGGAAGCGGACCCAGGGCTCATCTTGTGGAGATGTGTGCCTGCCCCCCTGACTA CACAGGTGACTCATGCCAGGGTTGTCGCCCTGGATACTATTGGGACAACAAAAGCTTACCTGTAGGAAGG TGTGTTCCCTGCAATTGCAACGGACATTCAAATAGATGCCAGGATGGCTCCGGGATATGCATTAACTGTC AGCACAACACAGCTGGGGAGCACTGTGAGCGTTGCCAAGCAGGTCACTATGGAAATGCCATCCACGGATC TTGTAGGGTCTGCCCCTGCCCTCATACCAACAGTTTTGCCACCGGCTGTGCTGTGGATGGTGGAGCTGTG AGGTGTGCCTGCAAACCCGGATACACAGGAACACAGTGTGAGAGGTGTGCACCAGGATATTTTGGGAACC CCCAGAAATTTGGAGGTAGCTGCCAGCCATGCAATTGTAACAGCAATGGCCAGTTAGGTCCTTGCGACCC CCTAACTGGAGACTGTGTAAACCAAGAACCCAAAGATGGCAGCCCTGCAGAAGAATGTGATGACTGCGAC AGCTGTGTGATGACGCTCTTAAATGACTTGGCCTCCATGGGTGAGGAACTCCGCCTGGTGAAGTCAAAGC TGCAGGGGCTGAGTGTGAGCACGGGTGCTCTGGAACAGATCCGGCACATGGAGACGCAGGCCAAGGACCT GAGGAACCAGCTGCTTGGCTTCCGTTCTGCCACCTCAAGTCATGGGTCCAAAATGGATGACCTGGAAAAA GAGCTGAGTCATTTGAACCGGGAATTTGAAACTCTGCAAGAAAAGGCACAGGTCAATTCCAGAAAAGCAC AAACATTATATAACAACATTGATCAGACAATCCAAAGTGCCAAAGAACTGGACATGAAGATTAAAAACAT CGTTCAGAATGTGCACATTCTCCTGAAGCAGATGGCGAGGCCAGGTGGAGAAGGCACGGACTTGCCAGTG GGTGACTGGTCCAGGGAGCTGGCCGAAGCTCAACGCATGATGCGAGACCTGCGAAGCCGAGACTTTAAAA AGCACCTCCAAGAAGCAGAGGCCGAGAAAATGGAAGCCCAGCTCTTACTGCACCGGATCAGGACCTGGCT GGAATCCCACCAGGTGGAGAACAACGGACTGCTAAAGAATATTCGGGACTCCTTAAATGATTATGAAGAC AAACTTCAGGACCTACGTTCCATCCTCCAGGAGGCAGCTGCCCAGGCAAAGCAGGCCACTGGCATCAACC ATGAAAATGAGGGGGTTCTCGGAGCCATCCAGAGACAAATGAAAGAAATGGATTCCCTGAAGAATGACTT CACCAAGTACCTGGCCACAGCCGACTCTTCCCTGCTGCAGACCAACAATCTACTGCAGCAGATGGACAAA AGCCAGAAGGAATATGAAAGCTTAGCTGCTGCTTTAAATGGAGCAAGACAGGAACTGAGTGACAGAGTGC GAGAACTGTCCAGATCGGGTGGCAAAGCACCGCTGGTGGTGGAGGCAGAGAAGCATGCACAGTCTTTACA GGAGCTGGCAAAGCAGCTGGAAGAGATAAAGAGAAACACCAGCGGGGATGAGCTGGTGCGTTGTGCTGTG GATGCTGCCACGGCCTATGAGAACATCCTCAATGCCATCAGAGCAGCAGAGGATGCAGCCAGCAAGGCCA CCAGTGCCTCCAAGTCTGCCTTCCAAACAGTGATAAAGGAAGACCTTCCAAAAAGAGCTAAGACCCTGAG TTCTGACAGCGAGGAACTGTTAAATGAAGCCAAGATGACACAGAAAAGGCTACAGCAAGTCAGTCCAGCT CTCAACAGCCTACAACAAACCCTGAAGACTGTATCAGTTCAGAAGGACCTGCTAGATGCCAACCTCACTG TTGCCCGTGATGATCTTCATGGGATACAGAGAGGTGATATCGACAGTGTGGTGATCGGTGCAAAGAGCAT GGTCAGGGAAGCCAACGGAATAACAAGCGAGGTCCTGGACGGGCTCAACCCCATCCAGACAGATTTGGGA AGGATTAAGGACAGCTATGAGAGCGCACGGCGTGAAGACTTCAGCAAGGCTCTGGTCGATGCCAATAACT CAGTAAAGAAATTAACCAGGAAGTTGCCTGATCTTTTTATCAAGATTGAAAGTATCAACCAACAGTTGCT GCCCCTGGGGAACATCTCTGACAATGTGGACCGAATCCGAGAACTCATTCAGCAGGCCAGAGATGCTGCA AACAAGGTTGCAATTCCCATGAGGTTCAATGGTAAATCTGGTGTCGAAGTCCGACTGCCGAATGACCTAG AAGATTTAAAAGGATACACATCTCTGTCTTTGTTTCTCCAAAGACCTGACTTGCGAGAGAACGGAGGCAC TGAGGATATGTTTGTAATGTACCTTGGGAATAAGGATGCCTCCAAGGACTACATTGGCATGGCGGTTGTA GATGGCCAGCTGACATGTGTGTACAACCTGGGGGACCGAGAGGCTGAAGTTCAGATAGACCAGGTCCTGA CGGAGAGTGAGTCTCAGGAGGCAGTTATGGACCGGGTGAAGTTCCAGAGGATATATCAATTTGCCAAGCT TAATTACACCAAAGAAGCCACATCTACTAAACCCAAAGCCCCTGGGGTCTATGACATGGAGAGCGCCAGT AGCAACACACTCCTTAATTTGGATCCTGAGAATGCAGTATTTTACGTCGGCGGTTATCCACCTGGTTTTG AGCTTCCACGCAGACTGCGGTTCCCTCCATACAAAGGCTGTATCGAACTAGATGACCTCAACGAAAACGT TCTAAGCTTGTACAATTTCAAGACAACTTTCAATCTCAATACCACTGAAGTAGAGCCTTGTAGGAGGAGA AAGGAAGAGTCGGACAAAAATTACTTTGAAGGCACAGGCTATGCTCGAATTCCTACCCAGCCAAATGCTC CCTTCCCAAACTTCATGCAGACCATCCAAACTACTGTGGACAGAGGTCTACTGTTCTTCGCAGAAAACCA GGATAACTTCATATCTCTGAATATAGAAGACGGCAATCTCATGGTAAAATACAAACTAAATTCAGAGCCA CCCAAAGAGAAAGGAATTCGAGACACCATCAACAACGGGAGAGATCACATGATTTTAATCTCAATTGGAA AATCACAAAAGCGGATGTTGATAAATATGAATAAACATAGTATAATAATTGAAGGGGAAATATTTGATTT CAGCACATATTACTTGGGAGGAATTCCAATTGCAATCAGAGAAAGGTTTAACATCTCAACGCCTGCTTTC CAAGGCTGCATGAAGAATCTGAAGAAAACCAGTGGGGTTGTCAGGTTGAACGATACAGTGGGTGTGACCA AAAAGTGCTCAGAAGACTGGAAGCTTGTGCGAACCGCCTCGTTCTCCAGAGGAGGACAGATGAGTTTCAC AAACTTGGATGTGCCCTCACTTGACCGCTTCCAGCTCTCCTTTGGGTTCCAGACCTTTCAACCCAGCGGT ACACTACTAAACCATCAGACACGGACAAGTAGCCTACTGGTCACCCTGGAAGATGGTCATATTGCGTTGA GCACCAGGGACAGCAGCAGCCCGATTTTCAAGTCTCCAGGGACCTACATGGATGGTTTACTGCATCATGT ATCTGTAATAAGCGACACCTCAGGCCTGCGCCTTCTCATCGATGACCAGGTTCTGAGAAGAAACCAAAGG CTCGCTAGCTTCTCTAATGCCCAGCAGTCGCTCAGCATGGGAGGCGGTTATTTCGAGGGTTGTATCAGCA ACGTTTTTGTCCAAAGGATGTCACAGAGTCCAGAAGTCCTGGATATGGCCAGCAAGTCTACTAAGAGGGA TGCATTCCTAGGAGGCTGCAGTTTAAACAAGCCACCTTTTCTTATGTTGTTTAAGAGTCCCAAGGGATTT AACAAGGCCCGGAGTTTCAATGTCAATCAGCTGTTGCAAGATGCACCTCAGGCTGCAAGGAGCATAGAGG CTTGGCAAGATGGGAAGTCCTGCCTACCACCTCTGAACACCAAGGCCACTCACAGAGCCCTGCAGTTTGG GGACAGTCCTACCAGCCACTTGCTATTCAAGCTTCCCCAGGAGCTGCTGAAACCCAGGTTACAGTTTTCT TTGGACATACAGACAACTTCCTCCAGAGGGCTAGTGTTTCACACAGGCACCAGGGACTCCTTTGTGGCTC TCTATCTCTCAGAAGGCCATGTCATCTTTGCCTTGGGGGCAGGAGGGAAGAAACTGAGACTCAGAAGCAA AGAGAGATACCACGATGGGAAGTGGCACTCGGTGGTGTTTGGACTGAGTGGAAGAAAGGTGCACCTGGTG GTGGATGGGCTGAGGGCCCAGGAAGGCAGTTTGCCTGGAAACTCTACCATCAGCCCCAGAGAACAGGTTT ACCTGGGGTTGTCACCATCAAGAAAGTCAAAGAGCCTCCCCCAGCACAGTTTTGTGGGGTGTCTGAGGAA CTTCCAGTTGGACTCAAAACCCCTGGATTCCCCCTCTGCGAGGTCTGGGGTATCTCCCTGCTTAGGTGGC TCTTTGGAGAAAGGCATTTATTTCTCCCAAGGAGGAGGTCACGTGGTCCTAGCCAATTCCGTGTCCTTGG AGCCAGCACTTACGCTCACTCTCAGCATTCGCCCACGAAGTCTCACCGGGGTCTTGATCCACATCGCAAG TCAATCTGGAGAGCACTTAAGTGTCTACATGGAGGCAGGGAAGGTCACGACCTCTATGAACAGTGAGGCA GGTGGGACCGTGACATCAATTACACCGAAGAGATCTCTGTGTGATGGACAATGGCACTCGGTGACAGTCT CCATTAAACAGCACACTCTGCATCTGGAACTGGATACAGACAATAGCTACACAGCTGGGCAGCTTTCCTT CCCACCGAACAGCACCCGAGGGTCACTACACATTGGAGGCGTCCCAGACAAATTGAAAATGCTTACACTC CCTGTGTGGAACTCATTTTTTGGCTGTCTGAAGAATATTCAAGTAAACCACATCCCTGTCCCCATCACAG AAGCCACCGATGTCCAAGGTTCTGTCAGCCTGAATGGCTGCCCTGACCACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_010680
Insert Size	9993 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_010680.1, NP_034810.1
RefSeq Size	10343 bp
RefSeq ORF	9993 bp
Locus ID	16774
UniProt ID	Q61789
Gene Summary	Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (3B). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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