Fbn1 (NM_007993) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI536462; B430209H23; Fib-; Fib-1; Tsk
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225423 representing NM_007993 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCGAGGAGGGCTGCTGGAGGTCGCGCTGGCGTTCGCCCTGCTCCTCGAGTCCTACACGAGCCATG GGGCGGACGCCAATTTGGAGGCTGGGAGCCTGAAGGAGACCAGAGCCAATCGGGCCAAGAGAAGAGGCGG CGGAGGACACGATGCGCTGAAAGGACCCAATGTCTGTGGATCACGTTATAATGCATACTGTTGTCCTGGA TGGAAAACCTTACCTGGTGGAAATCAGTGTATTGTTCCCATTTGCCGGCATTCCTGTGGGGATGGATTCT GCTCGAGGCCAAATATGTGCACTTGCCCGTCTGGTCAGATATCTCCTTCCTGTGGCTCCAGATCCATCCA ACACTGCAGCATCCGCTGTATGAATGGGGGCAGCTGCAGCGATGACCACTGTCTGTGCCAGAAAGGGTAC ATCGGCACTCACTGTGGACAGCCTGTCTGTGAAAGTGGCTGTCTCAACGGAGGGAGGTGTGTGGCCCCAA ATCGGTGTGCTTGCACGTACGGCTTTACTGGACCCCAGTGTGAAAGAGATTACAGAACAGGCCCATGTTT TACTGTGGTAAGCAACCAGATGTGCCAGGGACAGCTCAGCGGGATTGTCTGCACCAAAACACTTTGCTGT GCCACCGTGGGCCGAGCCTGGGGCCACCCCTGTGAGATGTGTCCTGCCCAGCCTCACCCCTGCCGCCGCG GCTTCATTCCCAACATCCGCACTGGAGCTTGTCAAGATGTGGATGAATGTCAGGCCATCCCAGGGATGTG TCAAGGAGGAAATTGCATTAATACCGTTGGATCTTTTGAGTGCAAATGCCCTGCTGGACACAAATTTAAT GAAGTGTCACAAAAATGTGAAGATATTGACGAGTGCAGCACCATTCCTGGAGTCTGCGATGGCGGGGAAT GTACAAACACTGTCAGCAGCTACTTCTGCAAATGTCCCCCTGGTTTTTACACCTCTCCTGATGGCACCAG ATGCGTAGATGTTCGCCCTGGTTACTGCTACACAGCTCTGGCAAACGGGCGCTGCTCTAACCAGCTGCCA CAGTCCATAACCAAAATGCAGTGCTGTTGCGATCTTGGCCGGTGCTGGTCTCCAGGGGTTACTGTTGCTC CCGAGATGTGTCCCATCAGGTCAACTGAGGATTTCAACAAGCTGTGCTCTGTCCCTCTGGTAATTCCCGG GAGACCAGAATATCCTCCCCCACCCATTGGCCCCCTTCCTCCAGTTCAGCCCGTTCCTCCTGGCTATCCT CCTGGGCCTGTGATTCCAGCCCCTCGGCCACCGCCAGAATATCCATATCCATCTCCGTCTCGGGAACCAC CAAGGGTGCTGCCTTTCAACGTTACTGACTACTGTCAACTGGTCCGCTATCTCTGTCAAAATGGGCGCTG CATTCCAACTCCCGGTAGCTACCGCTGCGAGTGCAACAAGGGCTTCCAGCTGGATATCCGTGGCGAATGC ATCGACGTGGATGAGTGTGAGAAGAACCCATGCACTGGTGGCGAGTGCATCAACAACCAGGGCTCCTACA CCTGTCACTGCAGAGCTGGCTACCAGAGCACACTCACCAGAACTGAGTGCAGAGACATAGATGAGTGTCT TCAGAATGGCCGGATCTGCAACAATGGTCGCTGTATCAACACAGACGGCAGCTTCCACTGCGTATGCAAT GCGGGCTTTCATGTCACGCGGGACGGAAAGAACTGTGAAGATATGGATGAGTGCAGCATCCGAAACATGT GCCTAAACGGAATGTGTATTAATGAAGATGGCAGTTTCAAGTGTATTTGCAAACCTGGGTTCCAACTGGC ATCAGATGGCCGCTACTGCAAAGATATCAATGAGTGTGAGACACCTGGGATCTGCATGAACGGACGCTGT GTGAACACGGATGGCTCCTACAGATGCGAATGCTTCCCCGGATTGGCTGTGGGTCTAGACGGACGTGTGT GTGTTGACACACACATGCGGAGCACATGCTATGGAGGATACAGGAGAGGCCAGTGCGTGAAGCCGTTGTT TGGTGCTGTTACCAAATCGGAATGCTGTTGTGCCAGCACTGAGTATGCCTTTGGGGAACCCTGCCAGCCG TGTCCTGCACAGAATTCAGCGGAATATCAGGCACTCTGCAGCAGTGGACCGGGAATGACATCAGCAGGCA CTGATATAAACGAATGTGCATTAGATCCTGATATTTGCCCAAATGGAATTTGTGAAAATCTCCGTGGGAC CTACAAATGTATATGCAACTCGGGATATGAAGTAGACATAACTGGGAAAAACTGTGTCGATATTAATGAG TGTGTGCTGAACAGTCTACTTTGTGACAATGGACAATGTCGAAACACACCTGGAAGTTTTGTCTGCACCT GCCCCAAAGGATTTGTGTACAAACCTGACCTAAAAACCTGTGAAGACATTGATGAATGTGAATCGAGTCC TTGCATTAATGGAGTCTGCAAGAACAGCCCTGGCTCCTTCATTTGTGAATGTTCTCCTGAAAGTACTCTG GACCCAACAAAAACCATCTGCATAGAAACCATCAAGGGCACTTGCTGGCAGACTGTCATCGACGGGCGCT GTGAGATCAACATCAACGGAGCCACCTTGAAGTCCGAGTGCTGCTCCTCCCTTGGTGCTGCGTGGGGGAG CCCGTGCACCATCTGTCAACTTGATCCCATTTGTGGTAAAGGGTTCTCAAGAATTAAAGGCACGCAATGT GAAGATATCAATGAGTGTGAAGTGTTCCCGGGAGTATGCAAGAACGGCCTGTGTGTCAACTCCAGGGGTT CATTCAAGTGCGAGTGTCCCAATGGAATGACTTTGGATGCTACAGGAAGAATCTGTCTTGACATCCGCCT GGAGACCTGCTTCCTCAAGTATGACGATGAAGAGTGCACCTTGCCCATCGCTGGCCGCCACCGAATGGAT GCCTGCTGCTGCTCTGTTGGGGCAGCCTGGGGAACGGAAGAGTGTGAGGAGTGTCCATTGAGAAACAGCC GGGAGTATGAGGAACTCTGTCCCCGAGGACCTGGGTTTGCCACAAAAGACATTACAAATGGGAAACCTTT CTTCAAAGATATCAATGAGTGCAAGATGATACCCAGCCTCTGTACCCACGGCAAGTGCAGGAACACCATT GGCAGCTTCAAGTGTAGGTGTGACAGTGGCTTTGCTCTGGATTCTGAAGAGAGGAACTGTACAGACATTG ATGAGTGCCGCATATCTCCTGACCTCTGTGGCCGAGGCCAGTGTGTGAACACCCCGGGGGACTTTGAATG CAAGTGTGATGAAGGCTATGAAAGTGGCTTCATGATGATGAAGAACTGCATGGATATTGATGAATGTCAG AGAGATCCTCTCCTGTGTCGAGGAGGCATTTGCCACAACACAGAGGGAAGCTATCGCTGCGAATGTCCTC CTGGTCACCAATTGTCCCCAAACATCTCTGCATGCATTGACATCAACGAGTGTGAGCTGAGTGCGAATCT CTGTCCCCATGGGCGTTGTGTGAACCTCATAGGGAAGTACCAGTGTGCCTGCAACCCTGGCTACCACCCC ACTCATGACAGGCTCTTCTGTGTCGATATTGATGAATGCAGCATAATGAACGGTGGTTGTGAGACCTTCT GCACAAACTCTGACGGGAGCTATGAATGTAGCTGTCAGCCAGGCTTCGCGCTAATGCCAGACCAGCGATC GTGCACAGACATTGATGAGTGTGAAGACAACCCCAATATCTGTGATGGTGGCCAGTGCACAAACATACCT GGGGAGTACAGGTGCCTGTGCTATGATGGGTTCATGGCATCTGAAGACATGAAGACTTGTGTGGATGTCA ATGAGTGTGACCTGAATCCAAACATCTGCCTTAGTGGGACCTGTGAAAATACTAAAGGCTCGTTCATCTG CCACTGTGATATGGGATATTCAGGGAAGAAAGGAAAAACGGGCTGTACAGATATCAATGAATGTGAGATC GGAGCACACAACTGTGGCAGACATGCTGTATGCACAAATACAGCCGGGAGCTTCAAGTGCAGCTGCAGTC CCGGCTGGATTGGAGACGGCATTAAGTGCACAGATCTGGATGAATGCTCTAATGGAACCCACATGTGCAG CCAACACGCGGACTGCAAGAACACCATGGGGTCATATCGCTGTCTCTGTAAGGATGGCTATACAGGGGAT GGCTTCACCTGTACAGACCTCGACGAGTGCTCCGAGAACCTGAACCTCTGTGGCAATGGCCAGTGCCTCA ACGCCCCTGGCGGGTACCGCTGTGAATGCGACATGGGCTTCGTGCCCAGTGCTGACGGGAAGGCCTGTGA AGATATCGATGAGTGCTCCCTTCCAAACATCTGTGTCTTTGGAACTTGCCACAACCTCCCGGGCCTCTTC CGTTGCGAGTGTGAGATTGGCTATGAACTGGACCGAAGTGGTGGAAACTGCACAGATGTTAATGAGTGTC TGGATCCCACCACCTGCATCAGTGGAAACTGTGTCAACACTCCCGGTAGTTACACATGCGATTGTCCTCC GGATTTTGAGCTGAATCCAACTCGTGTCGGCTGTGTCGATACTCGCTCTGGAAACTGCTATCTGGATATC CGACCCCGGGGAGACAATGGAGATACAGCCTGCAGCAATGAAATTGGAGTTGGTGTCTCTAAGGCTTCCT GCTGTTGTTCACTGGGTAAAGCTTGGGGAACCCCATGTGAGCTGTGTCCTTCTGTGAACACATCTGAGTA TAAAATTCTTTGCCCTGGAGGAGAAGGTTTTCGTCCAAATCCCATCACCGTTATATTGGAAGACATCGAT GAGTGCCAGGAGCTTCCAGGGCTGTGCCAAGGGGGGAAGTGCATCAATACCTTTGGCAGCTTCCAGTGTC GCTGTCCAACTGGTTACTACCTGAATGAAGACACTCGAGTGTGTGATGATGTGAACGAATGTGAGACTCC TGGAATCTGTGGTCCGGGGACCTGTTACAACACCGTTGGCAACTATACCTGCATTTGTCCTCCAGACTAC ATGCAAGTGAACGGGGGAAATAATTGCATGGACATGAGAAGAAGTCTATGCTACAGAAACTATTACGCTG ACAACCAGACCTGCGATGGAGAACTCCTGTTCAACATGACCAAGAAGATGTGCTGTTGCTCCTACAACAT CGGCAGAGCCTGGAACAAACCCTGTGAACAGTGCCCCATCCCAAGCACAGATGAGTTTGCTACCCTCTGT GGGAGCCAGAGGCCCGGCTTCGTGATTGACATTTATACGGGTTTACCCGTGGATATTGATGAATGCCGGG AGATCCCTGGGGTCTGTGAAAATGGAGTGTGCATCAACATGGTTGGCAGCTTCCGGTGTGAGTGTCCCGT GGGATTCTTCTATAACGACAAGTTACTGGTTTGTGAAGATATCGACGAGTGTCAGAATGGCCCTGTGTGC CAGCGAAATGCGGAATGCATCAACACTGCAGGCAGCTACCGCTGTGACTGTAAGCCCGGCTACCGCCTTA CCTCCACAGGTCAATGCAACGATCGAAACGAGTGCCAAGAAATCCCGAACATATGCAGTCATGGCCAGTG CATCGACACCGTGGGAAGCTTCTACTGCCTTTGTCACACTGGCTTCAAAACAAATGTGGATCAGACCATG TGCTTAGACATAAATGAGTGTGAGAGAGACGCCTGTGGGAACGGGACTTGCAGAAACACGATTGGCTCCT TCAACTGTCGCTGTAACCATGGCTTCATACTGTCTCACAACAATGACTGCATAGATGTTGATGAGTGTGC AACTGGAAACGGGAACCTTTGCAGAAATGGCCAGTGTGTCAATACCGTGGGCTCCTTTCAGTGCAGGTGC AATGAAGGCTATGAGGTGGCTCCGGACGGCAGGACCTGTGTGGATATCAACGAGTGTGTTCTGGATCCTG GGAAATGTGCACCTGGAACCTGTCAGAACCTGGATGGCTCCTACAGATGCATTTGCCCGCCTGGGTATAG TCTACAGAATGACAAGTGTGAAGATATTGATGAGTGTGTTGAAGAGCCAGAAATCTGTGCCTTGGGGACC TGCAGCAACACTGAGGGTAGCTTCAAATGTCTGTGTCCAGAGGGGTTCTCCCTGTCCTCCACTGGAAGAA GGTGCCAAGATTTGCGAATGAGCTACTGCTATGCGAAGTTTGAAGGTGGGAAGTGTTCATCACCCAAATC CAGAAACCATTCCAAGCAGGAGTGCTGCTGTGCTTTGAAGGGAGAAGGCTGGGGAGATCCTTGTGAGTTG TGCCCCACTGAGCCAGATGAGGCTTTCCGCCAGATCTGCCCCTTTGGAAGTGGGATCATTGTGGGCCCTG ATGACTCAGCAGTTGATATGGACGAATGCAAAGAACCTGATGTCTGTAGACATGGGCAGTGCATTAACAC AGACGGCTCCTATCGATGCGAGTGTCCTTTTGGTTATATTCTGGAAGGGAATGAGTGTGTGGATACCGAT GAATGCTCTGTGGGCAATCCTTGTGGAAATGGGACCTGCAAGAATGTGATTGGAGGTTTTGAATGTACCT GTGAGGAGGGGTTCGAGCCTGGCCCAATGATGACTTGTGAAGATATAAATGAATGTGCCCAGAATCCTCT GCTCTGCGCCTTCCGCTGTGTAAATACCTACGGGTCCTATGAATGCAAATGCCCTGTTGGATACGTTCTC CGAGAAGACAGGAGGATGTGTAAAGATGAGGATGAGTGTGCAGAGGGAAAACACGACTGTACTGAGAAGC AAATGGAGTGTAAGAACCTCATTGGTACCTACATGTGCATCTGCGGCCCTGGGTACCAGCGCAGACCCGA TGGAGAGGGCTGCATAGATGAGAATGAGTGTCAGACCAAGCCCGGGATCTGTGAGAATGGGCGTTGCCTC AACACCCTGGGTAGCTACACTTGTGAGTGTAACGATGGCTTCACAGCCAGCCCCACTCAGGATGAGTGCT TGGACAACCGGGAAGGGTACTGCTTTTCGGAGGTCTTGCAAAACATGTGCCAGATTGGCTCAAGCAACAG GAACCCCGTCACCAAGTCCGAGTGCTGCTGTGATGGAGGGAGAGGCTGGGGACCCCACTGTGAGATCTGC CCTTTCGAGGGCACAGTGGCTTACAAGAAGCTCTGTCCCCACGGCCGAGGATTCATGACCAACGGAGCAG ATATTGATGAGTGCAAGGTTATTCATGATGTTTGCCGAAATGGGGAGTGTGTCAACGACAGAGGGTCCTA TCACTGCATCTGTAAAACTGGCTACACTCCGGATATAACAGGGACCGCCTGTGTAGATCTGAATGAATGC AACCAGGCTCCCAAACCCTGCAATTTTATATGCAAAAACACAGAAGGGAGTTACCAGTGTTCCTGCCCGA AGGGCTACATTCTGCAAGAGGATGGAAGGAGCTGCAAAGATCTTGACGAGTGTGCAACCAAGCAGCATAA CTGTCAGTTCCTGTGTGTTAACACCATCGGTGGCTTCACATGCAAATGCCCTCCTGGGTTTACCCAGCAT CACACTGCCTGCATTGATAACAATGAGTGCACGTCTGATATCAACCTGTGTGGGTCCAAGGGTGTTTGCC AGAACACTCCAGGAAGCTTCACCTGTGAATGCCAACGGGGGTTCTCACTCGATCAGAGTGGTGCCAGCTG TGAAGATGTGGACGAGTGTGAGGGTAACCACCGCTGTCAACATGGCTGCCAGAACATCATCGGAGGCTAT AGGTGTAGCTGCCCCCAGGGCTACCTCCAGCACTACCAATGGAACCAGTGTGTAGATGAAAACGAGTGCC TGAGTGCACATGTCTGTGGAGGAGCCTCCTGCCACAACACCCTGGGGAGTTACAAGTGCATGTGTCCCAC CGGCTTCCAGTACGAACAGTTCAGTGGAGGCTGCCAAGACATCAATGAGTGTGGCTCATCCCAGGCCCCC TGCAGTTACGGTTGCTCTAATACTGAGGGTGGCTACCTGTGTGGCTGTCCACCAGGATACTTCCGGATAG GCCAAGGGCATTGTGTTTCTGGAATGGGCATGGGCCGAGGCGGCCCAGAGCCACCTGCCAGCAGCGAGAT GGACGACAACTCACTGTCCCCAGAGGCCTGCTATGAGTGTAAGATCAACGGCTACCCAAAACGAGGCCGG AAACGGAGAAGCACGAACGAAACGGATGCCTCCGACATCCAGGACGGGTCTGAGATGGAAGCCAACGTGA GCCTCGCCAGCTGGGATGTGGAGAAGCCGGCTAGCTTTGCTTTCAATATTTCCCATGTCAATAACAAGGT CCGAATCCTAGAGCTCCTGCCGGCCCTCACAACTCTGATGAACCACAACAGATACTTGATTGAATCTGGA AATGAAGATGGCTTCTTTAAAATCAACCAGAAAGAAGGGGTCAGCTACCTCCACTTCACGAAGAAGAAGC CGGTGGCTGGGACCTACTCCTTACAAATCAGCAGCACCCCACTTTATAAAAAGAAAGAACTTAACCAGTT AGAAGACAGATATGACAAAGACTACCTCAGTGGTGAACTGGGCGATAACCTGAAGATGAAAATTCAGATC TTGCTGCATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_007993
Insert Size	8622 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_007993.2, NP_032019.2
RefSeq Size	9900 bp
RefSeq ORF	8622 bp
Locus ID	14118
UniProt ID	Q61554
Gene Summary	This gene encodes a member of the fibrillin family of proteins. The encoded preproprotein is proteolytically processed to generate two proteins including the extracellular matrix component fibrillin-1 and the protein hormone asprosin. Fibrillin-1 is an extracellular matrix glycoprotein that serves as a structural component of calcium-binding microfibrils. Asprosin, secreted by white adipose tissue, has been shown to regulate glucose homeostasis. Homozygous knockout mice for this gene exhibit impaired aortic development and early postnatal death, which was attributed to a deficiency in the fibrillin-1 protein. Mice with a hypomorphic allele of this gene exhibit impaired glucose homeostasis, likely due to a reduction in serum asprosin levels. [provided by RefSeq, Apr 2016]

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