Piezo2 (NM_001039485) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5930434P17; 9030411M15Rik; 9430028L06Rik; Fam38b; Fam38b2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225402 representing NM_001039485 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCGGAAGTGGTGTGCGGGCTGATCTTCAGGCTGCTGCTTCCCATCTGCCTGGCAGTAGCATGTG CGTTCCGGTACAATGGGCTCTCCTTTGTCTACCTTATCTACCTCTTGCTCATTCCTCTGTTCTCAGAACC AACCAAAGCAACGATGCAAGGACACACAGGGCGGTTGCTACAGTCCCTGTGCATCACCAGCCTCTCATTT CTGCTGCTTCACATCATTTTCCACATCACACTGGCCAGTCTGGAAGCTCAACACCGCATCACACCTGCTT ACAACTGCTCAACATGGGAAAAGACCTTCCGGCAGATTGGCTTTGAAAGCTTGAAGGGAGCTGATGCCGG CAATGGCATCAGGGTATTTGTGCCTGATATCGGGATGTTCATTGCAAGTCTGACCATCTGGCTGGTCTGC AGAACCATTGTTAAGAAACCAGACACAGAAGAAATAGCCCAATTGAATTCTGAGTGTGAAAATGAAGAAT TGGCTGGAGGAGAAAAGATGGATTCAGAGGAGGCGCTGATTTATGAAGAGGACTTAGATGGAGAAGAAGG CATGGAAGGAGAGCTAGAAGAAAGCACAAAACTAAAAATACTTCGCAGGTTCGCCTCTGTCGCCTCCAAA CTGAAGGAGTTCATTGGCAATATGATCACCACGGCGGGCAAGGTCGTGGTGACCATCCTACTGGGCTCCT CGGGCATGATGCTGCCATCTTTGACCTCGGCTGTGTACTTCTTTGTATTTCTGGGCCTGTGTACCTGGTG GTCCTGGTGCCGAACTTTCGACCCATTGCTGTTCGGCTGCCTCTGTGTCCTGCTGGCAATCTTCACTGCT GGGCACCTGATTGGACTTTATCTGTACCAGTTCCAATTCTTCCAAGAAGCGGTCCCGCCCAATGACTACT ATGCAAGGTTGTTTGGGATCAAGTCCGTTATCCAGACAGACTGTGCCAGCACCTGGAAGATCATAGTAAA CCCAGACCTGTCCTGGTATCACCATGCCAACCCCATCCTCCTGCTGGTGATGTACTATACGCTGGCCACG CTGATTCGCATCTGGCTACAAGAGCCTCTTGTGCAAGAGGAGATGGCGAAGGAAGATGAGGGTGCCCTGG ATTGCAGCTCCAATCAGAACACGGCAGAGAGGAGGCGGAGCCTGTGGTATGCAACCCAGTACCCAACAGA TGAGAGAAAACTTTTATCCATGACCCAAGATGACTACAAACCATCTGATGGCCTGCTGGTGACCGTGAAT GGCAACCCTGTGGACTACCACACCATCCACCCAAGCCTGCCCATAGAGAATGGCCCTGCCAAGACGGACC TGTACACCACTCCCCAGTACCGGTGGGAGCCTTCGGAGGAGTCCTCAGAAAAGAAAGAGGAAGAAGAGGA CAAGAGAGAAGACTCCGAAGGGGAAGGGAGCCAGGAGGAGAAAAGGAGTGTGAGAATGCACGCCATGGTC GCCGTGTTCCAGTTCATCATGAAGCAGAGTTACATCTGTGCCCTCATCGCCATGATGGCATGGAGCATCA CCTACCACAGCTGGCTGACTTTCGTGCTGTTGATCTGGTCCTGCACCCTCTGGATGATTCGCAACAGGAG GAAGTATGCCATGATCAGTTCTCCTTTCATGGTCGTCTATGCGAATCTGCTGCTGGTGCTGCAGTACATA TGGAGCTTCGAGCTCCCCGAGATTAAGAAGGTCCCCGGGTTTCTAGAAAAGAAAGAGCCGGGAGAACTTG CTTCAAAGATTCTCTTCACCATCACGTTCTGGCTGCTCCTGAGGCAGCATCTCACAGAGCAGAAGGCCCT GAGGGAAAAGGAAGCACTTCTTTCTGAAGTCAAAATTGGAAGTCAAGAACTTGAAGAAAAGGAAGATGAG GAGCTCCAAGATGTACAAGTGGAAGGGGAGCCCACAGAGAAGGAAGAAGAAGAGGAAGAGGAAATAAAGG AAGAAAGGCATGAGGTAAAGAAGGAAGAGGAAGAGGAAGTGGAGGAAGATGATGACCAGGACATCATGAA GGTGCTGGGTAATCTGGTGGTGGCCCTCTTCATCAAGTATTGGATCTACGTCTGCGGGGGCATGTTCTTT TTTGTCAGCTTTGAGGGGAAAATCGTCATGTACAAAATCATCTACATGGTGCTGTTCCTGTTCTGCGTGG CCTTGTACCAGGTGCACTACGAGTGGTGGAGGAAGATCCTGAAGTACTTCTGGATGTCGGTGGTCATTTA TACCATGTTGGTGCTCATCTTCATATACACTTACCAGTTTGAAAACTTCCCAGGCCTGTGGCAAAACATG ACCGGATTAAAGAAAGAAAAGCTCGAGGACCTCGGCCTGAAGCAGTTCACGGTCGCTGAACTGTTCACTC GCATATTCATCCCCACCTCCTTTCTACTCGTCTGCATCCTACACCTCCACTACTTCCACGATCGGTTCCT GGAACTTACAGACCTCAAGTCCATCCCCAGCAAAGAAGACAACACCATCTACAGCCATGCCAAAGTCAAT GGTCGCGTGTACCTAATAATAAATAGGTTGGCCCACCCTGAAGGAAGTCTTCCAGACTTAGCCATCATGA ATATGACGGCCAGCCTTGATAAGCCTGAGGTGCAGAAGTTGGCAGAGTCTGGGGAGGAGAGGCCAGAAGA GTGTGTCAAGAAGACGGAGAAGGGTGAGGCTGGGAAAGACAGTGATGAGTCTGAGGAGGAGGAAGATGAA GAAGAGGAATCCGAGGAAGAAGAAAGCTCAGACCTCAGGAACAAGTGGCACCTGGTGATTGACCGGCTCA CTGTGCTCTTTCTAAAATTCCTGGAGTATTTCCACAAGTTGCAGGTGTTTATGTGGTGGATCTTGGAGCT GCACATCATCAAAATCGTCTCATCGTACATTATCTGGGTGACTGTGAAAGAGGTGTCTCTGTTCAACTAT GTATTTTTGATTTCTTGGGCATTTGCTCTGCCATATGCCAAACTCCGTCGTGCGGCTTCCAGTGTCTGCA CTGTCTGGACATGTGTGATCATCGTCTGCAAAATGTTGTACCAGCTGCAAACCATTAAACCTGAGAACTT TTCTGTCAACTGCTCCTTGCCCAATGAAAATCAAACCAACATTCCCCTTCATGAGTTGAACAAGTCTCTT CTCTACAGTGCCCCTGTTGATCCCACTGAGTGGGTAGGCCTGAGGAAGTCCTCTCCTTTGCTCGTCTACC TGAGGAACAACCTCCTGATGCTGGCGATTTTGGCCTTTGAAGTCACCGTTTACCGCCATCAGGAGTACTA TCGAGGCCGAAACAACCTGACTGCTCCAGTGTCTAAAACTATCTTTCATGACATCACAAGGTTACATCTA GATGATGGGCTTATCAATTGTGCCAAATATTTTGTAAATTACTTCTTCTACAAGTTTGGCCTAGAGACCT GTTTCCTAATGTCCGTGAATGTAATTGGACAGCGAATGGACTTCTACGCCATGATTCATGCCTGTTGGTT GATCGGTGTCTTGTATCGACGCAGAAGAAAAGCCATTGCCGAGGTCTGGCCCAAGTACTGCTGCTTCCTG GCTTGCATCATCACCTTCCAGTACTTCGTCTGCATCGGCATCCCCCCTGCCCCTTGCCGAGATTATCCAT GGAGATTTAAGGGAGCCTACTTCAATGATAACATTATCAAGTGGCTGTACTTCCCGGATTTCATTGTGCG GCCCAACCCCGTGTTTCTTGTCTATGACTTCATGCTGCTCCTGTGTGCTTCCTTACAACGACAGATCTTT GAGGACGAAAACAAGGCAGCCGTGCGCATCATGGCCGGGGACAATGTCGAGATCTGCATGAACCTTGATG CAGCCTCGTTCAGCCAGCATAACCCTGTGCCAGATTTCATTCACTGCAGATCTTACTTGGACATGTCCAA AGTGATCATCTTCAGCTACCTCTTCTGGTTCGTCCTCACCATCATCTTCATTACGGGAACAACCAGGATC AGCATATTTTGCATGGGCTACTTGGTGGCCTGTTTCTATTTCCTGCTCTTTGGAGGGGATCTGCTGCTGA AGCCCATCAAGAGCATCCTTCGTTACTGGGACTGGCTGATTGCATACAACGTGTTTGTGATCACCATGAA GAATATACTGTCAATAGGAGCCTGTGGATACATTGGCGCACTAGTAAGAAACAGCTGCTGGTTGATCCAA GCGTTCAGCCTGGCCTGCACCGTGAAAGGCTATCAGATGCCTGAAGATGACTCCAGATGCAAGCTGCCCA GTGGGGAGGCAGGGATCATCTGGGACAGCATCTGCTTTGCGTTTCTGCTGCTGCAGAGGAGAGTCTTCAT GAGCTACTACTTCCTGCATGTCGTGGCTGACATTAAAGCATCCCAGATCCTGGCATCCAGAGGGGCCGAA CTTTTCCAGGCTACAATTGTGAAAGCTGTAAAGGCAAGAATTGAAGAAGAGAAAAAATCCATGGACCAGC TGAAGAGACAGATGGATCGCATCAAAGCCCGGCAACAAAAGTACAAAAAGGGTAAGGAGAGGATGCTGAG CTTGACCCAGGAGTCAGGGGAAGGCCAGGACATCCAGAAAGTCTCAGAAGAAGATGATGAAAGAGAAGCA GACAAACAGAAAGCCAAGGGCAAAAAAAAGCAGTGGTGGCGGCCTTGGGTTGATCATGCTTCCATGGTCA GGAGTGGAGATTATTATTTGTTTGAAACGGATAGTGAAGAGGAGGAAGAAGAGGAACTTAAAAAGGAGGA TGAAGAACCCCCACGGAAGTCAGCATTCCAGTTCGTCTACCAAGCCTGGATTACTGATCCTAAAACGGCA CTCCGACAGAGGAGGAAAGAGAAGAAAAAGTTGGCCAGAGAAGAGCAGAAGGAGAGACGCAAAGGATCTG GGGACGGTCCAGTGGAATGGGAAGATCGAGAGGATGAGCCAGTCAAAAAGAAATCTGATGGACCAGATAA TATCATCAAGAGGATATTTAATATCCTGAAGTTCACATGGGTTCTGTTCCTGGCCACAGTGGATAGTTTC ACCACTTGGCTCAACTCCATCTCAAGGGAACACATCGACATATCCACAGTCCTGCGGATTGAAAGATGCA TGCTGACTCGAGAGATAAAAAAGGGCAATGTTCCAACACGGGAAAGCATCCACATGTACTACCAGAACCA CATTATGAACCTTTCCCGAGAATCCGGCCTGGACACAATCGACGAGCACTCGGGGGCTGGCTCCAGGGCC CAGGCAGCACATAGGATGGATAGCTTAGATTCCCGGGACAGTATCTCCAGCTGCTACACTGAAGCGACCC TGCTGATCTCAAGGCAGTCCACCCTTGATGATTTAGATGGACAAGACCCCGTTCCTAAAACAAGCGAGCG TGCTCGGCCTAGGCTCCGTAAAATGTTCAGCCTGGACATGTCCTCTTCGTCAGCTGACAGTGGCAGTGTA GCATCAAGCGAGCCCACACAGTGTACCATGCTGTATTCGCGCCAAGGAACTACGGAAACCATCGAGGAGG TGGAGGCTGAAGCTGAGGAGGAGGTGGTAGAAGGTCTGGAGCCTGAGCTCCATGATGCAGAAGAAAAGGA ATACGCAGCTGAATATGAAGCAGGGGTAGAGGAGATCAGCCTCACTCCAGATGAGGAATTGCCTCAGTTC TCTACAGATGACTGTGATGTGGAGGCGCCGCCCTCTTACAGCAAGGCTGTCAGCTTTGAGCATCTATCGT TTGCGTCGCAAGATGACTCTGGGGCCAAGAACCACATGGTGGTCAGTCCTGATGACAGCCGTACAGACAA GCTGGAGTCAAGCATCTTACCACCTCTGACTCATGAGCTGACAGCCAGCGATCTGCTGATGAGCAAGATG TTCCATGATGATGAGCTTGAGGAGTCTGAGAAGTTCTACGTGGACCAGCCCCGGTTTTTGCTGCTATTCT ATGCCATGTATAACACCCTGGTGGCCCGCTCAGAAATGGTGTGCTATTTCGTGATTATCCTGAACCACAT GACCTCTGCCTCCATCATCACTCTCCTGTTGCCCATCTTGATCTTCCTCTGGGCCATGCTGTCTGTTCCC AGGCCCAGCCGACGCTTCTGGATGATGGCCATCGTCTACACTGAGGTGGCGATCGTAGTGAAATACTTCT TCCAGTTCGGCTTCTTTCCCTGGAATAAGGACTTGGAAATATACAAGGAAAGGCCCTACTTTCCACCGAA CATCATCGGGGTGGAGAAGAAGGAGGGCTATGTTCTTTATGACCTCATTCAGCTGCTGGCTCTATTCTTC CATCGCTCCATTCTGAAGTGTCATGGTCTATGGGACGAGGATGACATTGTTGACAGTAACACAGACAAGG AAGGATCGGATGACGAGCTGTCCCTCGATCAGGGCAGAAGAGGCTCCTCAGACTCACTGAAGTCTATCAA CCTGGCTGCCTCTGTAGAGTCTGTCCACGTGACCTTCCCCGAGCAGCCAGCGGCCATCAGGAGAAAACGC TCCTGCAGTAGCTCTCAGATCTCCCCACGGTCCAGCTTCTCTTCAAATAGGTCCAAAAGAGGCAGCACAA GCACCCGGAACAGTAGCCAGAAAGGAAGCAGTGTTTTAAGCCTTAAACAGAAAAGCAAACGAGAACTTTA CATGGAAAAGCTTCAAGAGCATTTAATCAAAGCAAAGGCGTTTACCATAAAGAAGACTCTACAGATCTAT GTCCCCATTCGGCAATTCTTCTATGACCTCATCCACCCAGACTACAGTGCTGTGACTGATGTATACGTGC TGATGTTTCTGGCTGACACTGTAGACTTCATTATCATCGTCTTTGGATTCTGGGCCTTTGGGAAACACTC GGCAGCTGCAGATATCACCTCTTCTCTATCAGAAGACCAGGTCCCCGGGCCATTCCTGGTGATGGTCCTC ATTCAGTTTGGAACCATGGTGGTGGACCGTGCACTCTACCTCAGGAAGACTGTGCTGGGCAAGGTCATCT TCCAGGTCATCCTCGTGTTTGGGATTCACTTCTGGATGTTCTTCATCTTACCCGGTGTGACTGAGAGGAA ATTCAGCCAGAACCTGGTGGCACAGCTTTGGTACTTTGTGAAGTGTGTGTACTTTGGGCTGTCTGCCTAT CAAATCCGCTGTGGCTACCCAACCCGTGTGCTCGGGAACTTCCTCACAAAGAGCTACAATTACGTCAACC TCTTCTTGTTTCAAGGGTTCCGCCTGGTCCCATTTCTGACTGAGCTGCGGGCTGTGATGGATTGGGTGTG GACAGATACAACCCTGAGCCTCTCCAGCTGGATTTGTGTGGAGGACATTTACGCGCACATTTTTATCTTA AAGTGCTGGCGAGAGTCGGAAAAGAGATACCCTCAACCCAGGGGTCAGAAGAAGAAGAAAGCCGTGAAGT ACGGCATGGGCGGCATGATCATCGTCCTTCTCATCTGTATCGTCTGGTTTCCTCTGCTCTTCATGTCTTT GATCAAATCTGTCGCAGGGGTTATCAACCAACCCCTGGATGTGTCCGTCACAATTACCCTGGGAGGTTAT CAGCCTATCTTCACAATGAGCGCCCAGCAAAGCCAGCTGAAAGTTATGGATAATTCAAAGTATAATGAAT TTCTAAAATCTTTCGGCCCCAACAGTGGCGCAATGCAATTTCTGGAGAACTATGAAAGGGAAGACGTAAC AGTAGCAGAACTGGAAGGGAACTCGAATTCTTTGTGGACCATCAGCCCTCCCAGTAAGCAGAAAATGATA CAGGAGCTCACCGACCCCAATAGTTGCTTCTCTGTTGTTTTTTCATGGAGTATCCAGAGAAACATGACTT TGGGTGCAAAAGCCGAAATAGCAACGGATAAGCTTTCTTTCCCTCTTGCGGTCGCAACACGGAATAGCAT AGCTAAAATGATTGCTGGCAATGACACAGAAAGTTCAAACACGCCAGTGACAATAGAGAAGATCTACCCA TACTATGTGAAGGCACCCAGCGATTCAAACTCAAAGCCTATAAAACAGCTTCTGTCTGAAAATAATTTCA TGAATATCACCATCATTTTGTTCAGAGACAATGTCACAAAGTCCAATAGTGAGTGGTGGGTTCTCAACCT GACCGGAAGTAGGATATTCAACCAGGGGTCCCAAGCCTTGGAACTGGTGGTCTTCAATGACAAAGTCAGC CCTCCAAGTCTAGGCTTCTTGGCTGGCTATGGTATCATGGGATTGTATGCATCTGTTGTCCTTGTAATTG GGAAATTTGTTCGTGAGTTCTTCAGTGGGATCTCTCATTCCATCATGTTTGAAGAGCTTCCAAATGTGGA CAGAATCTTGAAGTTGTGCACAGATATATTCCTCGTGAGGGAGACAGGGGAGTTGGAACTGGAGGAAGAC CTCTACGCCAAGTTGATATTCCTGTACAGGTCACCAGAAACCATGATCAAGTGGACTAGAGAAAAAACAA ACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001039485
Insert Size	8475 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001039485.4, NP_001034574.4
RefSeq Size	10742 bp
RefSeq ORF	8475 bp
Locus ID	667742
UniProt ID	Q8CD54
Gene Summary	Component of a mechanosensitive channel required for rapidly adapting mechanically activated (MA) currents (PubMed:20813920, PubMed:24717433). Required for Merkel-cell mechanotransduction (PubMed:24717433). Plays a major role in light-touch mechanosensation (PubMed:25471886).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.