Ubr5 (NM_001081359) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Edd; Edd1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225414 representing NM_001081359 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGTCCATCCATTTCGTGGTCCACCCGCTGCCGGGCACCGAGGACCAGCTCAATGACAGGTTACGGG AAGTTTCAGAGAAGCTGAACAAGTACAATTTGAACAGCCACCCACCTCTGAATGTACTGGAGCAGGCTAC TATTAAACAGTGTGTGGTGGGACCCAATCACGCCGCGTTTCTTCTTGAGGATGGCAGAATTTGTAGGATT GGTTTTTCAGTACAACCAGACAGACTGGAATTGGGTAAACCTGATAATAATGATGGGTCAAAACTGAACA GCAGCTCGGGGACAGGGAGGACCTCTAGGCCAGGAAGGACAAGTGACTCCCCGTGGTTTCTCTCAGGTTC TGAGACTCTGGGCAGGTTGGCAGGCAACACTCTGGGAAGCCGCTGGAGTTCTGGCGTGGGGGGAAGTGGA GGAGGCTCCTCTGGTCGGTCGTCTGCTGGAGCTCGTGATTCCCGGCGACAGACTCGAGTCATTCGGACTG GCCGGGATCGGGGGTCTGGACTCCTGGGCAGTCAGCCCCAGCCAGTTATTCCAGCATCTGTCATTCCTGA GGAGCTGATATCACAGGCACAAGTTGTTCTACAAGGCAAATCGAGAAGTGTCATTATTCGAGAACTTCAG AGAACAAATCTTGATGTCAACCTTGCTGTAAATAACTTACTGAGCCGGGATGATGAGGATGGAGATGACG GTGATGACACAGCCAGTGAATCTTACCTGCCGGGAGAAGATCTTATGTCTCTTCTTGACGCTGACATTCA CTCTGCCCACCCAAGTGTCATTATTGATGCTGATGCCATGTTTTCTGAGGACATTAGCTATTTTGGTTAT CCTTCTTTTCGTCGTTCATCACTCTCTAGGCTAGGCTCATCACGAGTTCTCCTTCTTCCCTTAGAGAGAG ACTCAGAGCTTCTGCGGGAACGGGAGTCTGTTCTACGTTTACGGGAGCGGCGGTGGCTTGATGGAGCCTC ATTTGATAATGAAAGGGGCTCTACCAGCAAAGAGGGAGAGTCAAACCCGGATAAGAAGAACACGCCTGTT CAGAGTCCAGTGTCTCTGGGAGAAGACTTACAGTGGTGGCCTGATAAGGATGGAACAAAATTTACCTGCA TTGGGGCTCTGTATTCTGAGCTTCTTGCTGTCAGCAGCAAAGGAGAACTTTATCAATGGAAATGGAGTGA ATCTGAGCCTTACAGAAATGCCCAGAATCCTTCATTGCATCATCCACGAGCAACATTTTTGGGGTTGACC AATGAAAAGATAGTCCTCTTGTCTGCAAATAGCATAAGAGCAACTGTAGCTACTGAAAATAACAAGGTTG CTACATGGGTGGATGAAACATTAAGTTCTGTGGCTTCTAAGTTAGAACACACAGCACAGACTTACTCTGA ACTTCAAGGAGAACGGATAGTTTCTTTACATTGCTGTGCCCTTTATACCTGTGCCCAGCTGGAGAATAAT TTGTATTGGTGGGGTGTTGTTCCTTTCAGTCAAAGGAAGAAAATGTTAGAGAAAGCTAGAGCAAAAAATA AAAAGCCCAAATCCAGTGCTGGTATTTCTTCCATGCCAAACATCACTGTTGGAACTCAGGTGTGCTTGAG AAATAACCCTCTCTACCATGCTGGAGCGGTTGCCTTTTCAATTAGTGCTGGCATTCCTAAAGTTGGTGTC TTGATGGAGTCAGTATGGAATATGAATGATAGCTGTAGATTTCAACTTCGGTCTCCAGAGAGCCTGAAGA GCATGGAAAAAGCCAGCAAAACTCTTGAGACAAAGCCCGAGAGTAAGCAGGAACCAGTAAAAACAGAAAT GGGCCCTCCACCATCACCAGCATCCACCTGCAGCGACGCGTCCTCAATTGCCAGCAGTGCATCCATGCCA TACAAACGACGTCGGTCAACTCCTGCCCCGAGAGAAGAGGAGAAGGTGAACGAGGAGCAGTGGCCCCTTC GGGAAGTTGTTTTTGTTGAAGATGTTAAGAACGTTCCTGTTGGCAAGGTGCTGAAGGTAGATGGTGCCTA TGTCGCTGTAAAGTTTCCAGGAACATCTACTAACACCACCTGTCAGAACAGCTCTGGTCCAGACGCCGAC CCTTCCTCGCTCCTGCAGGACTGCAGGCTGCTTCGAATTGATGAGCTGCAGGTTGTCAAAACGGGCGGCA CCCCAAAAGTTCCCGACTGTTTTCAGAGGACTCCCAAAAAGCTTTGTATACCCGAGAAGACTGAAATACT AGCAGTAAATGTGGATTCCAAAGGTGTCCATGCTGTTCTGAAGACTGGAAGCTGGGTTCGGTACTGTGTC TTTGATCTTGCCACAGGGAAGGCTGAACAAGAGAATAATTTCCCAACAAGCAGTGTTGCTTTTCTTGGTC AGGATGAGAGGAGTGTCGCTATCTTCACTGCTGGGCAGGAATCTCCTATTGTTCTTCGAGATGGAAATGG TACTATCTACCCAATGGCCAAGGATTGCATGGGAGGAATACGGGATCCTGACTGGCTTGATCTCCCACCA ATCAGTAGTCTTGGGATGGGTGTACATTCTTTAATAAATCTTCCTGCCAACTCAACAATCAAAAAGAAAG CAGCTATTATCATCATGGCTGTAGAGAAGCAAACCTTAATGCAGCACATTCTCAGATGTGACTACGAGGC CTGCCGACAGTATCTCGTGAATCTGGAGCAAGCCGTGGTTTTAGAGCAGAACCTGCAGATGTTACAGACG TTCATCAGCCACAGATGTGATGGGAACCGGAACATTTTACATGCTTGTGTATCAGTTTGCTTTCCAACCA GCAATAAGGAAACTAAAGAAGAAGAGGAAGCTGAGCGCTCAGAAAGAAATACATTTGCTGAAAGGCTTTC TGCTGTGGAGGCCATTGCAAATGCAATATCAGTTGTTTCAAGTAACGGCCCAGGTAATCGGGCTGGATCA TCAAATAGTCGAAGTTTGAGATTACGAGAAATGATGAGACGTTCATTGAGAGCAGCTGGTTTGGGTAGAC ATGAGGCTGGAGCTTCATCCAGTGACCACCAGGATCCAGTTTCACCCCCCATAGCTCCCCCTAGCTGGGT TCCTGACCCTCCTTCAATGGATCCTGATGGTGATATTGATTTTATCCTGGCCCCCGCTGTGGGATCTCTT ACCACAGCAGCGACTGGCAGTGGTCAAGGACCCAGCACCTCCACCATTCCAGGTCCTTCCACAGAGCCAT CTGTAGTAGAATCCAAGGATCGAAAGGCAAACGCACATTTCATATTAAAATTACTATGCGACAGTGCGGT TCTGCAGCCTTATCTACGAGAGCTTCTTTCTGCCAAGGATGCAAGAGGAATGACCCCGTTTATGTCAGCT GTAAGTGGCCGGGCTTATCCTGCTGCAATCACCATCCTAGAAACAGCCCAGAAGATTGCCAAAGCTGAAG TATCTGCAAGTGAAAAGGAGGAAGATGTGTTCATGGGAATGGTTTGCCCGTCAGGTACCAACCCTGACGA CTCCCCTTTGTATGTCTTATGTTGCAACGACACCTGCAGCTTTACCTGGACTGGAGCAGAGCACATTAAC CAGGACATTTTTGAATGTCGGACTTGTGGTTTACTGGAGTCGCTTTGTTGTTGTACAGAATGTGCAAGGG TTTGTCATAAAGGGCACGATTGCAAACTCAAGAGGACTTCACCAACAGCTTATTGTGATTGTTGGGAGAA ATGTAAATGTAAAACATTGATTGCTGGACAGAAGTCTGCTCGTCTTGATCTACTTTATCGCCTGCTTACT GCTACAAATCTGGTCACCCTACCAAACAGTAGGGGAGAGCACCTTTTGCTGTTCTTAGTACAGACCGTGG CCAGGCAGACAGTGGAGCACTGTCAGTATAGGCCTCCACGAATCAGGGAGGATCGCAACAGGAAAACAGC CAGTCCTGAAGATTCAGATATGCCTGACCATGACTTAGAGCCCCCAAGGTTTGCCCAGCTTGCCCTAGAG CGCGTCCTTCAGGACTGGAATGCTTTGAGATCTATGATTATGTTTGGGTCACAAGAGAACAAAGATCCTC TGAGTGCCAGCAGCAGGATAGGCCATCTTTTGCCAGAAGAGCAGGTCTACCTCAACCAGCAGAGCGGCAC GATTCGGCTCGACTGTTTCACTCATTGCCTTATTGTCAAGTGTACAGCAGATATTTTGCTTTTAGATACT CTGCTAGGCACCTTAGTAAAAGAACTCCAAAACAAGTACACACCCGGACGCCGGGAAGAAGCAATTGCCG TGACAATGAGGTTTCTACGATCTGTGGCAAGAGTGTTTGTCATTCTTAGTGTGGAAATGGCTTCGTCCAA AAAGAAAAACAACTTTATTCCACAGCCAATTGGAAAATGCAAACGTGTGTTTCAAGCACTGCTTCCTTAT GCTGTAGAAGAATTGTGTAATGTGGCAGAGTCTCTGATTGTTCCTGTCAGAATGGGGATTGCCCGGCCGA CAGCACCGTTTACTCTGGCCAGTACTAGCATAGATGCCATGCAGGGCAGTGAAGAATTATTTTCAGTAGA ACCATTACCACCACGGCCGTCATCAGATCAGGCAAGCAGCTCCAGTCAGTCTCAGTCATCTTACATCATT CGGAACCCACAGCAGAGGCGCATCAGCCAGTCACAGCCCGTCAGAGGCCGAGACGAGGAGCAGGATGATA TTGTGTCAGCAGACGTGGAAGAGGTTGAGGTGGTGGAAGGCGTGGCTGGAGAGGAGGATCATCATGATGA ACAGGAAGAGCACGGGGAAGAGAATGCTGAGGCTGAGGGACATCACGATGAGCATGATGAAGACGGAAGT GACATGGAGCTAGATTTGTTAGCAGCAGCTGAAACGGAAAGTGACAGTGAAAGTAACCATAGTAACCAAG ATAATGCTAGTGGGCGCAGAAGTGTTGTCACCGCAGCCACTGCTGGCTCAGAAGCAGGAGCGAGCAGTGT TCCTGCCTTTTTTTCTGAAGATGACTCTCAGTCAAATGACTCAAGTGACTCTGACAGCAGTAGCAGTCAG AGTGATGACATAGAGCAGGAGACCTTTATGCTCGATGAGCCACTAGAAAGGACAACGAATAGCTCCCATG CAAATGGTGCTGCCCAGGCTCCTCGCTCCATGCAGTGGGCTGTCCGAAATCCGCAGCATCAGCGAGCAGC AAGCACAGCTCCGTCCAGCACATCTACGCCAGCAGCAAGTTCTGCAGGTTTGATTTATATTGATCCTTCA AATTTACGCCGGAGCGGCACCATCAGTACAAGTGCTGCAGCGGCAGCAGCTGCTTTGGAAGCTAGCAATG CCAGCAGCTACTTAACATCTGCAAGCAGTTTAGCCAGGGCTTACAGCATTGTCATCAGACAAATCTCGGA CTTGATGGGCCTTATCCCTAAGTATAATCACCTAGTATACTCTCAGATTCCAGCAGCTGTGAAATTGACT TACCAAGATGCAGTCAACTTACAGAACTATGTAGAAGAAAAGCTTATTCCTACGTGGAACTGGATGGTCA GTGTTATGGATTCTACTGAAGCTCAATTACGTTATGGTTCTGCACTAGCATCTGCTGGTGACCCAGGACA CCCAAATCATCCCCTTCATGCCTCTCAGAATTCAGCTCGAAGAGAGAGGATGACTGCACGGGAAGAGGCC AGTCTAAGAACGCTTGAAGGCAGACGACGTGCTACATTGCTGAGCGCCCGCCAGGGAATGATGTCTGCTC GAGGAGACTTTCTCAATTATGCTCTGTCTTTAATGAGATCTCATAATGATGAGCATTCTGATGTTCTTCC AGTATTGGATGTGTGCTCATTGAAACATGTGGCATATGTTTTCCAAGCCCTTATATACTGGATTAAAGCA ATGAATCAGCAGACAACATTAGACACTCCTCAACTGGAACGCAAAAGGACACGAGAACTTCTGGAACTGG GTATTGATAATGAAGATTCAGAACATGAAAATGATGATGACACCAGTCAAAGTGCTACTTTGAATGATAA GGATGACGACTCTCTTCCTGCAGAAACTGGCCAAAACCATCCATTTTTCCGACGTTCAGACTCTATGACA TTCCTTGGATGTATACCTCCAAATCCATTTGAAGTGCCTTTGGCTGAAGCCATACCCTTGGCTGATCAGC CACATTTGTTGCAGCCAAATGCTAGAAAGGAAGATCTTTTTGGTCGCCCAAGTCAGGGTCTTTATTCCTC ATCTGCCGGAAGTGGGAAATGCATAGTAGAGGTTACAATGGACAGAAATTGTCTCGAGGTTCTTCCAACT AAAATGTCTTATGCTGCCAATCTAAAAAATGTAATGAACATGCAAAACCGGCAGAAGAAAGAAGGGGAAG AACAGAGCCTGCTGGCCGAAGAAGCTGACAGTTCAAAGCCAGGTCCATCCGCTCCTGATGTTGCTGCCCA GTTGAAAAGCAGTTTACTAGCAGAGATAGGACTCACGGAAAGTGAAGGGCCCCCCCTTACATCTTTCAGG CCTCAGTGCAGTTTCATGGGCATGGTTATCTCCCATGACATGCTGCTGGGACGCTGGCGCCTGTCTTTAG AACTGTTCGGCAGAGTATTCATGGAAGATGTTGGTGCAGAGCCTGGATCGATTTTAACTGAATTGGGTGG TTTTGAGGTAAAAGAATCAAAGTTCCGTAGAGAGATGGAAAAACTGAGAAACCAGCAATCAAGAGATTTA TCACTAGAGGTCGATCGCGATCGGGATCTTCTCATTCAGCAGACTATGAGACAGCTTAACAACCATTTTG GCCGAAGATGTGCTACCACGCCAATGGCTGTGCACAGAGTCAAAGTCACCTTTAAGGATGAACCAGGAGA GGGCAGTGGTGTAGCAAGAAGTTTCTATACAGCCATTGCACAAGCGTTTTTATCAAATGAAAAATTGCCA AATCTAGATTGTATCCAAAATGCCAACAAAGGTACTCATACAAGTTTAATGCAAAGATTGAGGAACAGAG GTGAAAGAGACCGGGAGAGAGAGAGAGAACGGGAAATGAGGAGGAGCAGTGGCTTGCGTGCAGGATCTCG AAGAGACAGGGATAGAGACTTCAGGAGACAGCTCTCCATTGACACCAGGCCTTTCAGACCTGCTTCTGAG GGGAATCCTAGTGATGACCCTGACCCTCTTCCAGCTCATCGGCAGGCACTTGGAGAGAGGCTTTACCCCC GAGTGCAAGCAATGCAGCCCGCATTTGCAAGTAAAATCACTGGCATGCTGTTGGAGTTATCCCCAGCTCA GCTGCTTCTCCTTCTAGCAAGTGAAGATTCTCTGAGGGCGAGAGTCGACGAGGCCATGGAACTCATTATT GCTCATGGACGGGAAAATGGAGCTGACAGTATTTTGGACCTCGGATTATTAGACTCTTCAGAAAAGGTAC AGGAAAACAGAAAGCGCCATGGCTCTAGTAGAAGTGTAGTGGATATGGATTTAGAGGACACAGATGATGG TGATGACAACGCTCCCTTGTTTTACCAACCTGGGAAGAGAGGATTTTATACTCCGAGACCTGGCAAAAAC ACAGAAGCAAGGCTGAATTGTTTCAGAAACATCGGCAGGATTCTTGGACTATGTCTGTTGCAAAATGAAC TATGTCCTATCACATTGAATAGGCATGTAATTAAAGTGTTGCTTGGTAGAAAAGTCAACTGGCATGACTT TGCTTTTTTCGACCCTGTAATGTATGAGAGTTTACGACAACTGATCCTTGCATCTCAGAGTTCAGATGCT GATGCTGTTTTCTCAGCAATGGATTTGGCATTTGCAATTGACCTGTGTAAAGAAGAGGGTGGAGGACAGG TTGAACTTATTCCAAATGGTGTAAATATACCAGTCACTCCGCAGAATGTATATGAGTATGTAAGGAAATA TGCAGAGCATAGAATGTTGGTGGTTGCAGAACAGCCATTGCACGCAATGAGAAAGGGTCTACTAGACGTG CTTCCAAAAAATTCCTTAGAAGACTTAACAGCTGAGGACTTCCGGCTCTTGGTGAACGGCTGTGGTGAAG TCAATGTGCAGATGCTGATCAGTTTCACTTCTTTCAATGATGAGTCAGGAGAAAATGCTGAGAAGCTCCT CCAGTTCAAACGGTGGTTCTGGTCAATAGTAGAGAAAATGAGCATGACAGAACGGCAGGACCTGGTTTAC TTCTGGACATCTAGCCCATCATTGCCAGCCAGTGAAGAAGGTTTTCAGCCTATGCCCTCGATCACAATAA GACCACCAGATGACCAACATCTTCCTACTGCAAATACTTGTATTTCTCGACTTTATGTCCCACTCTATTC CTCTAAACAGATTCTCAAACAAAAATTACTACTCGCCATTAAGACCAAGAATTTTGGTTTTGTGTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001081359
Insert Size	8397 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001081359.3, NP_001074828.2
RefSeq Size	9791 bp
RefSeq ORF	8397 bp
Locus ID	70790
UniProt ID	Q80TP3
Gene Summary	E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Ubiquitinates acetylated PCK1. Also acts as a regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes (By similarity). Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Involved in maturation and/or transcriptional regulation of mRNA by activating CDK9 by polyubiquitination. May play a role in control of cell cycle progression. May have tumor suppressor function. Plays an essential role in extraembryonic development. Regulates DNA topoisomerase II binding protein (TopBP1) for the DNA damage response.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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